<?xml version="1.0" encoding="UTF-8"?><xmi:XMI xmlns:pos="http:///org/u_compare/shared/label/penn/pos.ecore" xmlns:grammatical="http:///org/u_compare/shared/label/penn/function/grammatical.ecore" xmlns:general="http:///org/u_compare/shared/label/penn/pos/general.ecore" xmlns:cas="http:///uima/cas.ecore" xmlns:stanford="http:///org/u_compare/shared/label/stanford.ecore" xmlns:semantic2="http:///uk/ac/nactem/semantic.ecore" xmlns:bioie="http:///org/u_compare/shared/label/penn/pos/bioie.ecore" xmlns:function="http:///org/u_compare/shared/label/penn/function.ecore" xmlns:tcas="http:///uima/tcas.ecore" xmlns:bio="http:///org/u_compare/shared/semantic/bio.ecore" xmlns:syntactic="http:///org/u_compare/shared/syntactic.ecore" xmlns:comparable="http:///org/u_compare/shared/comparable.ecore" xmlns:examples="http:///org/apache/uima/examples.ecore" xmlns:label="http:///org/u_compare/shared/label.ecore" xmlns:xmi="http://www.omg.org/XMI" xmlns:general2="http:///org/u_compare/shared/label/penn/bracket/general.ecore" xmlns:shared="http:///org/u_compare/shared.ecore" xmlns:fragment="http:///org/u_compare/shared/document/fragment.ecore" xmlns:document="http:///org/u_compare/shared/document.ecore" xmlns:bracket="http:///org/u_compare/shared/label/penn/bracket.ecore" xmlns:text="http:///org/u_compare/shared/label/penn/function/text.ecore" xmlns:structure="http:///org/u_compare/shared/document/structure.ecore" xmlns:text2="http:///org/u_compare/shared/document/text.ecore" xmlns:release="http:///org/u_compare/shared/label/penn/pos/release.ecore" xmlns:semantic="http:///org/u_compare/shared/semantic.ecore" xmlns:nontext="http:///org/u_compare/shared/document/nontext.ecore" xmlns:conll="http:///org/u_compare/shared/label/conll.ecore" xmlns:chemical="http:///org/u_compare/shared/semantic/chemical.ecore" xmlns:semantic3="http:///org/u_compare/shared/label/penn/function/semantic.ecore" xmlns:release2="http:///org/u_compare/shared/label/penn/bracket/release.ecore" xmlns:coindex="http:///org/u_compare/shared/label/penn/coindex.ecore" xmi:version="2.0"><cas:NULL xmi:id="0"/><cas:Sofa xmi:id="1" sofaNum="1" sofaID="_InitialView" mimeType="text" sofaString="Three adjacent binding sites for cAMP receptor protein are involved in the activation of the divergent malEp-malKp promoters. &#10;The divergent malEFG and malK-lamB-malM operons in Escherichia coli are controlled by partially overlapping promoters, whose activity depends on the presence of two transcriptional activators, MalT and the cAMP receptor protein (CRP). The 271-base-pair region separating the transcription start points of the promoters malEp and malKp comprises a compact array of binding sites for MalT and CRP. We report the characterization of the in vitro interactions of CRP with its four adjacent binding sites and the analysis of their function in vivo. By using the DNase I footprinting technique, we showed that CRP binds with high affinity to the three malEp-proximal sites and with a low affinity to the fourth site. CRP binding to these sites is not cooperative, even though they are adjacent and located on the same face of the DNA double helix. Each of these sites was destroyed by localized mutagenesis and the residual activity of the promoters was measured in vivo. Mutations in any of the three high-affinity binding sites reduced both malEp and malKp activity. The participation of several adjacent bound CRP molecules in the activation of a promoter is an unprecedented observation and might involve molecular mechanisms quite different from those used in the other CRP-controlled promoters. "/><cas:FSArray xmi:id="463" elements="467 476"/><cas:FSArray xmi:id="470" elements="26"/><cas:FSArray xmi:id="479" elements="353"/><cas:FSArray xmi:id="485" elements="488"/><cas:FSArray xmi:id="491" elements="37"/><cas:FSArray xmi:id="497" elements="502 511 520"/><cas:FSArray xmi:id="505" elements="77"/><cas:FSArray xmi:id="514" elements="48"/><cas:FSArray xmi:id="523" elements="59"/><cas:FSArray xmi:id="529" elements="533 542"/><cas:FSArray xmi:id="536" elements="88"/><cas:FSArray xmi:id="545" elements="95 106"/><cas:FSArray xmi:id="552" elements="557 566 575"/><cas:FSArray xmi:id="560" elements="135"/><cas:FSArray xmi:id="569" elements="124"/><cas:FSArray xmi:id="578" elements="117"/><cas:FSArray xmi:id="584" elements="589 598 607"/><cas:FSArray xmi:id="592" elements="146"/><cas:FSArray xmi:id="601" elements="157"/><cas:FSArray xmi:id="610" elements="164"/><cas:FSArray xmi:id="616" elements="621 630 639"/><cas:FSArray xmi:id="624" elements="146"/><cas:FSArray xmi:id="633" elements="175"/><cas:FSArray xmi:id="642" elements="182"/><cas:FSArray xmi:id="648" elements="653 662 671"/><cas:FSArray xmi:id="656" elements="193"/><cas:FSArray xmi:id="665" elements="215"/><cas:FSArray xmi:id="674" elements="204"/><cas:FSArray xmi:id="680" elements="683"/><cas:FSArray xmi:id="686" elements="222"/><cas:FSArray xmi:id="692" elements="696 705"/><cas:FSArray xmi:id="699" elements="430"/><cas:FSArray xmi:id="708" elements="233"/><cas:FSArray xmi:id="714" elements="717"/><cas:FSArray xmi:id="720" elements="243"/><cas:FSArray xmi:id="729" elements="0 26 37 48 59 66 77 88 95 106 117 124 135 146 157 164 175 182 193 204 215 222 233 243 0 0 0 0 0 0 0 0 0 0 0"/><tcas:DocumentAnnotation xmi:id="8" sofa="1" begin="0" end="1422" language="x-unspecified"/><tcas:DocumentAnnotation xmi:id="21" sofa="1" begin="0" end="1422"/><examples:SourceDocumentInformation xmi:id="13" sofa="1" begin="0" end="1422" uri="/1824723.txt" offsetInSource="0" documentSize="1422" lastSegment="true"/><semantic:LinkedAnnotationSet xmi:id="467" linkingRelation="473" linkedAnnotations="470"/><semantic:LinkedAnnotationSet xmi:id="476" linkingRelation="482" linkedAnnotations="479"/><semantic:LinkedAnnotationSet xmi:id="488" linkingRelation="494" linkedAnnotations="491"/><semantic:LinkedAnnotationSet xmi:id="502" linkingRelation="508" linkedAnnotations="505"/><semantic:LinkedAnnotationSet xmi:id="511" linkingRelation="517" linkedAnnotations="514"/><semantic:LinkedAnnotationSet xmi:id="520" linkingRelation="526" linkedAnnotations="523"/><semantic:LinkedAnnotationSet xmi:id="533" linkingRelation="539" linkedAnnotations="536"/><semantic:LinkedAnnotationSet xmi:id="542" linkingRelation="549" linkedAnnotations="545"/><semantic:LinkedAnnotationSet xmi:id="557" linkingRelation="563" linkedAnnotations="560"/><semantic:LinkedAnnotationSet xmi:id="566" linkingRelation="572" linkedAnnotations="569"/><semantic:LinkedAnnotationSet xmi:id="575" linkingRelation="581" linkedAnnotations="578"/><semantic:LinkedAnnotationSet xmi:id="589" linkingRelation="595" linkedAnnotations="592"/><semantic:LinkedAnnotationSet xmi:id="598" linkingRelation="604" linkedAnnotations="601"/><semantic:LinkedAnnotationSet xmi:id="607" linkingRelation="613" linkedAnnotations="610"/><semantic:LinkedAnnotationSet xmi:id="621" linkingRelation="627" linkedAnnotations="624"/><semantic:LinkedAnnotationSet xmi:id="630" linkingRelation="636" linkedAnnotations="633"/><semantic:LinkedAnnotationSet xmi:id="639" linkingRelation="645" linkedAnnotations="642"/><semantic:LinkedAnnotationSet xmi:id="653" linkingRelation="659" linkedAnnotations="656"/><semantic:LinkedAnnotationSet xmi:id="662" linkingRelation="668" linkedAnnotations="665"/><semantic:LinkedAnnotationSet xmi:id="671" linkingRelation="677" linkedAnnotations="674"/><semantic:LinkedAnnotationSet xmi:id="683" linkingRelation="689" linkedAnnotations="686"/><semantic:LinkedAnnotationSet xmi:id="696" linkingRelation="702" linkedAnnotations="699"/><semantic:LinkedAnnotationSet xmi:id="705" linkingRelation="711" linkedAnnotations="708"/><semantic:LinkedAnnotationSet xmi:id="717" linkingRelation="723" linkedAnnotations="720"/><shared:ExternalReference xmi:id="34" namespace="GREC Concepts" ID="Transcription_Binding_Site"/><shared:ExternalReference xmi:id="45" namespace="GREC Concepts" ID="Promoter"/><shared:ExternalReference xmi:id="56" namespace="GREC Concepts" ID="Operon"/><shared:ExternalReference xmi:id="74" namespace="GREC Concepts" ID="Wild_Type_Bacteria"/><shared:ExternalReference xmi:id="85" namespace="GREC Concepts" ID="Promoter"/><shared:ExternalReference xmi:id="103" namespace="GREC Concepts" ID="Activator"/><shared:ExternalReference xmi:id="114" namespace="GREC Concepts" ID="Activator"/><shared:ExternalReference xmi:id="132" namespace="GREC Concepts" ID="Regulator"/><shared:ExternalReference xmi:id="143" namespace="GREC Concepts" ID="Transcription_Binding_Site"/><shared:ExternalReference xmi:id="154" namespace="GREC Concepts" ID="Regulator"/><shared:ExternalReference xmi:id="172" namespace="GREC Concepts" ID="Promoter"/><shared:ExternalReference xmi:id="190" namespace="GREC Concepts" ID="Promoter"/><shared:ExternalReference xmi:id="201" namespace="GREC Concepts" ID="Regulator"/><shared:ExternalReference xmi:id="212" namespace="GREC Concepts" ID="Promoter"/><shared:ExternalReference xmi:id="230" namespace="GREC Concepts" ID="Transcription_Binding_Site"/><shared:ExternalReference xmi:id="240" namespace="GREC Concepts" ID="Gene_Expression"/><shared:ExternalReference xmi:id="251" namespace="GREC Concepts" ID="Promoter"/><shared:ExternalReference xmi:id="350" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="361" namespace="GREC" ID="Gene_Activation"/><shared:ExternalReference xmi:id="372" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="383" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="394" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="405" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="416" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="427" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="438" namespace="GREC" ID="Mutation"/><shared:ExternalReference xmi:id="449" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="460" namespace="GREC" ID="Gene_Activation"/><shared:ExternalReference xmi:id="473" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="482" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="494" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="508" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="517" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="526" namespace="GREC Concepts" ID="Location"/><shared:ExternalReference xmi:id="539" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="549" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="563" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="572" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="581" namespace="GREC Concepts" ID="Manner"/><shared:ExternalReference xmi:id="595" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="604" namespace="GREC Concepts" ID="Manner"/><shared:ExternalReference xmi:id="613" namespace="GREC Concepts" ID="Destination"/><shared:ExternalReference xmi:id="627" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="636" namespace="GREC Concepts" ID="Manner"/><shared:ExternalReference xmi:id="645" namespace="GREC Concepts" ID="Destination"/><shared:ExternalReference xmi:id="659" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="668" namespace="GREC Concepts" ID="Manner"/><shared:ExternalReference xmi:id="677" namespace="GREC Concepts" ID="Destination"/><shared:ExternalReference xmi:id="689" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="702" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="711" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="723" namespace="GREC Concepts" ID="Theme"/><semantic:EventAnnotation xmi:id="342" sofa="1" begin="59" end="67" externalReference="350" linkedAnnotationSets="463"/><semantic:EventAnnotation xmi:id="353" sofa="1" begin="75" end="85" externalReference="361" linkedAnnotationSets="485"/><semantic:EventAnnotation xmi:id="364" sofa="1" begin="199" end="209" externalReference="372" linkedAnnotationSets="497"/><semantic:EventAnnotation xmi:id="375" sofa="1" begin="261" end="268" externalReference="383" linkedAnnotationSets="529"/><semantic:EventAnnotation xmi:id="386" sofa="1" begin="570" end="582" externalReference="394" linkedAnnotationSets="552"/><semantic:EventAnnotation xmi:id="397" sofa="1" begin="735" end="740" externalReference="405" linkedAnnotationSets="584"/><semantic:EventAnnotation xmi:id="408" sofa="1" begin="735" end="740" externalReference="416" linkedAnnotationSets="616"/><semantic:EventAnnotation xmi:id="419" sofa="1" begin="842" end="849" externalReference="427" linkedAnnotationSets="648"/><semantic:EventAnnotation xmi:id="430" sofa="1" begin="1093" end="1102" externalReference="438" linkedAnnotationSets="680"/><semantic:EventAnnotation xmi:id="441" sofa="1" begin="1151" end="1158" externalReference="449" linkedAnnotationSets="692"/><semantic:EventAnnotation xmi:id="452" sofa="1" begin="1255" end="1265" externalReference="460" linkedAnnotationSets="714"/><comparable:IDArray xmi:id="726" array="729" idType="t"/><semantic2:NucleicAcids xmi:id="26" sofa="1" begin="0" end="28" externalReference="34"/><semantic2:NucleicAcids xmi:id="37" sofa="1" begin="89" end="124" externalReference="45"/><semantic2:NucleicAcids xmi:id="48" sofa="1" begin="127" end="174" externalReference="56"/><semantic2:NucleicAcids xmi:id="77" sofa="1" begin="213" end="244" externalReference="85"/><semantic2:NucleicAcids xmi:id="135" sofa="1" begin="595" end="626" externalReference="143"/><semantic2:NucleicAcids xmi:id="164" sofa="1" begin="763" end="793" externalReference="172"/><semantic2:NucleicAcids xmi:id="182" sofa="1" begin="821" end="836" externalReference="190"/><semantic2:NucleicAcids xmi:id="204" sofa="1" begin="853" end="864" externalReference="212"/><semantic2:NucleicAcids xmi:id="222" sofa="1" begin="1113" end="1150" externalReference="230"/><semantic2:NucleicAcids xmi:id="243" sofa="1" begin="1269" end="1279" externalReference="251"/><semantic2:Proteins xmi:id="95" sofa="1" begin="320" end="324" externalReference="103"/><semantic2:Proteins xmi:id="106" sofa="1" begin="356" end="359" externalReference="114"/><semantic2:Proteins xmi:id="124" sofa="1" begin="586" end="589" externalReference="132"/><semantic2:Proteins xmi:id="146" sofa="1" begin="731" end="734" externalReference="154"/><semantic2:Proteins xmi:id="193" sofa="1" begin="838" end="841" externalReference="201"/><semantic2:LivingSystems xmi:id="66" sofa="1" begin="178" end="194" externalReference="74"/><semantic2:Processes xmi:id="233" sofa="1" begin="1164" end="1188" externalReference="240"/><semantic2:EventArgumentSpan xmi:id="59" sofa="1" begin="175" end="194"/><semantic2:EventArgumentSpan xmi:id="88" sofa="1" begin="246" end="260"/><semantic2:EventArgumentSpan xmi:id="117" sofa="1" begin="561" end="569"/><semantic2:EventArgumentSpan xmi:id="157" sofa="1" begin="746" end="759"/><semantic2:EventArgumentSpan xmi:id="175" sofa="1" begin="803" end="817"/><semantic2:EventArgumentSpan xmi:id="215" sofa="1" begin="868" end="883"/><cas:View sofa="1" members="463 470 479 485 491 497 505 514 523 529 536 545 552 560 569 578 584 592 601 610 616 624 633 642 648 656 665 674 680 686 692 699 708 714 720 729 8 21 13 467 476 488 502 511 520 533 542 557 566 575 589 598 607 621 630 639 653 662 671 683 696 705 717 34 45 56 74 85 103 114 132 143 154 172 190 201 212 230 240 251 350 361 372 383 394 405 416 427 438 449 460 473 482 494 508 517 526 539 549 563 572 581 595 604 613 627 636 645 659 668 677 689 702 711 723 342 353 364 375 386 397 408 419 430 441 452 726 26 37 48 77 135 164 182 204 222 243 95 106 124 146 193 66 233 59 88 117 157 175 215"/></xmi:XMI>