<?xml version="1.0" encoding="UTF-8"?><xmi:XMI xmlns:pos="http:///org/u_compare/shared/label/penn/pos.ecore" xmlns:grammatical="http:///org/u_compare/shared/label/penn/function/grammatical.ecore" xmlns:general="http:///org/u_compare/shared/label/penn/pos/general.ecore" xmlns:cas="http:///uima/cas.ecore" xmlns:stanford="http:///org/u_compare/shared/label/stanford.ecore" xmlns:semantic2="http:///uk/ac/nactem/semantic.ecore" xmlns:bioie="http:///org/u_compare/shared/label/penn/pos/bioie.ecore" xmlns:function="http:///org/u_compare/shared/label/penn/function.ecore" xmlns:tcas="http:///uima/tcas.ecore" xmlns:bio="http:///org/u_compare/shared/semantic/bio.ecore" xmlns:syntactic="http:///org/u_compare/shared/syntactic.ecore" xmlns:comparable="http:///org/u_compare/shared/comparable.ecore" xmlns:examples="http:///org/apache/uima/examples.ecore" xmlns:label="http:///org/u_compare/shared/label.ecore" xmlns:xmi="http://www.omg.org/XMI" xmlns:general2="http:///org/u_compare/shared/label/penn/bracket/general.ecore" xmlns:shared="http:///org/u_compare/shared.ecore" xmlns:fragment="http:///org/u_compare/shared/document/fragment.ecore" xmlns:document="http:///org/u_compare/shared/document.ecore" xmlns:bracket="http:///org/u_compare/shared/label/penn/bracket.ecore" xmlns:text="http:///org/u_compare/shared/label/penn/function/text.ecore" xmlns:structure="http:///org/u_compare/shared/document/structure.ecore" xmlns:text2="http:///org/u_compare/shared/document/text.ecore" xmlns:release="http:///org/u_compare/shared/label/penn/pos/release.ecore" xmlns:semantic="http:///org/u_compare/shared/semantic.ecore" xmlns:nontext="http:///org/u_compare/shared/document/nontext.ecore" xmlns:conll="http:///org/u_compare/shared/label/conll.ecore" xmlns:chemical="http:///org/u_compare/shared/semantic/chemical.ecore" xmlns:semantic3="http:///org/u_compare/shared/label/penn/function/semantic.ecore" xmlns:release2="http:///org/u_compare/shared/label/penn/bracket/release.ecore" xmlns:coindex="http:///org/u_compare/shared/label/penn/coindex.ecore" xmi:version="2.0"><cas:NULL xmi:id="0"/><cas:Sofa xmi:id="1" sofaNum="1" sofaID="_InitialView" mimeType="text" sofaString="Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1. &#10;Recent transcription mapping efforts within chromosome 17q21 have led to the identification of a human homolog of the Drosophila gene Enhancer of zeste, E(z). A member of the Polycomb group (Pc-G) of proteins, Drosophila E(z) acts as a negative regulator of the segment identity genes of the Antennapedia and Bithorax complexes. Here we report the full-length protein coding sequence of human EZH1 (Enhancer of zeste homolog 1) and compare the respective protein sequences in both species. EZH1 encodes a protein of 747 amino acids that displays 55% amino acid identity overall (70% similarity) with Drosophila E(z). The strongest homology was noted (79% identity, 89% similarity) within the carboxy-terminal 245 amino acids, including the SET domain, a region of E(z) also conserved in other Drosophila proteins with roles in development and/or chromatin structure. A large Cysrich region with a novel spatial pattern of cysteine residues was also conserved in both EZH1 and E(z). The strong sequence conservation suggest potential roles for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that stably maintain heterochromatin. EZH1 is expressed as two major transcripts in all adult and fetal human tissues surveyed; comparison of cloned cDNAs suggests that alternative splicing may account for at least part of the transcript size difference. Analysis of one cDNA revealed an unusual splicing event involving EZH1 and a tandemly linked gene GPR2 and suggests a potential mechanism for modifying the EZH1 protein in the conserved C-terminal domain. The sequence and isolated cDNAs will provide useful reagents for determining the function of EZH1 and the importance of the evolutionarily conserved domains. "/><cas:FSArray xmi:id="282" elements="286 295"/><cas:FSArray xmi:id="289" elements="26"/><cas:FSArray xmi:id="298" elements="37"/><cas:FSArray xmi:id="304" elements="308 317"/><cas:FSArray xmi:id="311" elements="48"/><cas:FSArray xmi:id="320" elements="59"/><cas:FSArray xmi:id="326" elements="330 339"/><cas:FSArray xmi:id="333" elements="70"/><cas:FSArray xmi:id="342" elements="81"/><cas:FSArray xmi:id="348" elements="353 362 371"/><cas:FSArray xmi:id="356" elements="88"/><cas:FSArray xmi:id="365" elements="110"/><cas:FSArray xmi:id="374" elements="99"/><cas:FSArray xmi:id="380" elements="384 393"/><cas:FSArray xmi:id="387" elements="128"/><cas:FSArray xmi:id="396" elements="135 146"/><cas:FSArray xmi:id="403" elements="406"/><cas:FSArray xmi:id="409" elements="157"/><cas:FSArray xmi:id="418" elements="0 26 37 48 59 70 81 88 99 110 117 128 135 146 157 0 0 0 0 0 0"/><tcas:DocumentAnnotation xmi:id="8" sofa="1" begin="0" end="1845" language="x-unspecified"/><tcas:DocumentAnnotation xmi:id="21" sofa="1" begin="0" end="1845"/><examples:SourceDocumentInformation xmi:id="13" sofa="1" begin="0" end="1845" uri="/8921387.txt" offsetInSource="0" documentSize="1845" lastSegment="true"/><semantic:LinkedAnnotationSet xmi:id="286" linkingRelation="292" linkedAnnotations="289"/><semantic:LinkedAnnotationSet xmi:id="295" linkingRelation="301" linkedAnnotations="298"/><semantic:LinkedAnnotationSet xmi:id="308" linkingRelation="314" linkedAnnotations="311"/><semantic:LinkedAnnotationSet xmi:id="317" linkingRelation="323" linkedAnnotations="320"/><semantic:LinkedAnnotationSet xmi:id="330" linkingRelation="336" linkedAnnotations="333"/><semantic:LinkedAnnotationSet xmi:id="339" linkingRelation="345" linkedAnnotations="342"/><semantic:LinkedAnnotationSet xmi:id="353" linkingRelation="359" linkedAnnotations="356"/><semantic:LinkedAnnotationSet xmi:id="362" linkingRelation="368" linkedAnnotations="365"/><semantic:LinkedAnnotationSet xmi:id="371" linkingRelation="377" linkedAnnotations="374"/><semantic:LinkedAnnotationSet xmi:id="384" linkingRelation="390" linkedAnnotations="387"/><semantic:LinkedAnnotationSet xmi:id="393" linkingRelation="400" linkedAnnotations="396"/><semantic:LinkedAnnotationSet xmi:id="406" linkingRelation="412" linkedAnnotations="409"/><shared:ExternalReference xmi:id="34" namespace="GREC Concepts" ID="Regulator"/><shared:ExternalReference xmi:id="45" namespace="GREC Concepts" ID="Regulator"/><shared:ExternalReference xmi:id="56" namespace="GREC Concepts" ID="Gene"/><shared:ExternalReference xmi:id="67" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="78" namespace="GREC Concepts" ID="Protein_Complex"/><shared:ExternalReference xmi:id="96" namespace="GREC Concepts" ID="Gene"/><shared:ExternalReference xmi:id="107" namespace="GREC Concepts" ID="mRNA"/><shared:ExternalReference xmi:id="125" namespace="GREC Concepts" ID="Tissue"/><shared:ExternalReference xmi:id="143" namespace="GREC Concepts" ID="Gene"/><shared:ExternalReference xmi:id="154" namespace="GREC Concepts" ID="Gene"/><shared:ExternalReference xmi:id="165" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="224" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="235" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="246" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="257" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="268" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="279" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="292" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="301" namespace="GREC Concepts" ID="Descriptive-Agent"/><shared:ExternalReference xmi:id="314" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="323" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="336" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="345" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="359" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="368" namespace="GREC Concepts" ID="Location"/><shared:ExternalReference xmi:id="377" namespace="GREC Concepts" ID="Descriptive-Theme"/><shared:ExternalReference xmi:id="390" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="400" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="412" namespace="GREC Concepts" ID="Theme"/><semantic:EventAnnotation xmi:id="216" sofa="1" begin="313" end="317" externalReference="224" linkedAnnotationSets="282"/><semantic:EventAnnotation xmi:id="227" sofa="1" begin="582" end="589" externalReference="235" linkedAnnotationSets="304"/><semantic:EventAnnotation xmi:id="238" sofa="1" begin="1239" end="1247" externalReference="246" linkedAnnotationSets="326"/><semantic:EventAnnotation xmi:id="249" sofa="1" begin="1273" end="1282" externalReference="257" linkedAnnotationSets="348"/><semantic:EventAnnotation xmi:id="260" sofa="1" begin="1538" end="1547" externalReference="268" linkedAnnotationSets="380"/><semantic:EventAnnotation xmi:id="271" sofa="1" begin="1624" end="1633" externalReference="279" linkedAnnotationSets="403"/><comparable:IDArray xmi:id="415" array="418" idType="t"/><semantic2:NucleicAcids xmi:id="48" sofa="1" begin="577" end="581" externalReference="56"/><semantic2:NucleicAcids xmi:id="88" sofa="1" begin="1265" end="1269" externalReference="96"/><semantic2:NucleicAcids xmi:id="99" sofa="1" begin="1286" end="1307" externalReference="107"/><semantic2:NucleicAcids xmi:id="135" sofa="1" begin="1548" end="1552" externalReference="143"/><semantic2:NucleicAcids xmi:id="146" sofa="1" begin="1557" end="1584" externalReference="154"/><semantic2:Proteins xmi:id="26" sofa="1" begin="297" end="312" externalReference="34"/><semantic2:Proteins xmi:id="37" sofa="1" begin="321" end="341" externalReference="45"/><semantic2:Proteins xmi:id="59" sofa="1" begin="590" end="599" externalReference="67"/><semantic2:Proteins xmi:id="70" sofa="1" begin="1209" end="1226" externalReference="78"/><semantic2:Proteins xmi:id="157" sofa="1" begin="1634" end="1650" externalReference="165"/><semantic2:LivingSystems xmi:id="117" sofa="1" begin="1311" end="1344" externalReference="125"/><semantic2:EventArgumentSpan xmi:id="81" sofa="1" begin="1248" end="1263"/><semantic2:EventArgumentSpan xmi:id="110" sofa="1" begin="1308" end="1344"/><semantic2:EventArgumentSpan xmi:id="128" sofa="1" begin="1512" end="1537"/><cas:View sofa="1" members="282 289 298 304 311 320 326 333 342 348 356 365 374 380 387 396 403 409 418 8 21 13 286 295 308 317 330 339 353 362 371 384 393 406 34 45 56 67 78 96 107 125 143 154 165 224 235 246 257 268 279 292 301 314 323 336 345 359 368 377 390 400 412 216 227 238 249 260 271 415 48 88 99 135 146 26 37 59 70 157 117 81 110 128"/></xmi:XMI>