<?xml version="1.0" encoding="UTF-8"?><xmi:XMI xmlns:pos="http:///org/u_compare/shared/label/penn/pos.ecore" xmlns:grammatical="http:///org/u_compare/shared/label/penn/function/grammatical.ecore" xmlns:general="http:///org/u_compare/shared/label/penn/pos/general.ecore" xmlns:cas="http:///uima/cas.ecore" xmlns:stanford="http:///org/u_compare/shared/label/stanford.ecore" xmlns:semantic2="http:///uk/ac/nactem/semantic.ecore" xmlns:bioie="http:///org/u_compare/shared/label/penn/pos/bioie.ecore" xmlns:function="http:///org/u_compare/shared/label/penn/function.ecore" xmlns:tcas="http:///uima/tcas.ecore" xmlns:bio="http:///org/u_compare/shared/semantic/bio.ecore" xmlns:syntactic="http:///org/u_compare/shared/syntactic.ecore" xmlns:comparable="http:///org/u_compare/shared/comparable.ecore" xmlns:examples="http:///org/apache/uima/examples.ecore" xmlns:label="http:///org/u_compare/shared/label.ecore" xmlns:xmi="http://www.omg.org/XMI" xmlns:general2="http:///org/u_compare/shared/label/penn/bracket/general.ecore" xmlns:shared="http:///org/u_compare/shared.ecore" xmlns:fragment="http:///org/u_compare/shared/document/fragment.ecore" xmlns:document="http:///org/u_compare/shared/document.ecore" xmlns:bracket="http:///org/u_compare/shared/label/penn/bracket.ecore" xmlns:text="http:///org/u_compare/shared/label/penn/function/text.ecore" xmlns:structure="http:///org/u_compare/shared/document/structure.ecore" xmlns:text2="http:///org/u_compare/shared/document/text.ecore" xmlns:release="http:///org/u_compare/shared/label/penn/pos/release.ecore" xmlns:semantic="http:///org/u_compare/shared/semantic.ecore" xmlns:nontext="http:///org/u_compare/shared/document/nontext.ecore" xmlns:conll="http:///org/u_compare/shared/label/conll.ecore" xmlns:chemical="http:///org/u_compare/shared/semantic/chemical.ecore" xmlns:semantic3="http:///org/u_compare/shared/label/penn/function/semantic.ecore" xmlns:release2="http:///org/u_compare/shared/label/penn/bracket/release.ecore" xmlns:coindex="http:///org/u_compare/shared/label/penn/coindex.ecore" xmi:version="2.0"><cas:NULL xmi:id="0"/><cas:Sofa xmi:id="1" sofaNum="1" sofaID="_InitialView" mimeType="text" sofaString="Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. &#10;The glucocorticoid receptor (GR), a member of the nuclear receptor superfamily, mediates the effects of glucocorticoids. It is known that 14-3-3 family proteins interact with GR and regulate its transcriptional activity. They also bind to several molecules and influence many cellular events by altering their subcellular localization and/or acting as a chaperone. Recently, it has been proposed that ligand-activated degradation of GR occurs via the ubiquitin-proteasomal degradation pathway and that inhibition of proteasomal activity induces up-regulation of GR and enhances the transcriptional activity of GR. To examine the function of 14-3-3eta in the glucocorticoid-dependent signal pathway, we studied the regulatory role of 14-3-3eta in ligand-induced GR transcriptional activation. 14-3-3eta Enhanced the transcriptional activity of GR, and the levels of GR were higher in cells transfected with the 14-3-3eta expression vector in response to glucocorticoid. The GR level increased in both cytosol and nucleus, and endogenous GR was also elevated by 14-3-3eta in HeLa cells. 14-3-3eta Inhibited ligand-induced down-regulation of GR. Proteasomal inhibition did not induce any synergistic effect on the 14-3-3eta-induced increase in GR in response to glucocorticoid, and inhibition of translation did not block elevation of GR by 14-3-3eta, indicating that 14-3-3eta induces stabilization of GR. These results suggest that 14-3-3eta functions as a positive regulator in the glucocorticoid signal pathway by blocking the degradation of GR and inducing an elevation of GR, thus enhancing the transcriptional activity of GR. "/><cas:FSArray xmi:id="512" elements="515"/><cas:FSArray xmi:id="518" elements="26"/><cas:FSArray xmi:id="524" elements="528 537"/><cas:FSArray xmi:id="531" elements="33"/><cas:FSArray xmi:id="540" elements="44"/><cas:FSArray xmi:id="546" elements="550 559"/><cas:FSArray xmi:id="553" elements="51"/><cas:FSArray xmi:id="562" elements="380"/><cas:FSArray xmi:id="568" elements="572 581"/><cas:FSArray xmi:id="575" elements="51"/><cas:FSArray xmi:id="584" elements="58"/><cas:FSArray xmi:id="590" elements="594 603"/><cas:FSArray xmi:id="597" elements="51"/><cas:FSArray xmi:id="606" elements="69"/><cas:FSArray xmi:id="612" elements="615"/><cas:FSArray xmi:id="618" elements="87"/><cas:FSArray xmi:id="624" elements="628 637"/><cas:FSArray xmi:id="631" elements="94"/><cas:FSArray xmi:id="640" elements="105"/><cas:FSArray xmi:id="646" elements="651 660 669"/><cas:FSArray xmi:id="654" elements="130"/><cas:FSArray xmi:id="663" elements="123"/><cas:FSArray xmi:id="672" elements="137"/><cas:FSArray xmi:id="678" elements="682 691"/><cas:FSArray xmi:id="685" elements="144"/><cas:FSArray xmi:id="694" elements="446"/><cas:FSArray xmi:id="700" elements="704 713"/><cas:FSArray xmi:id="707" elements="162"/><cas:FSArray xmi:id="716" elements="155"/><cas:FSArray xmi:id="722" elements="725"/><cas:FSArray xmi:id="728" elements="173"/><cas:FSArray xmi:id="734" elements="738 747"/><cas:FSArray xmi:id="741" elements="191"/><cas:FSArray xmi:id="750" elements="180"/><cas:FSArray xmi:id="756" elements="760 769"/><cas:FSArray xmi:id="763" elements="202"/><cas:FSArray xmi:id="772" elements="490"/><cas:FSArray xmi:id="778" elements="781"/><cas:FSArray xmi:id="784" elements="209"/><cas:FSArray xmi:id="790" elements="794 803"/><cas:FSArray xmi:id="797" elements="202"/><cas:FSArray xmi:id="806" elements="220"/><cas:FSArray xmi:id="815" elements="0 26 33 44 51 58 69 76 87 94 105 112 123 130 137 144 155 162 173 180 191 202 209 220 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0"/><tcas:DocumentAnnotation xmi:id="8" sofa="1" begin="0" end="1733" language="x-unspecified"/><tcas:DocumentAnnotation xmi:id="21" sofa="1" begin="0" end="1733"/><examples:SourceDocumentInformation xmi:id="13" sofa="1" begin="0" end="1733" uri="/15790729.txt" offsetInSource="0" documentSize="1733" lastSegment="true"/><semantic:LinkedAnnotationSet xmi:id="515" linkingRelation="521" linkedAnnotations="518"/><semantic:LinkedAnnotationSet xmi:id="528" linkingRelation="534" linkedAnnotations="531"/><semantic:LinkedAnnotationSet xmi:id="537" linkingRelation="543" linkedAnnotations="540"/><semantic:LinkedAnnotationSet xmi:id="550" linkingRelation="556" linkedAnnotations="553"/><semantic:LinkedAnnotationSet xmi:id="559" linkingRelation="565" linkedAnnotations="562"/><semantic:LinkedAnnotationSet xmi:id="572" linkingRelation="578" linkedAnnotations="575"/><semantic:LinkedAnnotationSet xmi:id="581" linkingRelation="587" linkedAnnotations="584"/><semantic:LinkedAnnotationSet xmi:id="594" linkingRelation="600" linkedAnnotations="597"/><semantic:LinkedAnnotationSet xmi:id="603" linkingRelation="609" linkedAnnotations="606"/><semantic:LinkedAnnotationSet xmi:id="615" linkingRelation="621" linkedAnnotations="618"/><semantic:LinkedAnnotationSet xmi:id="628" linkingRelation="634" linkedAnnotations="631"/><semantic:LinkedAnnotationSet xmi:id="637" linkingRelation="643" linkedAnnotations="640"/><semantic:LinkedAnnotationSet xmi:id="651" linkingRelation="657" linkedAnnotations="654"/><semantic:LinkedAnnotationSet xmi:id="660" linkingRelation="666" linkedAnnotations="663"/><semantic:LinkedAnnotationSet xmi:id="669" linkingRelation="675" linkedAnnotations="672"/><semantic:LinkedAnnotationSet xmi:id="682" linkingRelation="688" linkedAnnotations="685"/><semantic:LinkedAnnotationSet xmi:id="691" linkingRelation="697" linkedAnnotations="694"/><semantic:LinkedAnnotationSet xmi:id="704" linkingRelation="710" linkedAnnotations="707"/><semantic:LinkedAnnotationSet xmi:id="713" linkingRelation="719" linkedAnnotations="716"/><semantic:LinkedAnnotationSet xmi:id="725" linkingRelation="731" linkedAnnotations="728"/><semantic:LinkedAnnotationSet xmi:id="738" linkingRelation="744" linkedAnnotations="741"/><semantic:LinkedAnnotationSet xmi:id="747" linkingRelation="753" linkedAnnotations="750"/><semantic:LinkedAnnotationSet xmi:id="760" linkingRelation="766" linkedAnnotations="763"/><semantic:LinkedAnnotationSet xmi:id="769" linkingRelation="775" linkedAnnotations="772"/><semantic:LinkedAnnotationSet xmi:id="781" linkingRelation="787" linkedAnnotations="784"/><semantic:LinkedAnnotationSet xmi:id="794" linkingRelation="800" linkedAnnotations="797"/><semantic:LinkedAnnotationSet xmi:id="803" linkingRelation="809" linkedAnnotations="806"/><shared:ExternalReference xmi:id="41" namespace="GREC Concepts" ID="Regulator"/><shared:ExternalReference xmi:id="66" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="84" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="102" namespace="GREC Concepts" ID="Regulator"/><shared:ExternalReference xmi:id="120" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="152" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="170" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="188" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="199" namespace="GREC Concepts" ID="Regulator"/><shared:ExternalReference xmi:id="217" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="355" namespace="GREC" ID="Gene_Activation"/><shared:ExternalReference xmi:id="366" namespace="GREC" ID="Regulation"/><shared:ExternalReference xmi:id="377" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="388" namespace="GREC" ID="Regulation"/><shared:ExternalReference xmi:id="399" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="410" namespace="GREC" ID="Gene_Activation"/><shared:ExternalReference xmi:id="421" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="432" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="443" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="454" namespace="GREC" ID="Regulation"/><shared:ExternalReference xmi:id="465" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="476" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="487" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="498" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="509" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="521" namespace="GREC Concepts" ID="Manner"/><shared:ExternalReference xmi:id="534" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="543" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="556" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="565" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="578" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="587" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="600" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="609" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="621" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="634" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="643" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="657" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="666" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="675" namespace="GREC Concepts" ID="Location"/><shared:ExternalReference xmi:id="688" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="697" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="710" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="719" namespace="GREC Concepts" ID="Manner"/><shared:ExternalReference xmi:id="731" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="744" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="753" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="766" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="775" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="787" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="800" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="809" namespace="GREC Concepts" ID="Theme"/><semantic:EventAnnotation xmi:id="347" sofa="1" begin="90" end="100" externalReference="355" linkedAnnotationSets="512"/><semantic:EventAnnotation xmi:id="358" sofa="1" begin="285" end="293" externalReference="366" linkedAnnotationSets="524"/><semantic:EventAnnotation xmi:id="369" sofa="1" begin="640" end="647" externalReference="377" linkedAnnotationSets="546"/><semantic:EventAnnotation xmi:id="380" sofa="1" begin="648" end="661" externalReference="388" linkedAnnotationSets="568"/><semantic:EventAnnotation xmi:id="391" sofa="1" begin="672" end="680" externalReference="399" linkedAnnotationSets="590"/><semantic:EventAnnotation xmi:id="402" sofa="1" begin="883" end="893" externalReference="410" linkedAnnotationSets="612"/><semantic:EventAnnotation xmi:id="413" sofa="1" begin="905" end="913" externalReference="421" linkedAnnotationSets="624"/><semantic:EventAnnotation xmi:id="424" sofa="1" begin="1151" end="1159" externalReference="432" linkedAnnotationSets="646"/><semantic:EventAnnotation xmi:id="435" sofa="1" begin="1198" end="1207" externalReference="443" linkedAnnotationSets="678"/><semantic:EventAnnotation xmi:id="446" sofa="1" begin="1223" end="1238" externalReference="454" linkedAnnotationSets="700"/><semantic:EventAnnotation xmi:id="457" sofa="1" begin="1382" end="1392" externalReference="465" linkedAnnotationSets="722"/><semantic:EventAnnotation xmi:id="468" sofa="1" begin="1422" end="1431" externalReference="476" linkedAnnotationSets="734"/><semantic:EventAnnotation xmi:id="479" sofa="1" begin="1653" end="1661" externalReference="487" linkedAnnotationSets="756"/><semantic:EventAnnotation xmi:id="490" sofa="1" begin="1665" end="1674" externalReference="498" linkedAnnotationSets="778"/><semantic:EventAnnotation xmi:id="501" sofa="1" begin="1687" end="1696" externalReference="509" linkedAnnotationSets="790"/><comparable:IDArray xmi:id="812" array="815" idType="t"/><semantic2:Proteins xmi:id="33" sofa="1" begin="241" end="263" externalReference="41"/><semantic2:Proteins xmi:id="58" sofa="1" begin="665" end="667" externalReference="66"/><semantic2:Proteins xmi:id="76" sofa="1" begin="713" end="715" externalReference="84"/><semantic2:Proteins xmi:id="94" sofa="1" begin="895" end="904" externalReference="102"/><semantic2:Proteins xmi:id="112" sofa="1" begin="946" end="948" externalReference="120"/><semantic2:Proteins xmi:id="144" sofa="1" begin="1188" end="1197" externalReference="152"/><semantic2:Proteins xmi:id="162" sofa="1" begin="1242" end="1244" externalReference="170"/><semantic2:Proteins xmi:id="180" sofa="1" begin="1435" end="1437" externalReference="188"/><semantic2:Proteins xmi:id="191" sofa="1" begin="1441" end="1450" externalReference="199"/><semantic2:Proteins xmi:id="209" sofa="1" begin="1678" end="1680" externalReference="217"/><semantic2:EventArgumentSpan xmi:id="26" sofa="1" begin="46" end="89"/><semantic2:EventArgumentSpan xmi:id="44" sofa="1" begin="294" end="322"/><semantic2:EventArgumentSpan xmi:id="51" sofa="1" begin="605" end="615"/><semantic2:EventArgumentSpan xmi:id="69" sofa="1" begin="681" end="715"/><semantic2:EventArgumentSpan xmi:id="87" sofa="1" begin="849" end="882"/><semantic2:EventArgumentSpan xmi:id="105" sofa="1" begin="914" end="948"/><semantic2:EventArgumentSpan xmi:id="123" sofa="1" begin="1128" end="1141"/><semantic2:EventArgumentSpan xmi:id="130" sofa="1" begin="1163" end="1172"/><semantic2:EventArgumentSpan xmi:id="137" sofa="1" begin="1173" end="1186"/><semantic2:EventArgumentSpan xmi:id="155" sofa="1" begin="1208" end="1222"/><semantic2:EventArgumentSpan xmi:id="173" sofa="1" begin="1396" end="1407"/><semantic2:EventArgumentSpan xmi:id="202" sofa="1" begin="1534" end="1543"/><semantic2:EventArgumentSpan xmi:id="220" sofa="1" begin="1697" end="1731"/><cas:View sofa="1" members="512 518 524 531 540 546 553 562 568 575 584 590 597 606 612 618 624 631 640 646 654 663 672 678 685 694 700 707 716 722 728 734 741 750 756 763 772 778 784 790 797 806 815 8 21 13 515 528 537 550 559 572 581 594 603 615 628 637 651 660 669 682 691 704 713 725 738 747 760 769 781 794 803 41 66 84 102 120 152 170 188 199 217 355 366 377 388 399 410 421 432 443 454 465 476 487 498 509 521 534 543 556 565 578 587 600 609 621 634 643 657 666 675 688 697 710 719 731 744 753 766 775 787 800 809 347 358 369 380 391 402 413 424 435 446 457 468 479 490 501 812 33 58 76 94 112 144 162 180 191 209 26 44 51 69 87 105 123 130 137 155 173 202 220"/></xmi:XMI>