<?xml version="1.0" encoding="UTF-8"?><xmi:XMI xmlns:pos="http:///org/u_compare/shared/label/penn/pos.ecore" xmlns:grammatical="http:///org/u_compare/shared/label/penn/function/grammatical.ecore" xmlns:general="http:///org/u_compare/shared/label/penn/pos/general.ecore" xmlns:cas="http:///uima/cas.ecore" xmlns:stanford="http:///org/u_compare/shared/label/stanford.ecore" xmlns:semantic2="http:///uk/ac/nactem/semantic.ecore" xmlns:bioie="http:///org/u_compare/shared/label/penn/pos/bioie.ecore" xmlns:function="http:///org/u_compare/shared/label/penn/function.ecore" xmlns:tcas="http:///uima/tcas.ecore" xmlns:bio="http:///org/u_compare/shared/semantic/bio.ecore" xmlns:syntactic="http:///org/u_compare/shared/syntactic.ecore" xmlns:comparable="http:///org/u_compare/shared/comparable.ecore" xmlns:examples="http:///org/apache/uima/examples.ecore" xmlns:label="http:///org/u_compare/shared/label.ecore" xmlns:xmi="http://www.omg.org/XMI" xmlns:general2="http:///org/u_compare/shared/label/penn/bracket/general.ecore" xmlns:shared="http:///org/u_compare/shared.ecore" xmlns:fragment="http:///org/u_compare/shared/document/fragment.ecore" xmlns:document="http:///org/u_compare/shared/document.ecore" xmlns:bracket="http:///org/u_compare/shared/label/penn/bracket.ecore" xmlns:text="http:///org/u_compare/shared/label/penn/function/text.ecore" xmlns:structure="http:///org/u_compare/shared/document/structure.ecore" xmlns:text2="http:///org/u_compare/shared/document/text.ecore" xmlns:release="http:///org/u_compare/shared/label/penn/pos/release.ecore" xmlns:semantic="http:///org/u_compare/shared/semantic.ecore" xmlns:nontext="http:///org/u_compare/shared/document/nontext.ecore" xmlns:conll="http:///org/u_compare/shared/label/conll.ecore" xmlns:chemical="http:///org/u_compare/shared/semantic/chemical.ecore" xmlns:semantic3="http:///org/u_compare/shared/label/penn/function/semantic.ecore" xmlns:release2="http:///org/u_compare/shared/label/penn/bracket/release.ecore" xmlns:coindex="http:///org/u_compare/shared/label/penn/coindex.ecore" xmi:version="2.0"><cas:NULL xmi:id="0"/><cas:Sofa xmi:id="1" sofaNum="1" sofaID="_InitialView" mimeType="text" sofaString="RET finger protein is a transcriptional repressor and interacts with enhancer of polycomb that has dual transcriptional functions. &#10;RET finger protein (RFP) belongs to the large B-box RING finger protein family and is known to become oncogenic by fusion with RET tyrosine kinase. Although RFP is reported to be a nuclear protein that is present in the nuclear matrix, its function is largely unknown. Here we show that RFP interacts with Enhancer of Polycomb (EPC) and strongly represses the gene transcription. Yeast two-hybrid assays revealed that the coiled-coil domain of RFP was associated with the EPcA domain and the carboxyl-terminal region of EPC. In addition, both proteins were co-precipitated from the lysates of human cells and mostly colocalized in the nucleus. Using the luciferase reporter-gene assay, we found that they repress the gene transcription activity independent of the differences of enhancers and promoters used, although the repressive activity of RFP was much stronger than that of EPC. The coiled-coil domain of RFP and the carboxyl-terminal region of EPC were most important for the repressive activity of each protein, whereas the EPcA domain had the transcription activating ability that is unique as the Polycomb group protein function. These results suggested that RFP may be involved in the epigenetic gene silencing mechanism cooperating with Polycomb group proteins and that EPC is a unique molecule with both repressive and transactivating activities. "/><cas:FSArray xmi:id="244" elements="248 257"/><cas:FSArray xmi:id="251" elements="26"/><cas:FSArray xmi:id="260" elements="37"/><cas:FSArray xmi:id="266" elements="270 279"/><cas:FSArray xmi:id="273" elements="48"/><cas:FSArray xmi:id="282" elements="59"/><cas:FSArray xmi:id="288" elements="293 302 311"/><cas:FSArray xmi:id="296" elements="48"/><cas:FSArray xmi:id="305" elements="211"/><cas:FSArray xmi:id="314" elements="70"/><cas:FSArray xmi:id="320" elements="323"/><cas:FSArray xmi:id="326" elements="77"/><cas:FSArray xmi:id="332" elements="336 345"/><cas:FSArray xmi:id="339" elements="88"/><cas:FSArray xmi:id="348" elements="95"/><cas:FSArray xmi:id="354" elements="358 367"/><cas:FSArray xmi:id="361" elements="112"/><cas:FSArray xmi:id="370" elements="123"/><cas:FSArray xmi:id="379" elements="0 26 37 48 59 70 77 88 95 102 112 123 0 0 0 0 0 0"/><tcas:DocumentAnnotation xmi:id="8" sofa="1" begin="0" end="1492" language="x-unspecified"/><tcas:DocumentAnnotation xmi:id="21" sofa="1" begin="0" end="1492"/><examples:SourceDocumentInformation xmi:id="13" sofa="1" begin="0" end="1492" uri="/10976108.txt" offsetInSource="0" documentSize="1492" lastSegment="true"/><semantic:LinkedAnnotationSet xmi:id="248" linkingRelation="254" linkedAnnotations="251"/><semantic:LinkedAnnotationSet xmi:id="257" linkingRelation="263" linkedAnnotations="260"/><semantic:LinkedAnnotationSet xmi:id="270" linkingRelation="276" linkedAnnotations="273"/><semantic:LinkedAnnotationSet xmi:id="279" linkingRelation="285" linkedAnnotations="282"/><semantic:LinkedAnnotationSet xmi:id="293" linkingRelation="299" linkedAnnotations="296"/><semantic:LinkedAnnotationSet xmi:id="302" linkingRelation="308" linkedAnnotations="305"/><semantic:LinkedAnnotationSet xmi:id="311" linkingRelation="317" linkedAnnotations="314"/><semantic:LinkedAnnotationSet xmi:id="323" linkingRelation="329" linkedAnnotations="326"/><semantic:LinkedAnnotationSet xmi:id="336" linkingRelation="342" linkedAnnotations="339"/><semantic:LinkedAnnotationSet xmi:id="345" linkingRelation="351" linkedAnnotations="348"/><semantic:LinkedAnnotationSet xmi:id="358" linkingRelation="364" linkedAnnotations="361"/><semantic:LinkedAnnotationSet xmi:id="367" linkingRelation="373" linkedAnnotations="370"/><shared:ExternalReference xmi:id="34" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="45" namespace="GREC Concepts" ID="Enhancer"/><shared:ExternalReference xmi:id="56" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="67" namespace="GREC Concepts" ID="Enhancer"/><shared:ExternalReference xmi:id="85" namespace="GREC Concepts" ID="Gene"/><shared:ExternalReference xmi:id="109" namespace="GREC Concepts" ID="Transcription"/><shared:ExternalReference xmi:id="120" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="186" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="197" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="208" namespace="GREC" ID="Gene_Repression"/><shared:ExternalReference xmi:id="219" namespace="GREC" ID="Transcription"/><shared:ExternalReference xmi:id="230" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="241" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="254" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="263" namespace="GREC Concepts" ID="Descriptive-Agent"/><shared:ExternalReference xmi:id="276" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="285" namespace="GREC Concepts" ID="Descriptive-Agent"/><shared:ExternalReference xmi:id="299" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="308" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="317" namespace="GREC Concepts" ID="Manner"/><shared:ExternalReference xmi:id="329" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="342" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="351" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="364" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="373" namespace="GREC Concepts" ID="Descriptive-Theme"/><semantic:EventAnnotation xmi:id="178" sofa="1" begin="54" end="63" externalReference="186" linkedAnnotationSets="244"/><semantic:EventAnnotation xmi:id="189" sofa="1" begin="423" end="432" externalReference="197" linkedAnnotationSets="266"/><semantic:EventAnnotation xmi:id="200" sofa="1" begin="478" end="487" externalReference="208" linkedAnnotationSets="288"/><semantic:EventAnnotation xmi:id="211" sofa="1" begin="497" end="510" externalReference="219" linkedAnnotationSets="320"/><semantic:EventAnnotation xmi:id="222" sofa="1" begin="837" end="844" externalReference="230" linkedAnnotationSets="332"/><semantic:EventAnnotation xmi:id="233" sofa="1" begin="1312" end="1320" externalReference="241" linkedAnnotationSets="354"/><comparable:IDArray xmi:id="376" array="379" idType="t"/><semantic2:NucleicAcids xmi:id="37" sofa="1" begin="69" end="77" externalReference="45"/><semantic2:NucleicAcids xmi:id="59" sofa="1" begin="438" end="458" externalReference="67"/><semantic2:NucleicAcids xmi:id="77" sofa="1" begin="488" end="496" externalReference="85"/><semantic2:Proteins xmi:id="26" sofa="1" begin="0" end="18" externalReference="34"/><semantic2:Proteins xmi:id="48" sofa="1" begin="419" end="422" externalReference="56"/><semantic2:Proteins xmi:id="112" sofa="1" begin="1301" end="1304" externalReference="120"/><semantic2:Processes xmi:id="102" sofa="1" begin="849" end="867" externalReference="109"/><semantic2:EventArgumentSpan xmi:id="70" sofa="1" begin="469" end="477"/><semantic2:EventArgumentSpan xmi:id="88" sofa="1" begin="832" end="836"/><semantic2:EventArgumentSpan xmi:id="95" sofa="1" begin="845" end="876"/><semantic2:EventArgumentSpan xmi:id="123" sofa="1" begin="1324" end="1363"/><cas:View sofa="1" members="244 251 260 266 273 282 288 296 305 314 320 326 332 339 348 354 361 370 379 8 21 13 248 257 270 279 293 302 311 323 336 345 358 367 34 45 56 67 85 109 120 186 197 208 219 230 241 254 263 276 285 299 308 317 329 342 351 364 373 178 189 200 211 222 233 376 37 59 77 26 48 112 102 70 88 95 123"/></xmi:XMI>