<?xml version="1.0" encoding="UTF-8"?><xmi:XMI xmlns:pos="http:///org/u_compare/shared/label/penn/pos.ecore" xmlns:grammatical="http:///org/u_compare/shared/label/penn/function/grammatical.ecore" xmlns:general="http:///org/u_compare/shared/label/penn/pos/general.ecore" xmlns:cas="http:///uima/cas.ecore" xmlns:stanford="http:///org/u_compare/shared/label/stanford.ecore" xmlns:semantic2="http:///uk/ac/nactem/semantic.ecore" xmlns:bioie="http:///org/u_compare/shared/label/penn/pos/bioie.ecore" xmlns:function="http:///org/u_compare/shared/label/penn/function.ecore" xmlns:tcas="http:///uima/tcas.ecore" xmlns:bio="http:///org/u_compare/shared/semantic/bio.ecore" xmlns:syntactic="http:///org/u_compare/shared/syntactic.ecore" xmlns:comparable="http:///org/u_compare/shared/comparable.ecore" xmlns:examples="http:///org/apache/uima/examples.ecore" xmlns:label="http:///org/u_compare/shared/label.ecore" xmlns:xmi="http://www.omg.org/XMI" xmlns:general2="http:///org/u_compare/shared/label/penn/bracket/general.ecore" xmlns:shared="http:///org/u_compare/shared.ecore" xmlns:fragment="http:///org/u_compare/shared/document/fragment.ecore" xmlns:document="http:///org/u_compare/shared/document.ecore" xmlns:bracket="http:///org/u_compare/shared/label/penn/bracket.ecore" xmlns:text="http:///org/u_compare/shared/label/penn/function/text.ecore" xmlns:structure="http:///org/u_compare/shared/document/structure.ecore" xmlns:text2="http:///org/u_compare/shared/document/text.ecore" xmlns:release="http:///org/u_compare/shared/label/penn/pos/release.ecore" xmlns:semantic="http:///org/u_compare/shared/semantic.ecore" xmlns:nontext="http:///org/u_compare/shared/document/nontext.ecore" xmlns:conll="http:///org/u_compare/shared/label/conll.ecore" xmlns:chemical="http:///org/u_compare/shared/semantic/chemical.ecore" xmlns:semantic3="http:///org/u_compare/shared/label/penn/function/semantic.ecore" xmlns:release2="http:///org/u_compare/shared/label/penn/bracket/release.ecore" xmlns:coindex="http:///org/u_compare/shared/label/penn/coindex.ecore" xmi:version="2.0"><cas:NULL xmi:id="0"/><cas:Sofa xmi:id="1" sofaNum="1" sofaID="_InitialView" mimeType="text" sofaString="Regulated expression in vitro of genes coding for formate hydrogenlyase components of Escherichia coli. &#10;Purified FHLA, the transcriptional activator of the formate regulon from Escherichia coli, is able to efficiently stimulate transcription from the sigma 54-dependent promoters of the fdhF, hyp, and hyc transcriptional units. Expression was dependent on the presence of sigma 54, of the upstream activatory sequence (UAS), and of formate. Hypophosphite, a formate analogue, could substitute for formate in vitro suggesting that formate per se was active in regulation. The integration host factor (IHF) had a direct effect on the expression (in vivo and in vitro) of the hyp and hyc genes but not of the fdhF gene. Binding of IHF within the region between the hyp and the hyc operon could be shown. A model is proposed for the transcriptional regulation of the inversely oriented hyp and hyc operons. It involves two upstream regulatory sequences, one between the hyp and the hyc operon (IR1), and the other between hycA and hycB (IR2). The UAS situated within IR1 is responsible for activation of the hyc operon, that within IR2 for activation of the hyp operon. A supramolecular transcription complex is proposed which involves the binding of IHF to a site located between the UAS and the promoter responsible for transcription of the hyc operon. "/><cas:FSArray xmi:id="580" elements="583"/><cas:FSArray xmi:id="586" elements="426"/><cas:FSArray xmi:id="592" elements="596 605"/><cas:FSArray xmi:id="599" elements="33"/><cas:FSArray xmi:id="608" elements="26"/><cas:FSArray xmi:id="614" elements="618 627"/><cas:FSArray xmi:id="621" elements="33"/><cas:FSArray xmi:id="630" elements="44"/><cas:FSArray xmi:id="636" elements="640 649"/><cas:FSArray xmi:id="643" elements="55"/><cas:FSArray xmi:id="652" elements="459"/><cas:FSArray xmi:id="658" elements="661"/><cas:FSArray xmi:id="664" elements="66"/><cas:FSArray xmi:id="670" elements="673"/><cas:FSArray xmi:id="676" elements="77 88 99"/><cas:FSArray xmi:id="684" elements="688 697"/><cas:FSArray xmi:id="691" elements="106"/><cas:FSArray xmi:id="700" elements="492"/><cas:FSArray xmi:id="706" elements="710 720"/><cas:FSArray xmi:id="713" elements="131 142"/><cas:FSArray xmi:id="723" elements="117 124"/><cas:FSArray xmi:id="730" elements="734 743"/><cas:FSArray xmi:id="737" elements="153"/><cas:FSArray xmi:id="746" elements="164"/><cas:FSArray xmi:id="752" elements="756 765"/><cas:FSArray xmi:id="759" elements="189"/><cas:FSArray xmi:id="768" elements="182"/><cas:FSArray xmi:id="774" elements="778 787"/><cas:FSArray xmi:id="781" elements="200"/><cas:FSArray xmi:id="790" elements="207 218"/><cas:FSArray xmi:id="797" elements="801 810"/><cas:FSArray xmi:id="804" elements="229"/><cas:FSArray xmi:id="813" elements="240"/><cas:FSArray xmi:id="819" elements="823 832"/><cas:FSArray xmi:id="826" elements="251"/><cas:FSArray xmi:id="835" elements="558"/><cas:FSArray xmi:id="841" elements="845 854"/><cas:FSArray xmi:id="848" elements="262"/><cas:FSArray xmi:id="857" elements="273"/><cas:FSArray xmi:id="863" elements="866"/><cas:FSArray xmi:id="869" elements="284"/><cas:FSArray xmi:id="878" elements="0 26 33 44 55 66 77 88 99 106 117 124 131 142 153 164 171 182 189 200 207 218 229 240 251 262 273 284 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0"/><tcas:DocumentAnnotation xmi:id="8" sofa="1" begin="0" end="1353" language="x-unspecified"/><tcas:DocumentAnnotation xmi:id="21" sofa="1" begin="0" end="1353"/><examples:SourceDocumentInformation xmi:id="13" sofa="1" begin="0" end="1353" uri="/8034728.txt" offsetInSource="0" documentSize="1353" lastSegment="true"/><semantic:LinkedAnnotationSet xmi:id="583" linkingRelation="589" linkedAnnotations="586"/><semantic:LinkedAnnotationSet xmi:id="596" linkingRelation="602" linkedAnnotations="599"/><semantic:LinkedAnnotationSet xmi:id="605" linkingRelation="611" linkedAnnotations="608"/><semantic:LinkedAnnotationSet xmi:id="618" linkingRelation="624" linkedAnnotations="621"/><semantic:LinkedAnnotationSet xmi:id="627" linkingRelation="633" linkedAnnotations="630"/><semantic:LinkedAnnotationSet xmi:id="640" linkingRelation="646" linkedAnnotations="643"/><semantic:LinkedAnnotationSet xmi:id="649" linkingRelation="655" linkedAnnotations="652"/><semantic:LinkedAnnotationSet xmi:id="661" linkingRelation="667" linkedAnnotations="664"/><semantic:LinkedAnnotationSet xmi:id="673" linkingRelation="681" linkedAnnotations="676"/><semantic:LinkedAnnotationSet xmi:id="688" linkingRelation="694" linkedAnnotations="691"/><semantic:LinkedAnnotationSet xmi:id="697" linkingRelation="703" linkedAnnotations="700"/><semantic:LinkedAnnotationSet xmi:id="710" linkingRelation="717" linkedAnnotations="713"/><semantic:LinkedAnnotationSet xmi:id="720" linkingRelation="727" linkedAnnotations="723"/><semantic:LinkedAnnotationSet xmi:id="734" linkingRelation="740" linkedAnnotations="737"/><semantic:LinkedAnnotationSet xmi:id="743" linkingRelation="749" linkedAnnotations="746"/><semantic:LinkedAnnotationSet xmi:id="756" linkingRelation="762" linkedAnnotations="759"/><semantic:LinkedAnnotationSet xmi:id="765" linkingRelation="771" linkedAnnotations="768"/><semantic:LinkedAnnotationSet xmi:id="778" linkingRelation="784" linkedAnnotations="781"/><semantic:LinkedAnnotationSet xmi:id="787" linkingRelation="794" linkedAnnotations="790"/><semantic:LinkedAnnotationSet xmi:id="801" linkingRelation="807" linkedAnnotations="804"/><semantic:LinkedAnnotationSet xmi:id="810" linkingRelation="816" linkedAnnotations="813"/><semantic:LinkedAnnotationSet xmi:id="823" linkingRelation="829" linkedAnnotations="826"/><semantic:LinkedAnnotationSet xmi:id="832" linkingRelation="838" linkedAnnotations="835"/><semantic:LinkedAnnotationSet xmi:id="845" linkingRelation="851" linkedAnnotations="848"/><semantic:LinkedAnnotationSet xmi:id="854" linkingRelation="860" linkedAnnotations="857"/><semantic:LinkedAnnotationSet xmi:id="866" linkingRelation="872" linkedAnnotations="869"/><shared:ExternalReference xmi:id="41" namespace="GREC Concepts" ID="Gene"/><shared:ExternalReference xmi:id="52" namespace="GREC Concepts" ID="Sub_Unit"/><shared:ExternalReference xmi:id="63" namespace="GREC Concepts" ID="Activator"/><shared:ExternalReference xmi:id="74" namespace="GREC Concepts" ID="Promoter"/><shared:ExternalReference xmi:id="85" namespace="GREC Concepts" ID="Sigma_Factor"/><shared:ExternalReference xmi:id="96" namespace="GREC Concepts" ID="Enhancer"/><shared:ExternalReference xmi:id="114" namespace="GREC Concepts" ID="Regulator"/><shared:ExternalReference xmi:id="139" namespace="GREC Concepts" ID="Gene"/><shared:ExternalReference xmi:id="150" namespace="GREC Concepts" ID="Gene"/><shared:ExternalReference xmi:id="161" namespace="GREC Concepts" ID="Regulator"/><shared:ExternalReference xmi:id="179" namespace="GREC Concepts" ID="Locus"/><shared:ExternalReference xmi:id="197" namespace="GREC Concepts" ID="Operon"/><shared:ExternalReference xmi:id="215" namespace="GREC Concepts" ID="Transcription_Binding_Site"/><shared:ExternalReference xmi:id="226" namespace="GREC Concepts" ID="Transcription_Binding_Site"/><shared:ExternalReference xmi:id="237" namespace="GREC Concepts" ID="Enhancer"/><shared:ExternalReference xmi:id="248" namespace="GREC Concepts" ID="Operon"/><shared:ExternalReference xmi:id="259" namespace="GREC Concepts" ID="Protein_Complex"/><shared:ExternalReference xmi:id="270" namespace="GREC Concepts" ID="Regulator"/><shared:ExternalReference xmi:id="281" namespace="GREC Concepts" ID="Locus"/><shared:ExternalReference xmi:id="292" namespace="GREC Concepts" ID="Operon"/><shared:ExternalReference xmi:id="423" namespace="GREC" ID="Regulation"/><shared:ExternalReference xmi:id="434" namespace="GREC" ID="Gene_Expression"/><shared:ExternalReference xmi:id="445" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="456" namespace="GREC" ID="Gene_Activation"/><shared:ExternalReference xmi:id="467" namespace="GREC" ID="Transcription"/><shared:ExternalReference xmi:id="478" namespace="GREC" ID="Gene_Expression"/><shared:ExternalReference xmi:id="489" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="500" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="511" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="522" namespace="GREC" ID="Regulation"/><shared:ExternalReference xmi:id="533" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="544" namespace="GREC" ID="Gene_Activation"/><shared:ExternalReference xmi:id="555" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="566" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="577" namespace="GREC" ID="Transcription"/><shared:ExternalReference xmi:id="589" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="602" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="611" namespace="GREC Concepts" ID="Manner"/><shared:ExternalReference xmi:id="624" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="633" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="646" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="655" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="667" namespace="GREC Concepts" ID="Source"/><shared:ExternalReference xmi:id="681" namespace="GREC Concepts" ID="Condition"/><shared:ExternalReference xmi:id="694" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="703" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="717" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="727" namespace="GREC Concepts" ID="Manner"/><shared:ExternalReference xmi:id="740" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="749" namespace="GREC Concepts" ID="Location"/><shared:ExternalReference xmi:id="762" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="771" namespace="GREC Concepts" ID="Manner"/><shared:ExternalReference xmi:id="784" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="794" namespace="GREC Concepts" ID="Descriptive-Theme"/><shared:ExternalReference xmi:id="807" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="816" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="829" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="838" namespace="GREC Concepts" ID="Descriptive-Theme"/><shared:ExternalReference xmi:id="851" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="860" namespace="GREC Concepts" ID="Destination"/><shared:ExternalReference xmi:id="872" namespace="GREC Concepts" ID="Theme"/><semantic:EventAnnotation xmi:id="415" sofa="1" begin="0" end="9" externalReference="423" linkedAnnotationSets="580"/><semantic:EventAnnotation xmi:id="426" sofa="1" begin="10" end="20" externalReference="434" linkedAnnotationSets="592"/><semantic:EventAnnotation xmi:id="437" sofa="1" begin="39" end="45" externalReference="445" linkedAnnotationSets="614"/><semantic:EventAnnotation xmi:id="448" sofa="1" begin="219" end="228" externalReference="456" linkedAnnotationSets="636"/><semantic:EventAnnotation xmi:id="459" sofa="1" begin="229" end="242" externalReference="467" linkedAnnotationSets="658"/><semantic:EventAnnotation xmi:id="470" sofa="1" begin="330" end="340" externalReference="478" linkedAnnotationSets="670"/><semantic:EventAnnotation xmi:id="481" sofa="1" begin="620" end="626" externalReference="489" linkedAnnotationSets="684"/><semantic:EventAnnotation xmi:id="492" sofa="1" begin="634" end="644" externalReference="500" linkedAnnotationSets="706"/><semantic:EventAnnotation xmi:id="503" sofa="1" begin="719" end="726" externalReference="511" linkedAnnotationSets="730"/><semantic:EventAnnotation xmi:id="514" sofa="1" begin="847" end="857" externalReference="522" linkedAnnotationSets="752"/><semantic:EventAnnotation xmi:id="525" sofa="1" begin="908" end="916" externalReference="533" linkedAnnotationSets="774"/><semantic:EventAnnotation xmi:id="536" sofa="1" begin="1088" end="1098" externalReference="544" linkedAnnotationSets="797"/><semantic:EventAnnotation xmi:id="547" sofa="1" begin="1225" end="1233" externalReference="555" linkedAnnotationSets="819"/><semantic:EventAnnotation xmi:id="558" sofa="1" begin="1238" end="1245" externalReference="566" linkedAnnotationSets="841"/><semantic:EventAnnotation xmi:id="569" sofa="1" begin="1320" end="1333" externalReference="577" linkedAnnotationSets="863"/><comparable:IDArray xmi:id="875" array="878" idType="t"/><semantic2:NucleicAcids xmi:id="33" sofa="1" begin="33" end="38" externalReference="41"/><semantic2:NucleicAcids xmi:id="66" sofa="1" begin="248" end="280" externalReference="74"/><semantic2:NucleicAcids xmi:id="88" sofa="1" begin="421" end="424" externalReference="96"/><semantic2:NucleicAcids xmi:id="131" sofa="1" begin="675" end="692" externalReference="139"/><semantic2:NucleicAcids xmi:id="142" sofa="1" begin="708" end="717" externalReference="150"/><semantic2:NucleicAcids xmi:id="171" sofa="1" begin="741" end="751" externalReference="179"/><semantic2:NucleicAcids xmi:id="189" sofa="1" begin="861" end="903" externalReference="197"/><semantic2:NucleicAcids xmi:id="207" sofa="1" begin="992" end="995" externalReference="215"/><semantic2:NucleicAcids xmi:id="218" sofa="1" begin="1035" end="1038" externalReference="226"/><semantic2:NucleicAcids xmi:id="229" sofa="1" begin="1041" end="1048" externalReference="237"/><semantic2:NucleicAcids xmi:id="240" sofa="1" begin="1102" end="1116" externalReference="248"/><semantic2:NucleicAcids xmi:id="273" sofa="1" begin="1256" end="1262" externalReference="281"/><semantic2:NucleicAcids xmi:id="284" sofa="1" begin="1337" end="1351" externalReference="292"/><semantic2:Proteins xmi:id="44" sofa="1" begin="50" end="82" externalReference="52"/><semantic2:Proteins xmi:id="55" sofa="1" begin="105" end="118" externalReference="63"/><semantic2:Proteins xmi:id="77" sofa="1" begin="374" end="382" externalReference="85"/><semantic2:Proteins xmi:id="106" sofa="1" begin="602" end="605" externalReference="114"/><semantic2:Proteins xmi:id="153" sofa="1" begin="730" end="733" externalReference="161"/><semantic2:Proteins xmi:id="251" sofa="1" begin="1168" end="1206" externalReference="259"/><semantic2:Proteins xmi:id="262" sofa="1" begin="1249" end="1252" externalReference="270"/><semantic2:EventArgumentSpan xmi:id="26" sofa="1" begin="21" end="29"/><semantic2:EventArgumentSpan xmi:id="99" sofa="1" begin="434" end="441"/><semantic2:EventArgumentSpan xmi:id="117" sofa="1" begin="646" end="653"/><semantic2:EventArgumentSpan xmi:id="124" sofa="1" begin="658" end="666"/><semantic2:EventArgumentSpan xmi:id="164" sofa="1" begin="734" end="751"/><semantic2:EventArgumentSpan xmi:id="182" sofa="1" begin="831" end="846"/><semantic2:EventArgumentSpan xmi:id="200" sofa="1" begin="905" end="907"/><cas:View sofa="1" members="580 586 592 599 608 614 621 630 636 643 652 658 664 670 676 684 691 700 706 713 723 730 737 746 752 759 768 774 781 790 797 804 813 819 826 835 841 848 857 863 869 878 8 21 13 583 596 605 618 627 640 649 661 673 688 697 710 720 734 743 756 765 778 787 801 810 823 832 845 854 866 41 52 63 74 85 96 114 139 150 161 179 197 215 226 237 248 259 270 281 292 423 434 445 456 467 478 489 500 511 522 533 544 555 566 577 589 602 611 624 633 646 655 667 681 694 703 717 727 740 749 762 771 784 794 807 816 829 838 851 860 872 415 426 437 448 459 470 481 492 503 514 525 536 547 558 569 875 33 66 88 131 142 171 189 207 218 229 240 273 284 44 55 77 106 153 251 262 26 99 117 124 164 182 200"/></xmi:XMI>