<?xml version="1.0" encoding="UTF-8"?><xmi:XMI xmlns:pos="http:///org/u_compare/shared/label/penn/pos.ecore" xmlns:grammatical="http:///org/u_compare/shared/label/penn/function/grammatical.ecore" xmlns:general="http:///org/u_compare/shared/label/penn/pos/general.ecore" xmlns:cas="http:///uima/cas.ecore" xmlns:stanford="http:///org/u_compare/shared/label/stanford.ecore" xmlns:semantic2="http:///uk/ac/nactem/semantic.ecore" xmlns:bioie="http:///org/u_compare/shared/label/penn/pos/bioie.ecore" xmlns:function="http:///org/u_compare/shared/label/penn/function.ecore" xmlns:tcas="http:///uima/tcas.ecore" xmlns:bio="http:///org/u_compare/shared/semantic/bio.ecore" xmlns:syntactic="http:///org/u_compare/shared/syntactic.ecore" xmlns:comparable="http:///org/u_compare/shared/comparable.ecore" xmlns:examples="http:///org/apache/uima/examples.ecore" xmlns:label="http:///org/u_compare/shared/label.ecore" xmlns:xmi="http://www.omg.org/XMI" xmlns:general2="http:///org/u_compare/shared/label/penn/bracket/general.ecore" xmlns:shared="http:///org/u_compare/shared.ecore" xmlns:fragment="http:///org/u_compare/shared/document/fragment.ecore" xmlns:document="http:///org/u_compare/shared/document.ecore" xmlns:bracket="http:///org/u_compare/shared/label/penn/bracket.ecore" xmlns:text="http:///org/u_compare/shared/label/penn/function/text.ecore" xmlns:structure="http:///org/u_compare/shared/document/structure.ecore" xmlns:text2="http:///org/u_compare/shared/document/text.ecore" xmlns:release="http:///org/u_compare/shared/label/penn/pos/release.ecore" xmlns:semantic="http:///org/u_compare/shared/semantic.ecore" xmlns:nontext="http:///org/u_compare/shared/document/nontext.ecore" xmlns:conll="http:///org/u_compare/shared/label/conll.ecore" xmlns:chemical="http:///org/u_compare/shared/semantic/chemical.ecore" xmlns:semantic3="http:///org/u_compare/shared/label/penn/function/semantic.ecore" xmlns:release2="http:///org/u_compare/shared/label/penn/bracket/release.ecore" xmlns:coindex="http:///org/u_compare/shared/label/penn/coindex.ecore" xmi:version="2.0"><cas:NULL xmi:id="0"/><cas:Sofa xmi:id="1" sofaNum="1" sofaID="_InitialView" mimeType="text" sofaString="Alp suppression of Lon: dependence on the slpA gene. &#10;We have previously found that plasmids carrying the Escherichia coli alp gene (now to be called alpA) suppress two phenotypes of a delta lon protease mutant, overproduction of capsular polysaccharide and sensitivity to UV light. Suppression of these lon phenotypes is most likely explained by the increased degradation of the Lon substrates responsible for these phenotypes. We have called this suppressing protease activity Alp protease. The Alp protease activity is detected in cells after introduction of plasmids carrying the alpA gene, which encodes an open reading frame of 70 amino acids. Insertions which abolish Alp activity interrupt this open reading frame. We have used Tn10 and lambda placMu mutagenesis to identify a chromosomal locus, slpA, that is required for alpA+ suppression of delta lon. This locus maps at 57 min, close to the chromosomal location of alpA. The expression of beta-galactosidase from a lac transcriptional fusion to slpA is increased six- to eightfold when the alpA+ gene is present on a multicopy plasmid. Therefore, AlpA acts as a transcriptional regulator of the slpA gene(s); activation of slpA transcription is necessary to suppress the phenotypes of a delta lon mutation. In an accompanying paper (J.E. Kirby, J.E. Trempy, and S. Gottesman, J. Bacteriol. 176:2068-2081, 1994), we show that neither AlpA nor SlpA is a component of the protease itself but that they are part of a regulatory cascade which leads to expression of the Alp protease. "/><cas:FSArray xmi:id="461" elements="465 474"/><cas:FSArray xmi:id="468" elements="26"/><cas:FSArray xmi:id="477" elements="37"/><cas:FSArray xmi:id="483" elements="487 496"/><cas:FSArray xmi:id="490" elements="48"/><cas:FSArray xmi:id="499" elements="59"/><cas:FSArray xmi:id="505" elements="509 518"/><cas:FSArray xmi:id="512" elements="70"/><cas:FSArray xmi:id="521" elements="80"/><cas:FSArray xmi:id="527" elements="531 540"/><cas:FSArray xmi:id="534" elements="94"/><cas:FSArray xmi:id="543" elements="87"/><cas:FSArray xmi:id="549" elements="553 562"/><cas:FSArray xmi:id="556" elements="94"/><cas:FSArray xmi:id="565" elements="112"/><cas:FSArray xmi:id="571" elements="575 584"/><cas:FSArray xmi:id="578" elements="105"/><cas:FSArray xmi:id="587" elements="119"/><cas:FSArray xmi:id="593" elements="597 606"/><cas:FSArray xmi:id="600" elements="137"/><cas:FSArray xmi:id="609" elements="148"/><cas:FSArray xmi:id="615" elements="619 628"/><cas:FSArray xmi:id="622" elements="395"/><cas:FSArray xmi:id="631" elements="166"/><cas:FSArray xmi:id="637" elements="640"/><cas:FSArray xmi:id="643" elements="191"/><cas:FSArray xmi:id="649" elements="652"/><cas:FSArray xmi:id="655" elements="180"/><cas:FSArray xmi:id="661" elements="665 674"/><cas:FSArray xmi:id="668" elements="173"/><cas:FSArray xmi:id="677" elements="198"/><cas:FSArray xmi:id="683" elements="687 696"/><cas:FSArray xmi:id="690" elements="215"/><cas:FSArray xmi:id="699" elements="222"/><cas:FSArray xmi:id="708" elements="0 26 37 48 59 70 80 87 94 105 112 119 126 137 148 155 166 173 180 191 198 205 215 222 0 0 0 0 0 0 0 0 0 0 0 0"/><tcas:DocumentAnnotation xmi:id="8" sofa="1" begin="0" end="1541" language="x-unspecified"/><tcas:DocumentAnnotation xmi:id="21" sofa="1" begin="0" end="1541"/><examples:SourceDocumentInformation xmi:id="13" sofa="1" begin="0" end="1541" uri="/7511582.txt" offsetInSource="0" documentSize="1541" lastSegment="true"/><semantic:LinkedAnnotationSet xmi:id="465" linkingRelation="471" linkedAnnotations="468"/><semantic:LinkedAnnotationSet xmi:id="474" linkingRelation="480" linkedAnnotations="477"/><semantic:LinkedAnnotationSet xmi:id="487" linkingRelation="493" linkedAnnotations="490"/><semantic:LinkedAnnotationSet xmi:id="496" linkingRelation="502" linkedAnnotations="499"/><semantic:LinkedAnnotationSet xmi:id="509" linkingRelation="515" linkedAnnotations="512"/><semantic:LinkedAnnotationSet xmi:id="518" linkingRelation="524" linkedAnnotations="521"/><semantic:LinkedAnnotationSet xmi:id="531" linkingRelation="537" linkedAnnotations="534"/><semantic:LinkedAnnotationSet xmi:id="540" linkingRelation="546" linkedAnnotations="543"/><semantic:LinkedAnnotationSet xmi:id="553" linkingRelation="559" linkedAnnotations="556"/><semantic:LinkedAnnotationSet xmi:id="562" linkingRelation="568" linkedAnnotations="565"/><semantic:LinkedAnnotationSet xmi:id="575" linkingRelation="581" linkedAnnotations="578"/><semantic:LinkedAnnotationSet xmi:id="584" linkingRelation="590" linkedAnnotations="587"/><semantic:LinkedAnnotationSet xmi:id="597" linkingRelation="603" linkedAnnotations="600"/><semantic:LinkedAnnotationSet xmi:id="606" linkingRelation="612" linkedAnnotations="609"/><semantic:LinkedAnnotationSet xmi:id="619" linkingRelation="625" linkedAnnotations="622"/><semantic:LinkedAnnotationSet xmi:id="628" linkingRelation="634" linkedAnnotations="631"/><semantic:LinkedAnnotationSet xmi:id="640" linkingRelation="646" linkedAnnotations="643"/><semantic:LinkedAnnotationSet xmi:id="652" linkingRelation="658" linkedAnnotations="655"/><semantic:LinkedAnnotationSet xmi:id="665" linkingRelation="671" linkedAnnotations="668"/><semantic:LinkedAnnotationSet xmi:id="674" linkingRelation="680" linkedAnnotations="677"/><semantic:LinkedAnnotationSet xmi:id="687" linkingRelation="693" linkedAnnotations="690"/><semantic:LinkedAnnotationSet xmi:id="696" linkingRelation="702" linkedAnnotations="699"/><shared:ExternalReference xmi:id="34" namespace="GREC Concepts" ID="Repressor"/><shared:ExternalReference xmi:id="45" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="56" namespace="GREC Concepts" ID="Gene"/><shared:ExternalReference xmi:id="67" namespace="GREC Concepts" ID="ORF"/><shared:ExternalReference xmi:id="77" namespace="GREC Concepts" ID="Mutation"/><shared:ExternalReference xmi:id="102" namespace="GREC Concepts" ID="Locus"/><shared:ExternalReference xmi:id="134" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="145" namespace="GREC Concepts" ID="Enzyme"/><shared:ExternalReference xmi:id="163" namespace="GREC Concepts" ID="Locus"/><shared:ExternalReference xmi:id="188" namespace="GREC Concepts" ID="Locus"/><shared:ExternalReference xmi:id="212" namespace="GREC Concepts" ID="Mutation"/><shared:ExternalReference xmi:id="230" namespace="GREC Concepts" ID="Protein"/><shared:ExternalReference xmi:id="337" namespace="GREC" ID="Gene_Repression"/><shared:ExternalReference xmi:id="348" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="359" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="370" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="381" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="392" namespace="GREC" ID="Gene_Repression"/><shared:ExternalReference xmi:id="403" namespace="GREC" ID="Gene_Expression"/><shared:ExternalReference xmi:id="414" namespace="GREC" ID="GRE"/><shared:ExternalReference xmi:id="425" namespace="GREC" ID="Gene_Activation"/><shared:ExternalReference xmi:id="436" namespace="GREC" ID="Transcription"/><shared:ExternalReference xmi:id="447" namespace="GREC" ID="Gene_Repression"/><shared:ExternalReference xmi:id="458" namespace="GREC" ID="Gene_Expression"/><shared:ExternalReference xmi:id="471" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="480" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="493" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="502" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="515" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="524" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="537" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="546" namespace="GREC Concepts" ID="Instrument"/><shared:ExternalReference xmi:id="559" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="568" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="581" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="590" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="603" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="612" namespace="GREC Concepts" ID="Source"/><shared:ExternalReference xmi:id="625" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="634" namespace="GREC Concepts" ID="Rate"/><shared:ExternalReference xmi:id="646" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="658" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="671" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="680" namespace="GREC Concepts" ID="Theme"/><shared:ExternalReference xmi:id="693" namespace="GREC Concepts" ID="Agent"/><shared:ExternalReference xmi:id="702" namespace="GREC Concepts" ID="Theme"/><semantic:EventAnnotation xmi:id="329" sofa="1" begin="4" end="15" externalReference="337" linkedAnnotationSets="461"/><semantic:EventAnnotation xmi:id="340" sofa="1" begin="601" end="608" externalReference="348" linkedAnnotationSets="483"/><semantic:EventAnnotation xmi:id="351" sofa="1" begin="667" end="674" externalReference="359" linkedAnnotationSets="505"/><semantic:EventAnnotation xmi:id="362" sofa="1" begin="774" end="782" externalReference="370" linkedAnnotationSets="527"/><semantic:EventAnnotation xmi:id="373" sofa="1" begin="818" end="826" externalReference="381" linkedAnnotationSets="549"/><semantic:EventAnnotation xmi:id="384" sofa="1" begin="837" end="848" externalReference="392" linkedAnnotationSets="571"/><semantic:EventAnnotation xmi:id="395" sofa="1" begin="937" end="947" externalReference="403" linkedAnnotationSets="593"/><semantic:EventAnnotation xmi:id="406" sofa="1" begin="1015" end="1024" externalReference="414" linkedAnnotationSets="615"/><semantic:EventAnnotation xmi:id="417" sofa="1" begin="1171" end="1181" externalReference="425" linkedAnnotationSets="637"/><semantic:EventAnnotation xmi:id="428" sofa="1" begin="1190" end="1203" externalReference="436" linkedAnnotationSets="649"/><semantic:EventAnnotation xmi:id="439" sofa="1" begin="1220" end="1228" externalReference="447" linkedAnnotationSets="661"/><semantic:EventAnnotation xmi:id="450" sofa="1" begin="1509" end="1519" externalReference="458" linkedAnnotationSets="683"/><comparable:IDArray xmi:id="705" array="708" idType="t"/><semantic2:NucleicAcids xmi:id="48" sofa="1" begin="580" end="593" externalReference="56"/><semantic2:NucleicAcids xmi:id="59" sofa="1" begin="609" end="630" externalReference="67"/><semantic2:NucleicAcids xmi:id="94" sofa="1" begin="804" end="808" externalReference="102"/><semantic2:NucleicAcids xmi:id="155" sofa="1" begin="1007" end="1011" externalReference="163"/><semantic2:NucleicAcids xmi:id="180" sofa="1" begin="1185" end="1189" externalReference="188"/><semantic2:Proteins xmi:id="26" sofa="1" begin="0" end="3" externalReference="34"/><semantic2:Proteins xmi:id="37" sofa="1" begin="19" end="22" externalReference="45"/><semantic2:Proteins xmi:id="126" sofa="1" begin="863" end="873" externalReference="134"/><semantic2:Proteins xmi:id="137" sofa="1" begin="951" end="969" externalReference="145"/><semantic2:Proteins xmi:id="222" sofa="1" begin="1523" end="1539" externalReference="230"/><semantic2:Processes xmi:id="70" sofa="1" begin="650" end="660" externalReference="77"/><semantic2:Processes xmi:id="205" sofa="1" begin="1247" end="1267" externalReference="212"/><semantic2:EventArgumentSpan xmi:id="80" sofa="1" begin="675" end="687"/><semantic2:EventArgumentSpan xmi:id="87" sofa="1" begin="736" end="770"/><semantic2:EventArgumentSpan xmi:id="105" sofa="1" begin="831" end="836"/><semantic2:EventArgumentSpan xmi:id="112" sofa="1" begin="831" end="848"/><semantic2:EventArgumentSpan xmi:id="119" sofa="1" begin="852" end="861"/><semantic2:EventArgumentSpan xmi:id="148" sofa="1" begin="975" end="1011"/><semantic2:EventArgumentSpan xmi:id="166" sofa="1" begin="1025" end="1042"/><semantic2:EventArgumentSpan xmi:id="173" sofa="1" begin="1171" end="1203"/><semantic2:EventArgumentSpan xmi:id="191" sofa="1" begin="1185" end="1203"/><semantic2:EventArgumentSpan xmi:id="198" sofa="1" begin="1229" end="1267"/><semantic2:EventArgumentSpan xmi:id="215" sofa="1" begin="1473" end="1493"/><cas:View sofa="1" members="461 468 477 483 490 499 505 512 521 527 534 543 549 556 565 571 578 587 593 600 609 615 622 631 637 643 649 655 661 668 677 683 690 699 708 8 21 13 465 474 487 496 509 518 531 540 553 562 575 584 597 606 619 628 640 652 665 674 687 696 34 45 56 67 77 102 134 145 163 188 212 230 337 348 359 370 381 392 403 414 425 436 447 458 471 480 493 502 515 524 537 546 559 568 581 590 603 612 625 634 646 658 671 680 693 702 329 340 351 362 373 384 395 406 417 428 439 450 705 48 59 94 155 180 26 37 126 137 222 70 205 80 87 105 112 119 148 166 173 191 198 215"/></xmi:XMI>