Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q9UIJ5 (Rec)
 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mutagenicity screening was carried out on 31 samples of popular Thai spices derived from 12 different families of plants, namely the Amaryllidaceae (2), Graminae (1), Labiatae (4), Lauraceae (1), Magnoliaceae (1), Myristicaceae (2), Myrtaceae (2), Piperaceae (3), Rutaceae (2), Solanaceae (2), Umbelliferae (2) and Zingiberaceae (9). Two variations of the rapid streak method of rec-assay in Bacillus subtilis strains H17 (rec+) and M45 (rec-) were used. Only Ceylon cinnamon (the bark of Cinnamomum zeylanicum Nees of the family Lauraceae) showed mutagenic activity. The crude form of this spice and its water-heated and water-macerated residues all produced the rec effect, while water-heated and water-macerated filtrates did not, even in concentrations equivalent to as much as 50 mg solids/test disc.
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PMID:Mutagenicity screening of popular Thai spices. 681 37

The rec-assay with Bacillus subtilis strains H17 Rec+ and M45 Rec- was carried out with their spores. The use of spores ensured more than 50 times higher sensitivities with several typical chemical mutagens compared with those with vegetative cells. This improved sensitivity enabled us to detect DNA-damaging activities of typical metabolically activated chemicals. Conditions of improvements and quantitative aspects are analyzed and described.
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PMID:rec-Assay with spores of Bacillus subtilis with and without metabolic activation. 681 26

We attempted to detect mutagenic activity in bile and pancreatic juice from patients with biliary tract disease using the spore rec assay and wild (H17) and mutant (M45) strains. Three bile samples out of 5 obtained from patients with pancreatico-biliary maljunction showed positive reaction in the spore rec assay, and all contained a high level of amylase activity, while 300 microliters of bile samples obtained from 10 control patients without pancreatico-biliary maljunction did not show any positive reaction. Moreover, 300 microliters of the in vitro mixture of bile with an equal volume of pancreatic juice also showed a positive reaction after treatment for 12 days at 37 degrees C or for 10 min at 100 degrees C, suggesting that they were very stable and long-acting in vivo. These data suggest that possible mutagens might be formed by the mixing of bile with pancreatic juice regurgitated into the biliary tract, and that there might be a relationship to biliary tract cancer which often accompanies pancreatico-biliary maljunction.
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PMID:Mutagenicity of bile and pancreatic juice from patients with pancreatico-biliary maljunction. 754 38

A phytotoxic substance (C23H44O3) which is named 'Substance A', was purified from olive leaves infected with Cycloconium oleaginum Cast. The mutagenic effect of this substance was detected using TA 100 and TA 102 strains of Salmonella in the Ames test using Bacillus subtilis strains M45 rec-, H17 rec+ in the rec assay. Another substance manifesting the mutagenic effect was found in the extract from the Cycloconium oleaginum culture. This substance was not detected in the extract from contaminated olive leaves. Substance A increased electrolytes leakage from tissue of olive leaves, thus manifesting its phytotoxicity.
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PMID:Mutagenic and membranal effect of a phytotoxic molecule isolated from olive leaves parasitized by the fungus Cycloconium oleaginum Cast. 806 32

Five synthetic food colours Food Red Nos 3, 40 and 102 and Food Blue Nos 1 and 2, and their UV irradiated products were tested for mutagenic activity by means of the Ames test using Salmonella typhimurium strains TA98 and TA100. Food colours were irradiated with UV light for 14 days. Food Red Nos 3, 40 and 102 and Food Blue No. 1 were non-mutagenic before and after irradiation. UV irradiated products of Food Blue No. 2 were mutagenic in TA98 with or without S-9 mix. The mutagenic activity increased with increasing irradiation period, reached maximum potency on day 6, and then decreased. Moreover, Food Blue No. 2 showed DNA-damaging activity after 14 days of irradiation in rec-assay using Bacillus subtilis strains H17 and M45. The capillary electrophoresis was applied for the analysis of UV irradiated products of Food Blue No. 2. The original peak of Food Blue No. 2 was decomposed into seven peaks after UV irradiation.
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PMID:Mutagenicity and DNA-damaging activity of decomposed products of food colours under UV irradiation. 973 28

A simple and rapid method for spore rec-assay by utilizing dry sheet medium culture (Compactdry TC, CTC) for determining numbers of bacteria, instead of the spore agar plate, was developed. One mL of spore suspension (2 x 10(6)/mL) of Bacillus subtilis strain M45 Rec- or H17 Rec+ was inoculated in the center of the CTC plate. In the case of metabolic activation, 1 mL of mixed solution (spore suspension of M45 or H17: 9,000 x g supernatant of rat-liver homogenate treated with Aroclor 1254 = 19:1) was used. The spore suspension spreads over the whole sheet in seconds and gels. A paper disk impregnated with 20-40 microL of the sample solution and 20 microL of the cofactor solution was placed on the surface of CTC plate. For the assay of samples that do not require metabolic activation, use of the cofactor solution can be omitted. After 48 hr incubation at 37 degrees C, 0.01% MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] aqueous solution (0.5 mL) was dropped uniformly on the plate. The plate was left for 5 min, and the diameter of the inhibition circle was measured with slide calipers. The samples for which the difference in inhibition zone between M45 and H17 was more than 2 mm were judged positive. Under these conditions, the DNA damaging activities of sodium sulfite, sodium benzoate and citric acid, used as food additives, were investigated by the proposed method. Sodium sulfite and sodium benzoate gave positive results and citric acid gave a negative result with or without metabolic activation, in agreement with the results obtained by the conventional method.
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PMID:[Spore rec-assay by dry sheet medium culture plate for bacterial counts]. 1199 19

The results of four toxicity bioassays of selected anionic and nonionic surface active agents were presented. Three widely used anionic surfactants that belong to alkyl sulphates (AS), alkylbenzene sulphonates (LAS) and alkylpolyoxyethylene sulphates (AES) as well as nonionic surfactants: polyoxyethylene alkyl ethers (AE) and polyoxylethylene alkylphenyl ethers (APE) were tested. Three different toxicity assays to aquatic organisms: Physa acuta Draparnaud, Artemia salina and Raphidocelis subcapitata were applied. Additionally, the genotoxicity test with Bacillus subtilis M45 Rec- and H17 Rec+ strains was performed. The obtained results showed that none of the surfactants studied was genotoxic at the concentration 1000 mg l(-1). On the basis of toxicity tests to aquatic organisms all tested anionic surfactants were harmful (LC50 between 10 and 100 mg l(-1)), whereas nonionic ones were toxic (LC50 between 1 and 10 mg l(-1)) or even highly toxic (LC50 below 1 mg l(-1)). Moreover, the bigger was the molecular weight of the tested compound, the higher toxicity was observed.
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PMID:Acute toxicity and genotoxicity of five selected anionic and nonionic surfactants. 1566 44

Further to a previous report (Food Chem. Toxicol. 22:109-112, 1984) describing the presence of mutagens in a chloroform extract of Ceylon cinnamon ( Cinnamomum zeylanicum Nees), we report here our findings on the mutagenicity of silica gel column chromatographic fractions from this extract. Mutagenicity was evaluated by the rec assay using Bacillus subtillis strains H17 (rec +) and M45 (rec-). Fractions collected after elution with chloroform, chloroform-ethanol (98:2) and (95:5) exhibited mutagenicity, while those obtained by ellution with chloroform-ethanol (85:15), (75:25) and ethanol showed no such activity.
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PMID:Bacterial Mutagenicity of Fractions from Chloroform Extracts of Ceylon Cinnamon. 3095 4


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