Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:Q9UIJ5 (Rec)
 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Two new PCR-based methods were developed to decode prion protein (PrP) gene polymorphisms at codons 136, 154 and 171: a PCR-restriction fragment length polymorphism (RFLP) analysis consisting of two PCR reactions followed by three enzymatic digestions, and a real-time PCR consisting of four reactions with seven fluorogenic probes. Both methods were used to study the distribution of PrP gene polymorphisms in a representative sample (1297 animals) of the populations of the two native breeds of sheep of the Spanish Basque Country, Latxa and Carranzana. Fourteen genotypes were found in the Latxa breed, in which ARQ/ARQ was the genotype most frequently observed (49.3 per cent), followed by ARR/ARQ (32.6 per cent) and ARQ/ARH (5.8 per cent). The genotype associated with the highest resistance to scrapie (ARR/ARR) was present in 5 per cent of the animals analysed. Similar results were observed in the Carranzana sheep.
Vet Rec 2004 Jun 05
PMID:PrP polymorphisms in Basque sheep breeds determined by PCR-restriction fragment length polymorphism and real-time PCR. 1521 15

In total 31,669 blood samples were collected from 1187 flocks of 27 rare breeds of sheep in the UK, and their genotype profiles at the prion protein locus were determined. The frequencies of the five alleles varied widely among the breeds and some had only two of the alleles and others had all five; the average was three. The average allele frequencies across all 27 breeds were 49.7 per cent for ARR, 4.4 per cent for AHQ, 2.7 per cent for ARH, 37.4 per cent for ARQ and 5.8 per cent for VRQ. The highest frequencies for each allele were 90.7 per cent for ARR in the Leicester Longwool, 24.7 per cent for AHQ in the Hebridean, 68.7 per cent for ARH in the Manx Loghtan, 98.7 per cent for ARQ in the North Ronaldsay and 28.4 per cent for VRQ in the Boreray. All 27 breeds had the ARR allele, 21 had AHQ, 11 had ARH, 26 had ARQ and 20 had VRQ.
Vet Rec 2005 Jan 29
PMID:PrP genotypes of rare breeds of sheep in Great Britain. 1571 3

Between October 2001 and January 2003 the prion protein (PrP) genotypes of over 250,000 sheep were determined through the operation of the National Scrapie Plan (NSP); the results for 38 breeds were analysed to provide an estimate of the underlying PrP genotype distribution of the British sheep population. Although there was marked variability among the genotype profiles of the different breeds, several trends emerged. A comparison of the allele frequencies demonstrated that the breeds could be grouped into three categories: breeds dominated by ARR and ARQ in which the frequency of ARR exceeded the frequency of ARQ; breeds dominated by ARR and ARQ in which the frequency of ARQ exceeded the frequency of ARR; and breeds with significant levels of either AHQ, ARH or VRQ. Hill breeds were more likely to have a lower proportion of animals at low risk of scrapie (NSP type 1) and a higher proportion of animals at an intermediate risk of scrapie (NSP type 3) than other breeds. Most breeds had a small proportion of animals at high risk of scrapie (NSP type 5). The frequency of ARR/VRQ (NSP type 4) was variable.
Vet Rec 2005 Apr 02
PMID:Frequencies of PrP genotypes in 38 breeds of sheep sampled in the National Scrapie Plan for Great Britain. 1582 23

Prion protein (PrP) genotype data from statutory confirmed cases and from three non-case datasets have been used to calculate the odds ratio (or) for the development of clinical scrapie for an individual sheep of a given PrP genotype, compared with one possessing the "wild-type" ARQ/ARQ genotype. Logistic regression has been used to estimate the ors, and a multiple-test procedure has been used to evaluate the statistical significance of each comparison. The results are similar to those observed in other studies: the VRQ/VRQ genotype has or point estimates greater than 20; the ARQ/VRQ and ARH/VRQ genotypes have or point estimates between 5 and 20; AHQ/VRQ between 0.03 and 0.1; ARR/VRQ 0.4 and 0.5; all the other PrP genotypes, excluding ARR/ARR, ARR/ARH and AHQ/ARH for which no clinical cases have been recorded have or point estimates of less than 0.3. The estimates derived from each dataset are comparable, but not identical. This can be explained by plausible biases inherent in the sampling of the non-case populations.
Vet Rec 2006 Jan 14
PMID:Estimation of the relative risk of developing clinical scrapie: the role of prion protein (PrP) genotype and selection bias. 1641 31

In order to determine the PRNP genotype distribution of the sheep population and to assess the impact of the National Scrapie Plan, a total of 9972 brain samples were collected between January 2012 and April 2013 from sheep sampled under the TSE active surveillance programme in Great Britain. Genotype frequencies observed were ARR/ARQ (29.5 per cent), ARR/ARR (28.6 per cent), ARR/AHQ (10.9 per cent), ARQ/ARQ (9.8 per cent) and AHQ/ARQ (7.2 per cent). Of the remaining possible genotypes, none accounted for more than 5 per cent of the sheep sampled. ARR was the most frequent allele (52.3 per cent), followed by the wild-type ARQ (30 per cent), AHQ (11.3 per cent), VRQ (3.4 per cent) and ARH (3 per cent). There has been a positive but limited effect of the breeding for resistance schemes within Great Britain on the genotype distribution in the sheep national flock. The dissemination of the resistant alleles has proven to have been less noticeable than in the breeding flocks. The risk of atypical scrapie remains very similar to that in 2002. Significant differences were observed in the genotype profiles of the abattoir survey and fallen stock survey samples. It would be advisable to promote best practice among farmers to consolidate the level of genetic resistance in their flocks and to set up a national flock genotype monitoring programme in partnership with the industry.
Vet Rec 2014 May 24
PMID:The impact of the National Scrapie Plan on the PRNP genotype distribution of the British national flock, 2002-2012. 2469 42