Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:Q9UIJ5 (
Rec
)
58,342
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Antiproliferative activity of recombinant murine interferon-beta (Rec-MuIFN-beta) combined with hyperthermia against Rous sarcoma virus-induced mouse malignant glioma (RSV glioma) was investigated both in vitro and in vivo. In vitro, the antiproliferative activity of
Rec
-MuIFN-beta was enhanced by incubation at elevated temperatures (40 degrees and 43 degrees C). In vivo, combined therapy of
Rec
-MuIFN-beta treatment and local tumour hyperthermia (43 degrees C) exerted a greater antitumour effect against transplanted RSV glioma in C3H/He mice than either treatment alone, especially when
Rec
-MuIFN-beta was administered intratumourally. Subsequently, in order to probe the mechanism of enhanced antiproliferative activity, the production of prostaglandin E2 (PGE2) and 2', 5'-
oligoadenylate synthetase
(
2-5A synthetase
) in the culture medium of RSV glioma cells was measured.
Rec
-MuIFN-beta treatment resulted in a significantly greater PGE2 and
2-5A synthetase
production at 43 degrees C than at 37 degrees C.
...
PMID:Antitumour effect of interferon combined with hyperthermia against experimental brain tumour. 348 Mar 23
Human placental trophoblasts produce interferon (tro-IFNs) when stimulated with viral inducers. Since the antiviral and cellular functions of IFNs are partly mediated by the 2',5'-oligoadenylate synthetase (
2-5A synthetase
) pathway, the aim of the present study was to determine the basal and IFN-induced levels of
2-5A synthetase
in villous trophoblast cultures. A considerable basal level of
2-5A synthetase
was observed in syncytiotrophoblast cultures from both first and third trimester. In contrast no basal activity was detectable in placental fibroblast- and trophoblast-derived malignant cell lines (Far, FEG-3, and BeWo). Stimulation with tro-IFN-beta, -alpha and leucocyte-IFN (leu-IFN)-alpha increased the enzyme activity in first and third trimester human syncytiotrophoblast cultures. Treatment with recombinant-IFN (rec-IFN)-gamma significantly enhanced
2-5A synthetase
activity in first trimester syncytiotrophoblast, but had no effect on third trimester syncytiotrophoblast. Tro-IFN-beta, -alpha and leu-IFN-alpha induced high levels of
2-5A synthetase
activity in placental fibroblast, BeWo and FEG-3 cell-lines, whereas
rec
-IFN-gamma treatment did not induce
2-5A synthetase
activity in any of these cells. No detectable
2-5A synthetase
activity was found in the Far cell line. The capability of cells deriving from the fetoplacental unit to raise an antiviral response by the induction of
2-5A synthetase
may be important in defending the fetus against viral infection from the mother. Furthermore
2-5A synthetase
in cells of the fetoplacental unit may play a role in their normal growth and development.
...
PMID:Basal and interferon-induced 2',5'-oligoadenylate synthetase activity in human placental trophoblast and trophoblast-derived malignant cell lines. 754 Jul 57
