Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q9UIJ5 (Rec)
 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A series of rec-Escherichia coli strains were tested for their sensitivity to four known carcinogenic compounds by examination of a zone of inhibited bacterial growth around a central well containing the test chemical. The mutants recA-, recB-, recC-, and recA- recB- recC- were all more sensitive to the mutagens than the parental strain AB1157. The recB- recC- strain was examined with a larger series of compounds and was found to respond to many of the substances in a similar way as the Salmonella typhimurium strains of Ames but with some notable exceptions. Nitrosamines, with rat liver microsomal activation, could be detected at lower levels and a group of aromatic amino compounds failed to react with these rec-E. coli. An unusual feature of these rec-mutants is their sensitivity to mixtures of nitrosamines and 2-acetyl amino-fluorene in the absence of microsomal activation.
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PMID:The use of rec-bacteria for testing of carcinogenic substances. 32 3

Cytochemical and biochemical glucose 6-phosphatase (G6Pase) activity and fiber type composition were studied in soleus (SOL) and gastrocnemius (GC) muscles of mice. The SOL is a red muscle which contains numerous type I fibers (60%) and relatively few type II fibers (40%). The GC is a white muscle which contains numerous type II fibers (90-100%) and very few type I fibers (0-10%). In the SOL and GC, cytochemical G6Pase activity was localized in the sarcoplasmic reticulum, lateral elements of triads, myonuclear envelope, and in the endoplasmic reticulum and nuclear envelope of endothelial cells. Differential centrifugation showed that G6Pase activity was recovered in the 105,000g pellet (microsomal fraction). Histochemical enzyme activity in type II fibers was slightly higher than that in type I fibers. Biochemical G6Pase activity in the GC was significantly higher than that in the SOL. The possible functional significance of G6Pase in skeletal muscles was discussed.
Anat Rec 1986 Jan
PMID:Cytochemical and biochemical glucose 6-phosphatase activity in skeletal muscle cells of mice. 300 47

In this report we quantitated ultrastructural changes in two cytologically distinct secretory cell populations from the rabbit endocervix. Type I and type II cells from estrous animals differ only in the presence of one or more empty cytoplasmic vacuoles in type II cells. Comparing type II cells from 5-day pseudopregnant (PSP) rabbits with type II cells from estrous controls, there is no increase (P greater than .05) in the average vacuole volume. When type I and type II cells from PSP animals are compared to cells from estrous controls, there is a decrease (P less than .01) in the average cell volume, a decrease (P less than .01) in the average nuclear volume, and a decrease (P less than .01) in the average granule volume. This reduction in the granule content of secretory endocervical cells was correlated with a dramatic decrease in protein glycosylation into the microsomal fraction. Serum estradiol concentrations for estrous (13.7 +/- 1.0 pg/ml) and PSP (18.1 +/- 1.5 pg/ml) animals were comparable. However, the 36-fold increase in serum progesterone concentrations for PSP (12.04 +/- 1.7 ng/ml) animals compared to estrous (0.33 +/- 0.1 ng/ml) animals may be responsible for the decrease in protein glycosylation.
Anat Rec 1986 Dec
PMID:Rabbit endocervical epithelium: morphometric analysis of secretory cell populations. 379 98

The activities of 2,4,5,7-tetraiodofluorescein, disodium salt (erythrosine) and 2 phloxine dyes (2,4,5,7-tetrabromo-12,15-dichlorofluorescein, dipotassium salt and the disodium salt of 2,4,5,7-tetraiodo-12,15-dichlorofluorescein) have been determined using DNA-repair, fluctuation and treat-and-plate assays. Tests were conducted with and without illumination from a daylight fluorescent lamp. Both phloxine dyes were active in a rec assay but only in the absence of a rat-liver microsomal metabolising system. Erythrosine was inactive under all conditions. Although the results agreed with some of the published data for these foods and cosmetic colours, previous reports of photodynamic activation and mutagenicity were not confirmed. In the light of recent concern over the efficacy of bacterial DNA-repair tests, it is considered that the results obtained are not at present conclusive evidence for genotoxic hazard of any of the dyes studied.
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PMID:Studies on the genotoxicity of some fluorescein dyes. 625 21

Hexachloroacetone, CCl3--CO--CCl3, reverts the Ames strains TA98 and TA100 but not the non-plasmid strains TA1537, TA1535 and TA1538. In the absence of solvent, the number of revertant colonies is 5 times the spontaneous reversion rate for TA100 and 10 times the spontaneous reversion rate for TA98 with 26 mg hexachloroacetone per plate. This effect is seen in the absence of rat liver microsomes. In dimethylsulfoxide (DMSO) solution a more complicated pattern is seen. In DMSO solution cooled between 18 and 20 degrees C, The maximum nuber of revertants is similar to that found in the absence of DMSO, but only 1.75 mg hexachloroacetone per plate is needed. When DMSO solution of hexachloroacetone is warmed above 20 degrees C, a yellow color develops and the solution becomes more toxic to the test bacteria. The maximum number of revertants is then produced at about 0.5 mg hexachloroacetone per plate. Hexachloroacetone is found to be active, without microsomal activation, in the E. coli WP-2 and E. coli rec-BC test systems. Hexachloroacetone readily reacts with water in DMSO solutions to form the non-mutagenic hexachloroacetone hydrate.
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PMID:The mutagenic properties of hexachloroacetone in short-term bacterial mutagen assay systems. 702 27

alpha-(2,4-Dichlorophenyl)-beta,N-imidazolylethyl 4-phenylthiobenzyl ether nitrate (fenticonazole, Rec 15/1476), a new potent antibacterial and antifungal imidazole derivative, was tested for mutagenicity in the Salmonella reversion assay developed by Ames et al. Fenticonazole was found to be negative for strains TA 1535, TA 1537, TA 1538, TA 98, TA 100 with and without microsomal activation.
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PMID:The Salmonella mutagenicity assay on fenticonazole, a new antifungal imidazole derivative. 703 10

The effects of Nafenopin, a hypolipidemic drug, on the zona fasciculata of the rat adrenal cortex were investigated by biochemical, stereological, and cytochemical methods. Chronic Nafenopin treatment (5 days) significantly lowered serum cholesterol level, while it did not alter blood corticosterone concentration and 11beta-hydroxylase activity of adrenocortical cells. Stereology showed a significant increase in the average volume of zona fasciculata cells, which was almost exclusively due to smooth endoplasmic reticulum (SER) proliferation. The volume of lipid compartment was significantly reduced, whereas the volume of diaminobenzidine (DAB)-positive bodies (peroxisomes) per cell displayed a marked increase. Cholesterol administration per os to the treated animals raised the serum cholesterol level and completely reversed the Nafenopin effects. One day of Nafenopin administration provoked a slight but significant lipid depletion in adrenocortical cells, while 3 days of continuous drug treatment induced an extreme lipid depletion and a moderate increase in the SER coupled with a significant decrease in the plasma concentration of corticosterone. Since microsomal fraction and catalase seem to be involved in the cholesterol metabolism and utilization, the hypothesis is advanced that the Nafenopin-elicited SER and peroxisome proliferation is a compensatory response enabling adrenocortical cells to maintain an adequate level of hormonal output.
Anat Rec 1982 Nov
PMID:Effects of the hypolipidemic drug nafenopin on the zona fasciculata of the rat adrenal cortex: a correlated biochemical and stereological study. 715 28

The Bacillus subtilis 1791 rec- assay was used to quantify genotoxic mycotoxins. This assay is based on detection of mycotoxin-produced DNA alterations arising from recombinational deficiency in rec- cells. Aflatoxin B1 showed a linear dose-response relationship when the inhibition halo was taken as a parameter for the evaluation procedure. Assays carried out with or without hepatic microsomal activation exhibited a similar response.
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PMID:Bacillus subtilis rec Assay for Quantification of Aflatoxins. 3096 51