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Distribution of alkaline phosphatase (ALPase) activity on the plasma membrane of rat hepatocytes in primary monolayer culture was observed using the backscattered electron image (BEI) mode. Apparent reaction product was seen in hepatocytes cultured for 48 or 72 hours in a concentration of 8 X 10(5) cells per ml, and those cultured for 72 or 96 hours in a concentration of 6 or 4 X 10(5) cells per ml. The reaction product was observed as scattered fine dots, as spots, and as bands along the cell edge, with high contrast in flattened polyhedral hepatocytes forming cell trabeculae. The deposition of the reaction product was generally more abundant at the periphery of the cell trabeculae. X-ray microanalysis revealed that the reaction product contains both lead and phosphorus as real ALPase reaction product. In transmission electron microscopy, the reaction product was exclusively localized on the external surface of the plasma membrane. However, the plasma membrane adjoining the culture substratum was devoid of the reaction product. Further, the highest biochemical ALPase activity appeared in earlier culture stages when the density of cultured cells was larger. Thus, during a few days of culture, ALPase activity increases on the plasma membrane predominantly at the periphery of the hepatocyte trabeculae. This increase in the activity might be related to the mechanism of preserving the cell shape.
Anat Rec 1983 Dec
PMID:Observation of cytochemical alkaline phosphatase activity on the plasma membrane of cultured rat hepatocytes by backscattered electron imaging. 636 42

In a five year experiment, 195 lambs and their dam were kept on four plots of wet moorland rich in the plant Narthecium ossifragum. The plots were top-dressed with calcium and, or, phosphorus, or given no treatment. After three years, the plant disappeared from the plots to which phosphorus had been applied, but remained where calcium only had been used. The saponin content of the plant appeared to be uninfluenced by the type of top-dressing or time of year. Photosensitisation (alveld) occurred on all plots during the first three years. During the fourth year the disease occurred only where the plant persisted. In the fifth year the disease did not occur on any plot. Alveld was produced in two lambs by the repeated administration of large quantities of saponin preparations from N ossifragum. Lambs of haemoglobin type AA were significantly more resistant to the disease than lambs with type BB. In nearly half the cases, the bromsulphthalein liver function test indicated a susceptibility to alveld up to seven days before the appearance of clinical signs. Increased bromsulphthalein retention was more common amongst AA lambs than BB lambs, but nearly all the BB lambs developed alveld, and only a few of the AA lambs. The differences were significant.
Vet Rec 1983 Apr 16
PMID:Bog asphodel (Narthecium ossifragum) as a cause of photosensitisation in lambs in Norway. 685 80

During the summer of 1992 renal failure was diagnosed in 232 grazing cattle in 85 herds on the west coast of Norway. The salient clinical signs were depression, anorexia and melaena or fresh blood in the faeces; diarrhoea was also commonly observed. The serum concentrations of creatinine, urea, magnesium and phosphorus, and the activities of glutamate dehydrogenase, aspartate aminotransferase and creatine kinase were above normal and the serum calcium concentration was below normal. Post mortem examinations consistently revealed renal tubular necrosis. In some cases there was liver necrosis and also erosions at the base of the tongue, in the oesophagus and in the jejunum and colon. The toxicity was probably caused by the plant Narthecium ossifragum (bog asphodel).
Vet Rec 1995 Sep 09
PMID:Nephrotoxicity of Narthecium ossifragum in cattle in Norway. 750 63

Forty-two mineral balances were measured in 50 kg pigs fed various diets, including barley, maize or potato, and used to examine the presumption that the current recommended dietary requirements for the major minerals, especially phosphorus, are essential to the well-being of the pig. All the diets contained similar levels of all the mineral elements, except potassium which was higher in the diets containing potato. The concentrations/kg dry matter were 11.0 g calcium, 6.9 g phosphorus, 0.96 g sodium, 1.5 g magnesium and 5.4 g potassium or 14.0 g potassium in the diets containing potato. The apparent digestibility coefficients were calcium 0.39, phosphorus 0.46, sodium 0.72, magnesium 0.26 and potassium 0.70 or 0.80 and the gross efficiencies with which the ingested elements were retained were calcium 0.36, phosphorus 0.25, sodium 0.32, magnesium 0.07 and potassium 0.22 or 0.16. The low efficiencies of utilisation of the digested minerals, especially phosphorus (0.53), suggested that a reduction in dietary phosphorus levels may be justified in terms of reducing the pollution of the environment with phosphorus caused by the application of pig slurry.
Vet Rec 1995 Aug 26
PMID:A preliminary re-assessment of the requirements for major minerals by growing pigs. 750 73

In two sets of experiments eight groups of seven-week-old pigs weighing about 9 kg were fed for three weeks a prestarter that contained 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 10.0 or 15.0 mg/kg of highly purified T-2 toxin. The feed of the two control groups was free from T-2 toxin. Average daily intakes of toxin by the pigs were 0.38, 0.81, 1.24, 1.43, 0.93, 0.81, 0.99 and 2.5 mg, respectively. The weight gains, the feed intakes, the extent of feed refusal, the parameters of energy and protein metabolism and the serum concentrations of calcium, inorganic phosphorus and magnesium were affected to different extents by the different doses of T-2 toxin, but the data indicated that feed consumption was reduced and the activity of aspartate aminotransferase was increased by the smallest amount of T-2 toxin tested.
Vet Rec 1995 May 13
PMID:Effect of various levels of T-2 toxin on the clinical status, performance and metabolism of growing pigs. 764 84

Reference values for some haematological and plasma biochemical constituents were established in Spanish ibex (Capra pyrenaica hispanica) restrained either physically or chemically with tiletamine-zolazepam. The following variables were studied: haematocrit, haemoglobin concentration, total erythrocyte and leucocyte counts, haematological indices, erythrocyte dimensions, differential count of leucocytes, glucose, urea, uric acid, cholesterol, triglycerides, creatinine, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine phosphokinase, lactic dehydrogenase, gamma-glutamyl transpeptidase, total plasma protein, albumin, globulins, albumin-globulin ratio, sodium, potassium, calcium, magnesium, total phosphorus, chloride and osmolality. No haematological data have been published before but the values observed were in the general range of other artiodactyls, with the exception of the number and size of the erythrocytes which were respectively larger and smaller than in most other ruminants. Significant differences were found for a number of the variables between the values recorded in physically restrained animals and the values recorded in anaesthetised animals; they included the number of erythrocytes and related parameters, the plasma proteins and some inorganic ions.
Vet Rec 1993 Jun 05
PMID:Haematology of Spanish ibex (Capra pyrenaica hispanica) restrained by physical or chemical means. 833 2

A study was made of the effects of exogenous adrenocorticotropin (ACTH) on the levels of blood components in 109 dairy replacement calves and the statistical correlations between these effects and the growth rates of the calves from birth to six months. Blood samples were taken from a jugular vein before ACTH was injected and then at two, four, six and eight hours afterwards, and analysed for plasma cortisol concentration, total white cell counts, packed cell volume, haemoglobin, plasma glucose, sodium, potassium, magnesium and inorganic phosphorus, erythrocyte sodium, potassium and magnesium, serum ionised calcium and total protein and total plasma calcium concentration. The injection of 1.1 +/- 0.02 iu/kg of ACTH intramuscularly resulted in a peak plasma cortisol concentration after two hours which had not returned to normal after eight hours. It also resulted in leucocytosis, lymphopenia, neutrophilia, eosinopenia and hypophosphataemia; the mean changes were repeatable (P < 0.05) in 49 of the calves tested two months later. The weight gains to six months of age could be predicted from the degree of the changes in several blood constituents. Significant partial regression coefficients were found for the change in glucose concentration (0 to four hours), absolute neutrophil count (0 to two hours), absolute lymphocyte count (0 to four hours) and loge absolute eosinophil count (0 to two hours). The multiple regression sum of squares was highly significant (P < 0.0001), and the multiple coefficient of determination was 0.305. It was concluded that the changes in these blood components after an injection of ACTH might be used to predict the weight gains of dairy replacement calves.
Vet Rec 1995 Jul 08
PMID:Responses of calves to injections of ACTH and their relationship with growth rate. 852 81

Poor calcification of the teeth and the bones of the skull predisposes pet rabbits to dental disease. This study is a preliminary investigation into the dietary habits of pet rabbits. Owners were questioned about the feeding preferences of their pets. Manufacturers of rabbit foods were asked about the calcium, phosphorus and vitamin D content of their foods and how they had decided upon the formulation of their rations. Samples of rabbit food were analysed for calcium and phosphorus. Rabbits were found to be selective feeders. Rabbit food from pet shops consists of a mixed ration, of which the most commonly rejected ingredients were pellets and whole grain. The food manufacturers reported that calcium, phosphorus and vitamin D supplements are incorporated into the pellets. Food analyses demonstrated that rejection of the pellets and whole grain from the food can reduce a rabbit's calcium intake to below the minimum dietary requirement. The rabbit's unusual calcium metabolism is discussed. Calcium deficiency may cause osteomalacia but dietary excess may cause urolithiasis. Vitamin D deficiency may also exacerbate calcium deficiency. Recommendations are made for preventing calcium deficiency and dental disease in rabbits.
Vet Rec 1996 Dec 07
PMID:Calcium deficiency, diet and dental disease in pet rabbits. 912 6

Six of the 33 calves born in a Swedish dairy herd during a period of four months developed laminitis when they were eight to 12 weeks old. The clinical signs included difficulty in rising, a stiff gait, overgrown claws and haemorrhages in the sole horn. Samples of blood were taken from four of the calves when they had shown signs of laminitis for two to seven weeks; the serum concentrations of calcium, phosphorus and vitamin D3, the activities of aspartate aminotransferase and glutathione peroxidase, and the patterns of serum proteins were within their normal ranges. The feet of the same four calves were examined after slaughter; the third phalanx of each calf was rotated and its distal end osteolytic. Histologically there was separation and degeneration of the squamous cells of the white line, and thromboses and vasculitis in the fine vessels of the corium. Four of the six affected calves were persistently infected with bovine viral diarrhoea virus and one had antibodies against the virus. From six weeks of age the calves had been fed rye wheat, a hybrid seed rich in starch, and this may have contributed to the outbreak of laminitis.
Vet Rec 1997 May 31
PMID:Laminitis in young dairy calves fed a high starch diet and with a history of bovine viral diarrhoea virus infection. 919 98

The relationships between some soil parameters and blood glutathione peroxidase (GSHPx) activity in 15-day-old lambs was studied on 18 sheep farms. Soil samples were analysed for the proportions of sand, silt and clay, pH, and the concentrations of calcium, phosphorus, iron, nitrogen and sulphates. On the farms where the lambs had adequate GSHPx activity (> or = 130 i.u./g Hb) the soils contained significantly more clay, phosphorus and sulphates, and significantly less iron and nitrogen, than on the farms where the lambs did not have adequate GSHPx activity.
Vet Rec 1997 Sep 13
PMID:Relationships between some soil parameters and the blood glutathione peroxidase activity of grazing sheep. 931 40


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