Gene/Protein Disease Symptom Drug Enzyme Compound
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The present study investigated the immunohistochemical localization of heat shock protein 25 (HSP 25) of rat molar teeth during root formation. Most, probably all, cells of the epithelial rest of Malassez (ERM cells) had immunoreaction for laminin, a marker protein for basement membrane. During root formation, HSP 25 immunoreactivity was observed in odontoblasts, cells at the subodontoblastic layer, and those in close proximity to the acellular cementum. HSP 25-immunopositive cells at the subodontoblastic layer were present only at the apical region. Most HSP 25-immunoreactive cells in close proximity to the cementum lacked laminin immunoreactivity. However, at postnatal day 28 a small number of cells showed immunoreaction for both HSP 25 and laminin at the cervical and bifurcational regions. Under the electron microscope, most HSP 25-immunoreactive cells along the surface of the cementum were round and contained rich organelles such as mitochondria and rough endoplasmic reticulum. They lay between fiber bundles of the periodontal ligament. The localization and morphological features of these HSP 25-immunoreactive cells resemble those of cementoblasts. On the other hand, HSP 25-immunoreactive cells at the cervical region were oval and contained few cell organelles. They were closely apposed to each other, and separated from the surrounding tissues with basal lamina. These features were similar to those of mature ERM cells. In contrast, cells with microvillus-like processes and relatively rich mitochondria, which were similar to immature ERM cells, had no immunoreaction for HSP 25. These results suggest that HSP 25 may be involved in shape alterations of ERM cells, cementoblasts, and odontoblasts during differentiation.
Anat Rec 2002 Aug 01
PMID:Immunohistochemical localization of heat shock protein 25 (HSP 25) during root formation of the rat molar. 1212 10

Cells in the epithelial rest of Malassez (ERM cells) express calbindin D28k (CB); however, the hormonal regulation of CB in ERM cells remains to be elucidated. We investigated the immunohistochemical localization of CB and 1,25-dihydroxyvitamin D3 receptor (VDR) during root formation of mouse molar teeth in order to clarify whether the expression of CB in ERM cells is dependent on vitamin D. At the early stage of root formation (postnatal (PN) days 10-14), both CB- and VDR-immunoreactive cells were observed intermittently along the root surface. In the apical portion, almost all CB-immunoreactive cells showed VDR immunoreactivity; however, VDR-immunoreactive cells in the most apical portion were immunonegative for CB. In the middle and cervical portions, the distributions of the two proteins were completely different. At the late stage of root formation (PN28d) and in adult animals, CB immunoreactivity was distributed in cells found along the acellular cementum at the bifurcation region, as well as between the dentin and cellular cementum in the apical portion (although these lacked immunoreactivity for VDR). The present results indicate that CB expression in newly disrupted cells from Hertwig's epithelial root sheath occurs in a vitamin-D dependent manner, whereas the expression of CB in mature ERM cells may be independent of vitamin D.
Anat Rec A Discov Mol Cell Evol Biol 2003 Aug
PMID:Immunolocalization of calbindin D28k and vitamin D receptor during root formation of murine molar teeth. 1284 6