Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q9UIJ5 (Rec)
 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A number of transition metal complexes, including the cis and trans isomers of dichlorodiammineplatinum (II), six complexes of rhodium (I), two of iridium (I), and one of ruthenium (II) have been tested for their ability to induce lambda prophage, to produce filamentous growth of Escherichia coli, and to be selectively toxic for strains with defects in the deoxyribonucleic acid repair system. Dichlorotetrakis(dimethylsulfoxide)ruthenium II [RuCl(2) (DMSO)(4)] was strictly similar to cis-dichlorodiammineplatinum II [cis PtCl(2) (NH(3))(2)] in the test for lambda induction, filamentous growth production, and selective toxicity for a recA(-) strain. [Rh COD 1,10-phenanthroline](+) Cl(-), though more toxic for recA(-) than for rec(+)E. coli, was scarcely effective in the test for filamentous growth and did not induce prophage. None of the other tested compounds showed any similarity with cis-PtCl(2)(NH(3))(2). Due to the interesting results obtained with cis-PtCl(2)(NH(3))(2) as an antitumor agent, it seems reasonable to propose RuCl(2)(DMSO)(4) as a potential antitumor substance.
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PMID:Effects of cis-Dichlorudiammineplatinum (II) and related transition metal complexes on Escherichia Coli. 109 72

Three-dimensional alteration of fibrillar matrix in the rat mandibular condylar cartilage was investigated with a high-resolution scanning electron microscope (SEM) and it was determined whether alterations correlate with developing occlusion and advancing age. Two important SEM techniques of DMSO freeze-cracking and treatment with trypsin and hyaluronidase were employed to remove interfibrillar proteoglycans and disclose fibril arrangement. Our SEM investigation demonstrated that collagen fibrils in the fibrous zone covering hyaline-cartilaginous area in the condyle are thicker (50 to 80 nm in diameter) than the fibrils (30 to 50 nm in diameter) that predominantly constituted an interterritorial fibrillar matrix (IFM) in the area. While the thick fibrils had a distinct striation of about 55 nm periodicity, the thin fibrils had no distinguishable striation. The thick fibrils having a periodic striation of about 60 nm was found along with the thin fibrils, also in the IFM in the aged rats and in the deep IFM, but were considerably less than the thin fibrils. The fibrils in the fibrous zone and IFM were disorderly arranged at 19-day-insemination age. In 1-week-old rats whose incisors erupted, the fibrils constituting the fibrous zone altered from disordered to ordered arrangement. The IFM in these rats took the form of a network. Incorporation of small fibrillar bundles into the fibrillar network was seen in 2-week-old rats whose upper and lower first molars erupted. In 8-week-old rats whose molars had erupted completely, the IFM completely occupied by regularly oriented fibrils appeared additionally.(ABSTRACT TRUNCATED AT 250 WORDS)
Anat Rec 1992 Dec
PMID:Ultrastructural alteration of cartilaginous fibril arrangement in the rat mandibular condyle as revealed by high-resolution scanning electron microscopy. 145 52

The three-dimensional structure of the transverse-axial tubular system, sarcoplasmic reticulum (SR), and intercalated disc of the rat left ventricle was examined by high-resolution scanning electron microscopy after removal of the cytoplasmic matrices by the osmium-DMSO-osmium procedure. In the intermyofibrillar space, the transverse tubules (T-tubules) are accompanied by longitudinally oriented axial tubules and together form a transverse-axial system. The junctional SR is usually small but occasionally medium or large in size and couples with the T- or with the axial tubules. On the surface of the junctional SR facing the T- or the axial tubule, tiny junctional processes are seen. One or two sarcotubules, the so-called Z-tubules, frequently run parallel to the T-tubule. The sarcotubules derived from the junctional SR or from the Z-tubule run longitudinally or obliquely and form polygonal meshes around the myofibrils. On the surface of the SR at the H-band level, small fenestrations of 12-40 nm in diameter, and tiny hollows 8-20 nm in diameter are seen. Bulbous swellings of the SR, the corbular SR, are preferentially seen near the Z-band. The large and flat SR, known as the cisternal SR, intercalates among the SR meshes. In the subsarcolemmal space, the sarcotubules form a multilayered network (peripheral SR). The cisternal SR is frequently intercalated in these meshes and closely associated with the inner surface of the sarcolemma. The intercalated disc appears as a prominently undulated membrane demarcating the border between two adjacent heart muscle cells, and occasionally small projections 60-90 nm in diameter and 200-600 nm in length display on its surface.
Anat Rec 1990 Nov
PMID:High-resolution scanning electron microscopic studies on the three-dimensional structure of the transverse-axial tubular system, sarcoplasmic reticulum and intercalated disc of the rat myocardium. 226 Jul 83

Morphologic changes in the development of the mitochondrial helical sheath in the mouse spermatid tail were examined with the scanning electron microscope (SEM) using the osmium-DMSO-osmium method and classified into several stages. During late spermiogenesis, spherical mitochondria gathered around the forming spermatid tail. The shape of these mitochondria gradually changed from spheroid to long and rod-like. Mitochondria first were arranged in four longitudinal rows (stage 1) that twisted dextrally, and the mitochondria began to stagger (stage 2). They became elongated and arranged into a staggered pattern; they then attached to each other in an end-to-end fashion to form a sinistral double helix around the core of the axoneme (stage 3). These end-to-end contacts were observed in every second gyre on the four lines surrounding the core of the axoneme at stage 3. Mitochondria further elongated and end-on touching appeared with every third gyre on the five longitudinal lines that surround the core of the axoneme (stage 4). The direction of the helix, always sinistral, was clearly discernible only in the later stages. Disposition of the mitochondria in the spermatid tail was regular throughout development, which indicates that these mitochondria elongate simultaneously and also at the same rate. On any given cracked surface of the seminiferous tubule, spermatid tails with the same stage of mitochondria predominantly were observed. This ultrastructural finding appears compatible with the histologic synchronism, (termed the "wave") in differentiating germ cells.
Anat Rec 1988 Sep
PMID:Development of mitochondrial helical sheath in the middle piece of the mouse spermatid tail: regular dispositions and synchronized changes. 318 85

Hexachloroacetone, CCl3--CO--CCl3, reverts the Ames strains TA98 and TA100 but not the non-plasmid strains TA1537, TA1535 and TA1538. In the absence of solvent, the number of revertant colonies is 5 times the spontaneous reversion rate for TA100 and 10 times the spontaneous reversion rate for TA98 with 26 mg hexachloroacetone per plate. This effect is seen in the absence of rat liver microsomes. In dimethylsulfoxide (DMSO) solution a more complicated pattern is seen. In DMSO solution cooled between 18 and 20 degrees C, The maximum nuber of revertants is similar to that found in the absence of DMSO, but only 1.75 mg hexachloroacetone per plate is needed. When DMSO solution of hexachloroacetone is warmed above 20 degrees C, a yellow color develops and the solution becomes more toxic to the test bacteria. The maximum number of revertants is then produced at about 0.5 mg hexachloroacetone per plate. Hexachloroacetone is found to be active, without microsomal activation, in the E. coli WP-2 and E. coli rec-BC test systems. Hexachloroacetone readily reacts with water in DMSO solutions to form the non-mutagenic hexachloroacetone hydrate.
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PMID:The mutagenic properties of hexachloroacetone in short-term bacterial mutagen assay systems. 702 27

The three-dimensional organization of the membrane system of the rat parietal cells in the resting state and during early stimulation with tetragastrin (gastrin) was determined by ultra-high-resolution scanning electron microscopy. Specimens were prepared by cytoplasmic matrix removal using the aldehyde-osmium-DMSO-osmium procedure. The intracellular canaliculus was lined with numerous microvilli. Viewed from the cytoplasmic side, the intracellular canaliculi appeared as an arborized system of cactus-like structures with numerous round holes about 100 nm in diameter corresponding to the basal openings of the microvilli. The intracellular canaliculi were more developed after gastrin stimulation than in the resting state. In resting cells, most of the tubulovesicles were isolated, 100-200 nm in diameter, spherical or tubular in shape, and had a smooth surface. After gastrin stimulation, these structures were interconnected by slender tubules of about 30 nm in diameter forming together tubulovesicular network. Occasionally, swollen and shrunken profiles were observed. The tubulovesicular network was connected with the intracellular canaliculus only at a few sites by the slender connecting tubules. Fusion of the tubulovesicular network with the intracellular canaliculus is observed at such sites. In the fasted rat, the microvilli were slender and their interior was packed with some kind of ill-defined material, probably microfilaments. However, after gastrin stimulation, the microvilli were swollen and their interior was almost empty. These morphological changes seem to indicate the accumulation of fluid in the microvilli after gastrin stimulation, with subsequent swelling.
Anat Rec 1993 Oct
PMID:Ultra-high-resolution scanning electron microscopic studies on the membrane system of the parietal cells of the rat in the resting state and shortly after stimulation. 823 72

Cell shape and density are critical to the evaluation of neutrophil function and/or activation. Dimethyl sulfoxide-cryofixation-freeze-substitution processing (DCF) instantly preserves cell processes and ultrastructural elements with fewer artifacts than routine chemical fixation with glutaraldehyde and postfixation osmium tetroxide (GO). This study morphometrically examined density-separated neutrophils to assess differences in DCF and GO processing procedures and studied the effect of dimethyl sulfoxide followed by GO fixation (DGO) on morphology. Fifteen consecutive neutrophils were analyzed using computerized planimetry for differences in DCF v. GO treatments (n = 4) and DGO v. GO treatments (n = 4). Cryofixed and DGO-fixed cells were significantly rounder than GO cells which had a more irregular surface with membrane projections. The cell volume of GO cells was 27-30% smaller than in DCF or DGO processing, while the surface area was similar. The increased volume in DCF and DGO cells did not appear to be due to abnormal cell swelling, since membranes, nuclear envelope, and mitochondrial cristae were more intact than in GO cells. Preservation of mitochondria as well as endocytic caveolae with a subplasmalemmal coating was best in DCF samples, moderate in DGO, and poorest in GO. Morphometric data showed that the nuclear compartment was 22% smaller, while the cytoplasm (and its associated compartments) was 29% smaller in GO compared to DCF-processed neutrophils. This was consistent with the more dense cytoplasm in GO cells. Pretreatment of neutrophils with dimethyl sulfoxide (DMSO) resulted in volume preservation and improved the morphology of GO fixation. In summary, DCF appears to be an excellent method for preserving neutrophil membranes and cytoplasmic organelles (particularly mitochondria), and prevents a number of artifacts caused by routine GO fixation. Morphology can also be improved by using DMSO in conjunction with GO.
Anat Rec 1998 10
PMID:Morphology of human neutrophils: a comparison of cryofixation, routine gluteraldehyde fixation, and the effects of dimethyl sulfoxide. 977 79

In this study, the three-dimensional organization of the Golgi apparatus in mouse spermatids was elucidated by preparing testicular tissue with the osmium-DMSO-osmium method and examining it by stereo-scanning electron microscopy. The cis-most saccule was found to be a regular network of anastomotic membranous tubules covered by a single cisterna of ER. The trans-Golgi network was seen to be composed of irregular saccules perforated by pores at the edge. It appears that the anastomosing trans-Golgi network breaks down into strings of connected vesicles which arise from the edge of the saccules during the cap phase of spermiogenesis. Many apparently individual vesicles seen in thin sections through the trans-Golgi network are actually joined in continuous strings. This was the first time that these structures could be visualized directly without three-dimensional image reconstruction. By correlating the morphology of the Golgi apparatus with the stage of acrosome formation, the Golgi cisternae were found to change dynamically in a cis-trans direction from fenestrated saccules to continuous strings of vesicles, which finally dissipated as transport vesicles at the trans aspect. This suggests that the hypothetical model of cisternal maturation, which dictates that cargo moves through the Golgi apparatus without leaving the cisternal lumen and the secretion occurs by progressive maturation of the Golgi cisternae as they move in the cis-trans direction, may be applicable to acrosome formation.
Anat Rec 1999 10 01
PMID:Three-dimensional structure of the Golgi apparatus in mouse spermatids: a scanning electron microscopic study. 1048 16

Using a recently developed fixation technique for parietal cells (Sugai et al., Acta Anat Nippon 1995:70:S79, 1999:74:S101), we have reinvestigated the organization of the cytoplasmic membrane system in the resting stomach by ultra-high-resolution scanning electron microscopy (SEM). Rat gastric mucosae were microwave-fixed in cacodylate buffer [334 milliosmoles/kg H(2)O (mOsm)], to which 1.0% glutaraldehyde and 0.5% formaldehyde were added. Specimens examined by transmission electron microscopy (TEM) of thin sections revealed cytoplasm packed with tubular membranes similar to images detected by rapid-freeze/freeze-substitution fixation which is generally considered to cause minimal structural alterations. To render the cytoplasmic membranes visible by SEM, fixed mucosae were frozen, fractured, and the exposed cytoplasm of parietal cells was macerated by the aldehyde-osmium-DMSO-osmium procedure. With much of the cell matrix and filaments removed, SEM revealed numerous 30-60 nm tubules which formed a meshwork and also small cisternae. The cytoplasmic surface of the tubules was smooth while some cisternal areas had attached polyribosomes. Vesicles or isolated tubules were not found in appropriately macerated parietal cells. The cytoplasmic surface of the intracellular canaliculus was smooth except for round openings representing the bases of macerated microvilli. In favorable sites connections of the tubular membranes to the canaliculi were clearly visible. Stereo pair views were particularly useful to demonstrate these continuities. Connections between these two membrane compartments suggest the probability of rapid membrane transposition.
Anat Rec 2000 01 01
PMID:Scanning EM of resting gastric parietal cells reveals a network of cytoplasmic tubules and cisternae connected to the intracellular canaliculus. 1060 44

Retinoic acid has been associated with a variety of cardiac defects. A percentage of these defects are related to changes in the endocardial cushions. Studies in mice and older chick embryos have shown a decrease in mesenchymal cell formation attributable to retinoic acid and have suggested that retinoic acid was affecting the extracellular matrix. In this study we have tested the effect of retinoic acid on cardiac mesenchyme formation in vitro and then tested retinoic acid treated myocyte cultures for changes in the expression of hLAMP-1, fibronectin and transferrin members of the particulate matrix that is required for mesenchyme formation. Initial experiments tested the effect of retinoic acid on mesenchymal cell formation first in atrioventricular canal and outflow tract explant cultures and then in AV endothelial monolayer cultures using myocyte conditioned media or the particulate matrix fraction from retinoic acid treated myocyte cultures. In all cases, mesenchymal cell formation was suppressed while no suppression was observed when MyoCM was included with retinoic acid. Protein analysis showed that retinoic acid had a stimulatory effect on protein synthesis. ELISA assays revealed that retinoic acid treated myocyte cultures contained significantly more hLAMP-1 and fibronectin than either normal or DMSO controls. However, transferrin was not affected by retinoic acid treatment in these experiments. Our results suggest that retinoic acid affects the expression of the particulate matrix and that these changes may be responsible for the observed decrease in mesenchymal cell formation.
Anat Rec 2000 02 01
PMID:Retinoic acid inhibition of cardiac mesenchyme formation in vitro correlates with changes in the secretion of particulate matrix from the myocardium. 1064 66


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