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Dioxidine pharmacokinetics was studied in 5 patients operated for cancer of the large intestine and treated prophylactically with the drug during the postoperative period. Dioxidine was administered intravenously for 10 minutes twice a day in an amount of 300 mg in 5 per cent solution of glucose. The drug concentrations in serum and urine were determined with a microbiological procedure. Escherichia coli AB 2472 rec A16, a strain deficient with respect to reparation was used as a test microbe. The plates with the dilutions were incubated under anaerobic conditions. The time course of the drug concentrations in serum was shown to be satisfactorily described by the following equation: C(t) = 3.125 . 1-2.57.t + 2.76 . 1-0.64.t. Within the first 1.5-2 hours after the administration the dioxidine concentrations in serum and urine amounted to 2.5-4 and 35-50 micrograms/ml respectively.
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PMID:[Pharmacokinetics of dioxidine in oncological patients in the postoperative period]. 269 30

Labeling of hepatic glycogen derived from 3H-galactose and 3H-glucose was compared shortly after intravenous injection in control-fed rats. The rats were allowed to accumulate 5-8% glycogen prior to receiving label. Fifteen minutes to 2 hours after labeling, liver was excised and processed for routine light (LM) and electron microscopic (EM) radioautography (RAG) or biochemical analysis. After injection of 3H-galactose, LM-RAGs revealed that the percentage of heavily labeled hepatocytes increased from 37% after 15 minutes to 68% after 1 hour but showed no further increase after 2 hours. alpha-Amylase treatment removed most glycogen and incorporated label; thus few silver grains were observed, indicating little incorporation of label except into glycogen. EM-RAGs demonstrated that most label occurred where glycogen was located. Biochemical analysis showed initially a high blood level of label that rapidly plateaued at a reduced level by 5 minutes. Concomitantly, glycogen labeling determined by liquid scintillation counting reflected the increases observed in the RAGs. After injection of 3H-glucose, LM-RAGs revealed that only 12% of the hepatocytes were heavily labeled at 1 hour and 20% at 2 hours. In tissue treated with alpha-amylase, glycogen was depleted and label was close to background level at each interval observed. EM-RAGs showed most grains associated with glycogen deposits. Biochemically, blood levels of label persisted at a high level for 30 minutes and tissue levels increased slowly over the 2-hour period. This study shows that incorporation from 3H-galactose was more rapid than incorporation of 3H-glucose; however, label derived from both carbohydrates appeared to be incorporated mainly into glycogen.
Anat Rec 1989 May
PMID:Comparison of 3H-galactose and 3H-glucose as precursors of hepatic glycogen in control-fed rats. 278 54

Cytochemical and biochemical glucose 6-phosphatase (G6Pase) activity was examined in brown adipose tissues of normal, cold-exposed, or starved mice. In addition, G6Pase activity in white adipose tissue and hexokinase activity in brown and white adipose tissues were biochemically measured. In normal animals, the reaction product for G6Pase activity was localized in the endoplasmic reticulum and nuclear envelope of brown adipose cells. The amount of the reaction product increased in cold-exposed or starved animals. Biochemical G6Pase activity (259.7 +/- 48.5 ng Pi/min/mg protein) in brown adipose tissues of normal animals was higher when the value was compared with values of other organs. Biochemical G6Pase and hexokinase activities increased rapidly in brown adipose tissues of cold-exposed animals, and a close relation was found between activities of the two enzymes. In brown adipose tissues of animals starved for 3 days, biochemical G6Pase activity increased, but hexokinase activity did not change. In white adipose tissues of normal, cold-exposed, or starved animals, G6Pase activity was very low, although the enzyme activity increased slightly in animals starved for 3 days. The results show that the high G6Pase activity in brown adipose cells probably relates to thermogenesis in cold-exposed animals and may be concerned with glucose release into the blood in starved animals.
Anat Rec 1987 Sep
PMID:Significance of increase in glucose 6-phosphatase activity in brown adipose cells of cold-exposed and starved mice. 282 61

Glycogen, glycogen phosphorylase, and glucose 6-phosphatase (G6Pase) activities were examined cytochemically in chondrocytes of femoral epiphyseal cartilages and cartilaginous ribs of 3- and 7-day-old rats. G6Pase activity was also examined biochemically. Glycogen was abundant in chondrocytes of the reserve zone, while it became scarce in the cells of the proliferative zone. From the upper part (adjoining the proliferative zone) to the lower part of the hypertrophic zone, glycogen accumulated in chondrocytes and decreased in the cells of the degenerative zone. Inversely, glycogen phosphorylase a and G6Pase activities were relatively high in chondrocytes of the proliferative zone and upper hypertrophic zone and were low in the cells of the reserve zone, lower hypertrophic zone, and degenerative zone. The reaction product for G6Pase was present in the endoplasmic reticulum and nuclear envelope of all types of chondrocytes composing the cartilages, although the amounts of reaction product varied with the cell types in parallel with the histochemical results. Biochemical G6Pase activity was higher in epiphyseal cartilages than in cartilaginous ribs. The possible mechanism and significance of the accumulation and decrease of glycogen in chondrocytes of the epiphyseal cartilage were discussed.
Anat Rec 1987 Dec
PMID:Glucose 6-phosphatase and glycogen phosphorylase activities in chondrocytes in epiphyseal cartilage of growing rats. 283 83

Acute renal failure was diagnosed by clinical, necropsy and histological criteria in 39 flocks (20 low ground, 13 hill and six marginal upland) in areas served by six veterinary investigation centres. Forty-eight lambs of 12 different breeds or crosses were investigated. The mean age of affected lambs was 38 days (range seven to 84 days); 21 lambs (44 per cent) were aged seven to 28 days, while only eight (17 per cent) were older than two months. Mortality in clinically affected lambs was almost 100 per cent, with no response to various treatments. Histological examination showed that 40 lambs (83 per cent) had nephrosis, while the rest had toxic tubular necrosis, interstitial nephritis or tubular damage associated with oxalate crystal deposits. Only about half of the lambs had any evidence of enteric infections or enteropathy. Acutely ill lambs had azotaemia, haemoconcentration and proteinuria; some lambs had glycosuria or haematuria. Samples of plasma from 22 lambs with nephrosis were compared with similar samples from 82 incontact but asymptomatic lambs. The clinically affected group had significantly elevated plasma urea, creatinine, total protein, globulin, phosphorus and chloride concentrations and significantly reduced plasma calcium concentrations compared with healthy lambs. Affected lambs had a significant reduction also in the calcium:phosphorus ratio. No significant differences between groups was found in plasma concentrations of albumin, glucose, lactate, glycerol, creatine kinase, alkaline phosphatase, sodium, potassium or magnesium.
Vet Rec 1989 Jan 07
PMID:Acute nephropathy in young lambs. 291 11

Fetal rat hepatocytes (22-day-old, full-term) in vivo were homogeneous in ultrastructure and glucose 6-phosphatase (G6Pase) distribution throughout the liver acinus. These cells were isolated and cultured for 17 days in Williams medium E containing 10% fetal calf serum, dexamethasone, insulin, and glucagon. Heterogeneity among hepatocytes appeared progressively during culture. There were variations in numbers of binucleated cells, cytoplasmic ultrastructure (e.g., in the distribution of glycogen and the quantity of ultrastructure (e.g., in the distribution of glycogen and the quantity of mitochondria), and intensity of histochemical reactivity for G6Pase. The cells could be classified generally into two types: type I cells had some characteristics of periportal hepatocytes and type II cells those of centrolobular hepatocytes in in vivo adult liver. The results show that a functional and structural heterogeneity can be formed among fetal hepatocytes in monolayer culture without differences in supply of oxygen, nutrients, and hormones. A hidden heterogeneity might already exist among hepatocytes in full-term fetuses even though it cannot be detected ultrastructurally or cytochemically.
Anat Rec 1985 Oct
PMID:A functional and structural heterogeneity is formed among fetal rat hepatocytes during culture. 300 Feb 23

Cytochemical and biochemical glucose 6-phosphatase (G6Pase) activity and fiber type composition were studied in soleus (SOL) and gastrocnemius (GC) muscles of mice. The SOL is a red muscle which contains numerous type I fibers (60%) and relatively few type II fibers (40%). The GC is a white muscle which contains numerous type II fibers (90-100%) and very few type I fibers (0-10%). In the SOL and GC, cytochemical G6Pase activity was localized in the sarcoplasmic reticulum, lateral elements of triads, myonuclear envelope, and in the endoplasmic reticulum and nuclear envelope of endothelial cells. Differential centrifugation showed that G6Pase activity was recovered in the 105,000g pellet (microsomal fraction). Histochemical enzyme activity in type II fibers was slightly higher than that in type I fibers. Biochemical G6Pase activity in the GC was significantly higher than that in the SOL. The possible functional significance of G6Pase in skeletal muscles was discussed.
Anat Rec 1986 Jan
PMID:Cytochemical and biochemical glucose 6-phosphatase activity in skeletal muscle cells of mice. 300 47

Glucose 6-phosphatase activity was studied in the secretory epithelial cell and other cell types composing alveoli of the mammary gland (cytochemical study) and in the whole mammary gland (biochemical study) of pregnant and lactating mice. The reaction product for the enzyme activity was seen in the endoplasmic reticulum and nuclear envelope in secretory epithelial cells from all animals examined (days 7 and 14 of pregnancy, and days 0, 3, 10, and 20 of lactation. The amounts of the reaction product appeared scarce at day 7 of pregnancy, moderate at day 14 of pregnancy and day 0 of lactation, and abundant at days 3 and 10 of lactation. The reaction product, however, became generally scarce at day 20 of lactation. Biochemical activity was relatively low at days 7 and 14 of pregnancy and days 0 and 20 of lactation, while it was high at days 3 and 10 of lactation. The increased activity is probably related to functions of secretory epithelial cells in the lactating gland.
Anat Rec 1986 Apr
PMID:Glucose 6-phosphatase activity in pregnant and lactating mammary glands of the mouse. 301 Jul 79

The effects of starvation on glucose 6-phosphatase (G6Pase; EC 3.1.3.9., D-glucose 6-phosphate phosphohydrolase) and glycogen phosphorylase (EC 2.4.1.1.) activities, and on glycogen content, were studied in skeletal muscles (m. rectus femoris) of mice. In the muscle cells from fed animals, the cytochemical reaction product for G6Pase activity was observed in moderate amounts in the terminal cisternae of sarcoplasmic reticulum and in small amounts in the nuclear envelope, and was rare or absent in the intermyofibrillar sarcoplasmic reticulum. After 4 days of starvation, however, the reaction product became abundant in all of the terminal cisternae, intermyofibrillar sarcoplasmic reticulum, and nuclear envelope. Biochemical G6Pase and glycogen phosphorylase a (active form) activities were higher in the muscles of starved mice than in those of fed animals. The glycogen content decreased markedly in the muscles of starved mice. The results suggest that the role of the increased G6Pase in skeletal muscle cells of starved mice is to release glucose into the blood by hydrolyzing glucose 6-phosphate produced through the increased phosphorylase activity.
Anat Rec 1986 Oct
PMID:Significance of the increase in glucose 6-phosphatase activity in skeletal muscle cells of the mouse by starvation. 302 18

Blood samples were collected from unstressed cattle and from cattle undergoing handling stress, transport stress and slaughter. The blood was analysed for ACTH, cortisol, thyroxine stimulating hormone, tri-iodothyronine (T3) and catecholamine concentrations, and for haematocrit, total plasma protein, plasma lipid, lactate and glucose concentrations. Compared to control values handling significantly increased T3, cortisol, lipid and lactate concentrations. Compared to handling, transport stress was associated with increased catecholamines and lactate concentrations, a decreased cortisol concentration and similar concentrations of T3, lipid and glucose. Compared to transport, slaughter resulted in high catecholamines, lactate and glucose, and low T3, cortisol and lipid levels. It is concluded that the response to stress has two phases, a hypothalamic-adrenal cortex phase which is associated with perceived environmental stress such as noise, and a sympathetic-adrenal-medulla phase which is associated with neurogenic stress such as transport or specifically the massive sympathetic discharge caused by stunning. Combinations of stresses produce a mixed response.
Vet Rec 1988 Aug 20
PMID:Stress in cattle assessed after handling, after transport and after slaughter. 317 73


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