Gene/Protein Disease Symptom Drug Enzyme Compound
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A method is described that produces sections (15 microns) of large specimens (with dimensions up to 140 x 100 x 200 mm). They are suitable for comparison to CT images of that specimen and can be used as a basis for three-dimensional reconstructions. Frozen unfixed parts of the human body are embedded in polyurethane and scanned with CT. Undecalcified slices, 6 mm thick, of these specimens are cut with a bandsaw. These slices are successfully embedded in polyester resin by means of fixation, dehydration, and subsequent impregnation with this resin. Sections of 15 microns are obtained by cutting the trimmed and sandpapered polyester blocks with an LKB multirange microtome. They are collected on adhesive tape and stained according to Weigert-Azan, sealed between adhesive acetate sheets and photographed on colour slide film. As an example photographs of sections of the human elbow and lumbar spine region are presented.
Anat Rec 1992 Aug
PMID:Plastic embedding procedure for 15 microns sections of large undecalcified tissue blocks. 162 24

Although the periodontal ligament (PL) contains an abundance of glycosaminoglycans (GAGs), there are only a few histochemical studies describing GAGs in the developing PL. In the present study, the relationship between the formation of principal fibers and the molecular species of GAGs in the developing PL was examined by light microscopic histochemistry. Jcl:ICR mice were killed on day 0 to day 28 after birth. Paraffin-embedded tissue sections were routinely made and stained with hematoxylin-eosin (H&E), Azan, or the sensitized high iron diamine (S-HID) procedure combined with enzyme digestions. Before tooth eruption, thin threads of collagen fibers in the PL assembled and constructed principal fibers, which projected from both the side of the alveolar bone and the root of the tooth. After tooth eruption, the principal fibers from both sides were tightly entangled. In the developing PL, the molecular species of GAGs was mainly dermatan sulfate. Moreover, the relative amount of dermatan sulfate increased together with the maturation of the principal fibers, while the principal fibers adjacent to the alveolar bone and cementum contained chondroitin sulfate. These results suggest that dermatan sulfate contributes to collagen fiber assembly in the PL and that chondroitin sulfate relates to PL adhesion to the alveolar bone and to the cementum of the root.
Anat Rec 1999 04 01
PMID:Histochemical studies of glycosaminoglycans in developing periodontal ligaments of ICR mice. 1020 54