Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:Q9UIJ5 (Rec)
 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effects of implantation with different anabolic agents on growth rate, behaviour, carcase parameters and testicular size of bulls were evaluated in an experiment with 361 Friesian bulls about three months old on three farms. Animals were allocated to one of the following treatments: (a) Control; (b) repeated implantation with 36 mg zeranol at intervals of 95 to 124 days; (c) repeated implantation with 20 mg oestradiol benzoate plus 140 mg trenbolone acetate as in (b); (d) repeated implantation with 20 mg oestradiol benzoate plus 200 mg progesterone as in (b); (e) implanted once with 45 mg oestradiol-17 beta. Daily liveweight gain, carcase weight, testicular size and behaviour of the animals were the parameters measured. Repeated implantation of young bulls with hormones, beginning at eight to 10 weeks old, increases liveweight gain and carcase weight from 0 to 15 per cent, reduces aggressive behaviour and testicular size and, in some instances, improves conformation and increases fat cover of the carcase.
Vet Rec 1983 Dec 03
PMID:Effect of repeated implantation with anabolic agents on growth rate, carcase weight, testicular size and behaviour of bulls. 668 30

The total protein of colostral whey from dairy cows as determined by a refractometer were compared with the immune globulin concentration obtained by cellulose acetate membrane electrophoresis and the immunoglobulin (IgA + IgG + IgM) contents determined by radial immunodiffusion. The coefficient of correlation between the results obtained by refractometry and electrophoresis was 0.98 (P < 0.001). The correlation between refractometry and radial immunodiffusion was 0.89 (P < 0.001), while that of electrophoresis and radial immunodiffusion was 0.87 (P < 0.001). Refractometry can be used as a simple, fast and inexpensive indirect method of assessing immunoglobulins in colostral whey.
Vet Rec 1980 Jul 12
PMID:Estimation of bovine colostral immunoglobulins by refractometry. 677 82

The relationship of nascent albumin and hepatocyte organelles was studied with the immunoperoxidase reaction in rats given various drugs to alter cellular albumin content. colchicine was used to increase intracellular albumin. Cycloheximide inhibited synthesis but allowed nascent albumin to remain with its ribosome of origin. Puromycin also inhibited synthesis but released albumin from its ribosome. There was no difference in the appearance of attached ribosomes in hepatocytes from saline-injected rats and those given colchicine or cycloheximide. In these cases, membranes of the endoplasmic reticulum were consistently decorated with ribosomes positive for the presence of albumin antigenicity on their cytosolic surface. The cisternal and cytosolic compartments were negative. The situation after puromycin was different. Here the membranes appeared to be denuded of ribosomes and reaction product, indicative of albumin, was present only on the lumenal surface. To determine whether puromycin had caused the release of ribosomes, sections from puromycin-treated cells were stained nonspecifically with uranyl acetate. This showed that the normal amount of ribosomes was still bound but that they could not be seen when a probe specific only for albumin was used. It appears that nascent albumin can associate with its ribosome within the cytosol. Also, apparently after albumin passes through the membrane of the rough endoplasmic reticulum, it remains attached to its lumenal surface. A model incorporating cytosolic folding of albumin followed by its entropic membrane transit is presented.
Anat Rec 1981 Oct
PMID:Ultrastructural immunocytochemistry of nascent albumin topology: proposed cytosolic folding and membrane transit of the protein. 703 62

The three-dimensional arrangement of mitochondria and endoplasmic reticulum in the red muscle fiber was studied both in thick sections of the rat diaphragm fixed in glutaraldehyde and impregnated with uranyl acetate followed by lead and copper citrate, and in thin sections of glutaraldehyde fixed tissue treated with ferrocyanide-reduced osmium. The mitochondria were located either at the periphery of the fiber, where they were spherical, or between the myofibrils, where they formed longitudinal columns of rectangular, slightly flattened elements. From both types of mitochondria, thin, elongated branches arose at right angles that formed transversely oriented mitochondrial pairs at the I band level. At the periphery of the fiber, the endoplasmic reticulum took the appearance of a subsarcolemmal network of tubular cisternae oriented parallel to the cell surface. In the juxtanuclear region, it was made up of spherical masses composed of tightly knitted tubules that were interconnected by more loosely anastomosed tubules. In between the myofibrils, it was composed of longitudinally oriented repetitive units whose structure varied according to their position in from of the A or I bands of the myofibrils. In front of the A band, the endoplasmic reticulum appeared as a single sheet of anastomotic tubules compressed between the adjacent myofibrils, whereas at the I band level, its tubular elements passed in front and behind the transverse expansions of the mitochondria to form an intricate ultilayered network in from of the Z line.
Anat Rec 1980 May
PMID:Three-dimensional electron microscopy of mitochondria and endoplasmic reticulum in the red muscle fiber of the rat diaphragm. 742 5

Effects of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and protein kinase C (PKC) inhibitors on cell-cell communication were studied in a normal rat liver cell line, clone 9. Communication was observed and quantitated with microspectofluorometric and image analysis techniques following scrape-loading of the cells with lucifer yellow. Lucifer yellow migrated as far as ten cells away from the scraped edge in control populations. Two minute TPA (25-50 micrograms/ml) treatment inhibited dye movement such that the dye remained mainly in the cells at the cut edge. The TPA-induced inhibition of cell-cell communication could be partially blocked by 15 min treatment of the cell populations with the PKC inhibitors trifluoperazine (30 micrograms/ml), staurosporine (2 x 10(-8) or 2 x 10(-6) M), sangivamycin (15 or 200 microM), or a PKC inhibitor peptide (20 micrograms/ml) scraped in at the same time as lucifer yellow. Normal communication was observed in cultures treated only with PKC inhibitors. Lower concentrations of TPA (50 ng/ml-1 micrograms/ml) used for 2 min did not inhibit dye communication. Our results demonstrate the phorbol ester-induced interruption of cell-cell communication. The inhibition of PKC by inhibitors eliminates the effect of TPA on communication. Our data are consistent with a role of PKC in the control of junctional communication.
Anat Rec 1993 Jan
PMID:Regulation of the 12-O-tetradecanoyl-phorbol-13-acetate-induced inhibition of intercellular communication. 841 17

Since conventional chemical fixation may extract tissue components and thus alter structural organization, cryofixation was used to reexamine the ultrastructure of three thick basement membranes: lens capsule, Reichert's membrane, and Engelbreth-Holm-Swarm (EHS) tumor matrix, and two thin basement membranes, those of epididymis and semi-niferous tubules. Cryofixation was achieved by slam freezing followed by either freeze substitution in dry acetone containing 1% osmium tetroxide and 0.05% uranyl acetate or freeze drying in a molecular distillation dryer. The results by both procedures demonstrate that thick basement membranes and the lamina densa of thin basement membranes are composed of a network of anastomosing strands referred to as cords. The cords vary in density and distinctiveness, but their thickness averages 3 to 5 nm in every tissue examined. The spaces separating the cords vary within wide limits, but their mean diameter is approximately 15 nm in every case. Two other common features are 1) the presence within the network of a few 1.5-3.0-nm-thick filaments and 2) 4.5-nm-wide sets of parallel lines referred to as double tracks. When these results are compared with those previously described after conventional fixation, no significant difference is observed in either the cord network or the associated filaments and "double tracks." However, in the thin basement membranes processed by cryofixation, the lamina densa is in direct contact with epithelial cells, whereas, after conventional fixation, the lamina densa is separated from the epithelial cells by a pale layer referred to as lamina lucida or lamina rara. Immunogold labeling of three basement membranes after cryofixation and freeze substitution in acetone containing 0.3% glutaraldehyde yields strong reactions for laminin, type IV collagen, and heparan sulfate proteoglycan. Comparison with previous results indicates that conventional formaldehyde fixation adequately preserves laminin and type IV collagen but causes the loss of some proteoglycan. It is concluded that, except for this loss and the absence of lamina lucida in cryofixed thin basement membranes, the morphological and antigenic features obtained after cryofixation are similar to those observed in the past after conventional fixation.
Anat Rec 1993 Feb
PMID:Cryofixation of basement membranes followed by freeze substitution or freeze drying demonstrates that they are composed of a tridimensional network of irregular cords. 842 Mar 89

The records of 14 cases of bovine hypokalaemia observed between 1983 and 1996 were reviewed. The most common history included a protracted, often infectious, disease. All age groups were represented. Although previously reported as a risk factor, isoflupredone acetate had not been administered to five of the cases. The following clinical signs were recorded in 10 cases: abnormal position of the head and neck, severe weakness, rumen hypomotility or atony, abnormal faeces, anorexia and tachycardia. Cardiac dysrhythmia was observed in six cases. Acid-base imbalance (alkalosis in 10 cases), hyperglycaemia and increased activities of aspartate aminotransferase and creatine kinase were associated with hypokalaemia ranging from 1.35 to 2.49 mmol/litre. Treatments included symptomatic treatment, supportive care and potassium chloride given intravenously and orally at an average total daily dose of 42 g/100 kg bodyweight (26 g by mouth and 16 g intravenously) for an average of five days. Eleven cases recovered after an average of three days.
Vet Rec 1998 Oct 31
PMID:Description of 14 cases of bovine hypokalaemia syndrome. 983 71

Recombinant murine MRP14 (mMRP14) was produced in Escherichia coli using the pGEX expression system. The mass of fusion protein, by electrospray ionization-mass spectrometry (ESI/MS), was 39,213 Da which compares well with the theoretical mass (39,210.4 Da). Thrombin digestion of fusion protein was expected at a cloned thrombin consensus sequence (. LVPRGS. ) located between glutathione S-transferase and mMRP14. Analysis of products of digestion by C4 reverse-phase HPLC and SDS-PAGE/Western blotting revealed two immunoreactive cleavage products with molecular weights around 13, 000. Masses of the two proteins determined by ESI/MS were 13,062 and 11,919 Da. The larger product corresponded to the expected mass of recombinant mMRP14 (13,061.9 Da). Analysis of the protein sequence of recombinant mMRP14 revealed a thrombin-like consensus sequence (. NNPRGH. ) located close to the C-terminus. The smaller protein corresponded to a truncated form of rec mMRP14 (rec MRP141-102) with a calculated mass of 11,918.6 Da. Optimization of the cleavage conditions resulted in >95% full-length rec mMRP14. Native mMRP14 contains one intramolecular disulfide bond between Cys79 and Cys90. The full-length recombinant protein was renatured and oxidized in ammonium acetate (pH approximately 7) for 96 h and formed >95% of the native intramolecular disulfide-bonded form. MRP141-102 bound substantially less 65Zn2+ compared to native mMRP14 or rec mMRP14 after transfer to polyvinylidene difluoride and incubation with 65ZnCl2, implicating the His residues located within the C-terminal domain in Zn2+ binding.
 ...
PMID:Overexpression, oxidative refolding, and zinc binding of recombinant forms of the murine S100 protein MRP14 (S100A9). 1004 80

Superoxide anion production in neutrophils plays an important role in the microbicidal defense system in the body. In this study, isolated rat neutrophils were stimulated experimentally and examined by electron microscopy to determine the site of superoxide production and its subsequent translocation during different cell stimulation time periods. Blood and peritoneal neutrophils were incubated for periods of 5, 10, and 15 min with phorbol 12-myristate 13-acetate (PMA), N-formyl-Met-Leu-Phe (fMLP), and combinations of PMA and cytochalasin B (CB) and fMLP and CB. Ultracytochemical detection of O(2)(-) was performed with the 3, 3'-diaminobenzidine-manganese (DAB/Mn) cytochemical method and cationized ferritin (CF) particles were added to stimulation media to monitor endocytotic events that occurred during neutrophil stimulation. Unstimulated neutrophils were devoid of O(2)(-) activity in cytoplasmic granules and at the plasma membrane surface. After 5 min stimulations with PMA, PMA + CB, or fMLP + CB, electron-dense DAB/Mn reaction product was detected in small, centrally located tubular compartments within the neutrophils. CF particles which were added to the stimulation media became internalized in endocytotic vesicles after 5 min stimulation; these vesicles were devoid of O(2)(-) activity. At 10 min stimulation with PMA, O(2)(-)-positive granules subsequently fused with each other and translocated to sub-plasma membrane regions where they either contacted the plasma membrane or fused with CF-containing endocytotic vesicles. Little reaction product was observed on the surface of the neutrophils. Spectrophotometric comparison of the stimulatory effects of PMA, fMLP, and fMLP + CB revealed different rates and yields of O(2)(-) production. Results from this study suggest that the O(2)(-)-producing sites of rat neutrophils originate intracellularly and translocate to the plasma membrane surface following stimulation with PMA, PMA + CB, and fMLP + CB, but not with fMLP or CB alone. Furthermore, these compartments appear to possess the ability to fuse with endocytotic vesicles, a process that may be linked to intracellular microbicidal activity in circulating and tissue neutrophils.
Anat Rec 2000 02 01
PMID:Ultracytochemical study on the localization of superoxide producing sites in stimulated rat neutrophils. 1064 63

Eighty-nine cats and 38 dogs naturally infested with the ear mite Otodectes cynotis were randomly allocated into two treatment groups. One group was treated with a product containing miconazole nitrate, polymyxin B sulphate and prednisolone acetate, the other with a combination of diethanolamine fusidate, framycetin sulphate, nystatin and prednisolone. The treatment (five drops in each ear) was applied twice daily for 14 days, and its efficacy was evaluated on days 7, 14 and 21 on the basis of an otoscopic examination of the external ear canal, a microscopical examination of scrapings for the presence of ear mites and clinical signs of pruritus, pain, erythema and/or exudate. Both treatments were highly effective, and there were no significant differences between the two products, either in efficacy or in the clinical improvements observed. Apart from an allergic reaction in one cat treated with the second product, no adverse effects were observed.
Vet Rec 2000 Nov 11
PMID:Efficacy of non-acaricidal containing otic preparations in the treatment of otoacariasis in dogs and cats. 1110 40


<< Previous 1 2 3 4 5 6 7 Next >>