Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q9UIJ5 (Rec)
 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Azaserine causes DNA damage in stationary-phase cells. In our investigation of this damage, we used strains of Escherichia coli differing in repair capabilities to study azaserine-induced DNA damage, detected as DNA strand breaks by sucrose gradient sedimentation techniques. Reduced sedimentation in alkaline and neutral sucrose gradients indicated the presence of both alkali-labile sites and in situ strand breaks. Azaserine induced DNA single-strand breaks (SSBs) abundantly in all but the recA strain, in which SSBs were greatly reduced. Treatment of purified DNA with azaserine from bacteriophages T4 and PM2 produced no detectable SSBs. Several other studies also failed to detect DNA damage induced directly by azaserine. Increased levels of beta-galactosidase were induced in an E. coli strain possessing a rec::lac fusion, providing further evidence for azaserine induction of the recA gene product. In addition, azaserine induced adaptation against killing but not against mutagenesis in wild-type E. coli strain.
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PMID:Azaserine: further evidence for DNA damage in Escherichia coli. 639 Jan 89

Azaserine in an antineoplastic agent, mutagen and carcinogen that is known to inhibit purine metabolism. Comparison of mutation in stationary-phase cultures of E. coli WP2 and a series of its DNA repair-deficient mutants exposed to azaserine showed that the effects of the compound closely mimicked those caused by UV light indicating, therefore, that azaserine-induced mutagenesis occurred via pathways dependent upon the recA and lexA genes. Comparison of survival of these strains showed that potentially lethal DNA lesions induced by azaserine were corrected by the excision, recombination, and rec-lex repair systems. These results show that azaserine causes DNa damage as well as inhibition of purine metabolism.
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PMID:Azaserine: survival and mutation in Escherichia coli. 704 85