Gene/Protein Disease Symptom Drug Enzyme Compound
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Isoenzymes of rat ventral prostate (RVP) acid phosphatase were isolated and partially purified by ultracentrifugation, Sephadex G-100 column chromatography, and isoelectric focusing. Antisera were raised to the isoenzymes of prostatic acid phosphatase by immunization of New Zealand white rabbits. Rabbit antisera reacting specifically to homologous but not heterologous isoenzymes of acid phosphatase were then reacted with a variety of tissues using indirect immunofluorescence. The tissues included prostate, spleen, bone marrow, liver, kidney, salivary gland complex, small intestine, and adrenal glands. An antiserum against a RVP acid phosphatase isoenzyme with a pI of 4.5 (A-PAP) localized acid phosphatase only in the supranuclear region of rat ventral prostate epithelial cells, and did not react with acid phosphatase in any of the other organs tested. A-PAP did not localize acid phosphatase in the ventral prostate from rats 14 days after castration. A-PAP did localize acid phosphatase in the ventral prostate from castrated animals that were treated with testosterone. These results indicate the A-PAP localized an androgen-dependent isoenzyme of acid phosphatase in RVP epithelial cells that may be secretory in nature. This antiserum should prove to be an ideal marker for studies involving hormonal regulation of prostatic epithelial function in vivo and in vitro.
Anat Rec 1985 Oct
PMID:Immunofluorescent localization of an androgen-dependent isoenzyme of prostatic acid phosphatase in rat ventral prostate. 390 18

A discrete population of cells containing immunoreactive somatostatin was shown by the peroxidase antibody bridge technique at both the light and electron microscopic level to be present in the pancreas of chick embryos. Based on the stage of development, somatostatin-positive cells, at the light microscopic level, were found in the alpha and beta islets, in isolated islets that did not correspond with any known alpha or beta islets, as well as in the acinar tissue. Quantitative determination indicated that, at all ages examined, there were a greater number of somatostatin cells associated with the alpha islets per square millimeter of tissue than with either the beta islets or acinar tissue of the exocrine pancreas. Ultrastructural observations on day 15 of development confirm and reinforce the light microscopic observations pertaining to the localization of the somatostatin-positive cells. The results also show that the PAP reaction was localized only on the granules of the somatostatin positive cells. These granules were primarily found in the apical region of these cells adjacent to blood vessels.
Anat Rec 1982 Mar
PMID:Light and electron microscopic immunohistochemical localization of somatostatin in the endocrine and exocrine portions of the pancreas of the chick embryo (Gallus domesticus). 612 88

S-100 protein, isolated from mammalian brain, has widely been used for immunohistochemical marker of the glia cells and the cells derived from the neural crest. In the present study, we made anti S-100 protein antibody and studied the immunoreactive distribution of S-100 protein in the cutaneous nervous system. Albino rabbits were immunized with S-100 protein and complete Freund adjuvant, and antiserum was purified by ion-exchange chromatography. Formalin-fixed normal human skin and sciatic nerve of rat were examined by the PAP method. S-100 protein was detected in Schwann cells of sciatic nerve of rat and cutaneous nerve bundles of human skin specimens. Meissner corpuscles and inner core cells of Pacinian corpuscles of human skin were S-100 protein positive. These findings suggest that the staining of S-100 protein with PAP method is a simple and reliable method to demonstrate the cutaneous nervous system. Also, lamellar cells of Meissner corpuscles and inner core cells of Pacinian corpuscles are indicated to be Schwann cell origin.
Anat Rec 1984 Dec
PMID:Immunohistological demonstration of S-100 protein in the cutaneous nervous system. 652 1