Gene/Protein Disease Symptom Drug Enzyme Compound
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 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The distribution of complex carbohydrates has been investigated cytochemically at the light and electron microscope levels in collecting ducts of the guinea pig kidney. The dialyzed iron method demonstrated acidic complex carbohydrate ultrastructurally on the outer surface of the apical and the basolateral plasmalemma of the principal cells and in their maturing Golgi cisternae and secretory granules. Glycoconjugate in these sites stained for sulfate esters with the high iron diamine method but lacked reactivity toward the periodic acid-thiocarbohydrazide-silver proteinate (PA-T-SP) sequence for visualizing vic glycol-containing glycoprotein. Lability to testicular hyaluronidase and resistance to sialidase identified the Glycosaminoglycan (GAG) in principal cell granules and the plasmalemmae as a chondroitin sulfate. In contrast, intercalated cells of the collecting ducts failed to stain with the cationic reagents, but showed light PA-T-SP reactivity demonstrative of neutral glycoprotein in the glycocalyx of the apical plasmalemma. Immunostaining with the immunoglobulin-enzyme bridge procedure localized carbonic anhydrase selectively to the intercalated cells. The ultrastructural and cytochemical observations on the guinea pig collecting ducts implicate intercalated cells in fluid and electrolyte transport and principal cells in secretion of a chondroitin sulfate to the tubule lumen and intercellular space.
Anat Rec 1982 Apr
PMID:Cell specialization in collecting tubules of the guinea pig kidney: carbonic anhydrase activity and glycosaminoglycan production in different cells. 680 16

The decidual reaction in mice is characterized by the transformation of a specific population of endometrial fibroblasts into epithelioid cells, known as decidual cells. An important feature of decidualization in mice is a remarkable modification of the endometrial extracellular matrix. The present work is an ultrastructural cytochemical study of matrix with the purpose of analyzing the arrangement of collagen-associated proteoglycans (PGs) at various regions of nulliparous endometrium and of the antimesometrial decidua of mice using the cationic dye cuprolinic blue associated with enzymatic treatments with chondroitinase ABC, chondroitinase AC, and hyaluronidase. The staining with cuprolinic blue showed PGs as rods and granules of several sizes. Rods measuring 40-60 nm in length (named F2-rods) were apposed to thin collagen fibrils whereas granules were associated with thick collagen fibrils, particularly in the region occupied by mature decidual cells on the 7th day of pregnancy. The amount of granules was higher than that of F2-rods. Both F2-rods and granules were affected by chondroitinase ABC or AC treatment, indicating that they were PGs containing chondroitin sulfate and dermatan sulfate chains. However, the granules associated with thick collagen fibrils were more resistant to chondroitinase AC treatment than F2-rods, indicating the presence of dermatan sulfate chains that contain both L-iduronic and D-glucuronic acid sugar residues. We suggest that the differences of the nature and amount of PGs may be associated with the changes of the thickness of collagen fibrils observed during decidualization of the endometrium in the mouse.
Anat Rec 2000 08 01
PMID:Ultrastructural cytochemical characterization of collagen-associated proteoglycans in the endometrium of mice. 1090 33


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