Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:Q9UIJ5 (Rec)
 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Buffalo (Bos bubalis) lymphocytes were purified and tested for their E and EAC rosette forming capacity as a marker for the detection of T and B cells, respectively. Sheep erythrocytes were found to form 17.7 per cent of E rosette with buffalo lymphocytes. This population of lymphocytes is believed to be T cell. Erythrocytes of guinea-pig, rabbit, hamster, rat, chicken, dog and donkey formed a lower percentage of rosettes. Five to 18.5 per cent of SRBC-EAC rosettes were detected with buffalo lymphocytes which are believed to be B cells.
Vet Rec 1979 Apr 28
PMID:Rosette formation by buffalo (Bos bubalis) T and B lymphocytes. 31 94

A T-cell replacing factor (TRF)/interleukin-5 (IL-5) is a B-cell growth and differentiation factor. In the present study, we examined the role of TRF/IL-5 in the increase in the levels of interleukin-2 (IL-2) receptor expression on activated B-cells. High pressure liquid chromatography (HPLC)-purified TRF/IL-5 (B151-TRF) from TRF-producing T-cell hybridoma, B151K12, as well as recombinant TRF/IL-5 (rec-TRF) were used for the analysis. Maximum anti-2,4-dinitrophenyl (DNP) IgG antibody response of DNP-primed B-cells or polyclonal IgM secretion of B-cell tumor line BCL1 was seen when HPLC-purified B151-TRF was added or when suboptimal doses of B151-TRF were added to the culture in the presence of IL-2. Normal resting B-cells gave maximum anti-SRBC IgM PFC responses when HPLC-purified B151-TRF and IL-2 were present. The purified B151-TRF as well as rec-TRF also induced on B-cells increased expression of IL-2 receptors that react with monoclonal anti-murine IL-2 receptor antibody, PC61, and 125I-labelled IL-2. The numbers of functional high affinity IL-2 receptors on activated B cells increased at least 20-fold by culturing them with purified B151-TRF. Moreover, B151-TRF induced increase in the levels of steady-state mRNA for IL-2 receptor by approximately 8-fold. These results suggest that activated B-cells as well as BCL1-cells may express functional IL-2 receptors or closely related molecules when stimulated with HPLC-purified B151-TRF as well as rec-TRF.
 ...
PMID:T cell replacing factor/interleukin 5 induces not only B-cell growth and differentiation, but also increased expression of interleukin 2 receptor on activated B-cells. 311 78

In this study the effect of recombinant human interleukin 2 (rec.hIL-2) on the proliferation and maturation of B lymphocytes was investigated. It was found that the presence of rec.hIL 2 results in proliferation of mitogen (LPS)-activated B cell blasts. In addition, it is shown that highly enriched murine B cells can be induced by rec.hIL-2 to proliferate and to develop into antibody-secreting cells (PFC) in the presence of antigen (SRBC). When tested for its effect on B cell preparations enriched for resting (small) or activated (blasted) B lymphocytes, it was found that rec.hIL 2 provides signals for both B cell populations to develop into PFC. In contrast, induction of proliferation by the same lymphokine source was only seen in blasted B cells. The data indicate that IL 2 is involved in the generation of B effector cells by directly acting on their precursors thereby providing differentiation as well as proliferation signals.
 ...
PMID:Recombinant human interleukin 2 directly provides signals for the proliferation and functional maturation of murine B lymphocytes. 387 48

In order to identify the initial site of antibody formation in rat spleen, an investigation was made to determine the effects of different antigen dosages on the localization of specific antibodies against sheep erythrocytes (SRBCs). Sixty rats were intravenously injected with 1 ml of either 1%, 5%, or 10% suspensions of SRBCs and killed at days 1, 2, 3, and 4 after immunization. A tissue agglutination procedure in which the binding of SRBCs to cryostat sections of spleen was used to localized anti-SRBC antibodies. Sections used for determination of SRBC binding patterns, and adjacent sections were stained for histological localization or processed for the determination of acid phosphatase (ACP) activity. Spleens of non-immunized rats showing binding of SRBCs closely associated with the ACP-positive marginal metalophils and marginal zone macrophages. This binding was not inhibited by preincubating the sections with 2-mercaptoethanol. The bound SRBCs lysed when incubated with complement. The initial change that occurred after antigen injection was binding over the germinal was not associated with ACP-positive cells. In animals immunized with 1% SRBCs, these changes were seen on the third day after immunization. In animals immunized with 1% SRBCs, these changes were seen on the third day after immunization. In animals immunized with 5% or 10% suspensions of SRBCs, these changes occurred 24 hours after immunization, during dissociation of the germinal centers. In later stages there was heavy binding of SRBCs over the white pulp and over the red pulp. Binding induced by immunization was inhibited by pretreating the sections with 2-mercaptoethanol and the bound cells lysed in the presence of complement. The results obtained suggest that IgM antibody to SRBCs appears in the germinal centers at least as early as in the marginal zone or peripheral periarterial region, and support the view that germinal centers may participate in primary antibody responses.
Anat Rec 1980 Nov
PMID:Effects of antigen dosage on early localization of specific antibodies in rat splenic germinal centers. 745 40