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 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Collagen fibrils were present within membrane-bound vacuoles in the cytoplasm of mouse decidual cells on the 7th day of pregnancy. The space between the vacuole membranes and the fibrils was narrow and frequently filled with a granular electron-dense material. The loss of banding of the collagen fibrils, their association with lysosomelike bodies, and the demonstration of acid phosphatase activity in the vacuoles indicate that the fibrils were internalized by the decidual cells and were being digested. It is suggested that phagocytosis of collagen is a mechanism of remodeling of the mouse decidua.
Anat Rec 1989 Oct
PMID:Phagocytosis of collagen by mouse decidual cells. 281 34

After fetal lungs are placed in organ culture, any macrophages arising in them must develop from precursors present at explantation. Whole pseudoglandular lungs from 20 litters of rats were set on an agar medium containing fetal bovine serum on the 14th prenatal day when the metamorphosing embryos do not yet have circulating monocytes. After a day in vitro, 10-30-micron clusters of rounded cells began to appear in the pulmonary connective tissue. They increased in size, displayed mitotic figures, and were supplemented by additional clusters on succeeding days. Late in the second day, cells began to penetrate the visceral pleura and over the next week built up a large population outside. Histochemically, the intrapulmonary foci and emerging cells were reactive for naphthyl acetate esterase and acid phosphatase, and many were stainable for triglyceride. Within the lungs elevated acid phosphatase activity was first seen at 24 hours; it attained the level of activated alveolar macrophages 1-2 days later. After cells had been emerging 4-5 days, comparatively few clusters remained in the stroma, but some cells had crossed into the airway, to be swept along by ciliary currents. Some cultures were injected with submicronic iron oxide particles on the first day. The particles gradually concentrated within the stromal clusters, and a few emerging cells contained them. Emerged cells avidly ingested iron oxide and rhodamine-coated latex microspheres. Cells adhering to the cultures were centers of rosette formation after exposure to sheep erythrocytes opsonized with rabbit antisheep IgG and complement factors. The explants evidently contained precursors of macrophages. Cultural conditions provoked them into dividing and exhibiting activation of lysosomes and capacity for directed migration; our experiments showed the transformed cells to be phagocytic and to have receptors associated with immune phagocytosis. The study indicates that macrophages directly derived from stem cells in embryonic lungs are similar in essentials to macrophages in adult lungs, and that this component of pulmonary immune defense is present in an occult form from almost the outset of development.
Anat Rec 1987 Jan
PMID:Pure population of nonmonocyte derived macrophages arising in organ cultures of embryonic rat lungs. 345 63

Human NK activity is known to be associated with a population of large granular lymphocytes (LGL) exhibiting several immunophenotypic surface markers including Leu-11a (NKP-15), Leu-7 (HNK-1), Leu-3a (T4), and Leu-2a (T8). Based upon correlation with cytolytic activity, Leu-11a is now considered the most specific antigenic marker for human NK cells. Present investigation compared the ultrastructure of cells expressing Leu-11a, Leu-7, Leu-3a, and Leu-2a, both in human peripheral blood lymphocytes (PBL) and the purified LGL fraction. Subcellular cytochemical reactions were investigated in Leu-7+ or Leu-11a+ PBL or LGL and in cells conjugated with K562 targets (indicating NK cytolytic potential). The surface markers, localized with monoclonal antibodies, were detected by immunoelectron microscopy by using direct or indirect avidin-biotin-peroxidase (ABC) or colloidal gold methods. A peroxidase-colloidal gold double-labeling system was used to identify subsets of Leu-7+ or Leu-11a+ cells. Previously described ultrastructural features of LGL including a villous surface, reniform nuclei, low nuclear/cytoplasm ratios, and abundant cytoplasm with vesicles, vacuoles, electron-dense granules, parallel tubular arrays (PTA), or paracrystalline inclusions were associated with Leu-7+, Leu-11a+, Leu-7+/Leu-11a+, Leu-7+/Leu-11a-, and Leu-7-/Leu-11a+ PBL or LGL. Results showed that the Leu-7+/Leu-11a+ cells were the most abundant NK cells in PBL. Lymphocyte subsets with Leu-3a or Leu-2a surface marker showed some ultrastructural features including PTA similar to Leu-7+ cells and Leu-11a+ cells, and their subsets. These T-cells appeared ultrastructurally more similar to the Leu-7+/Leu-11a- subset. Cytochemical studies showed that electron-dense cytoplasmic granules and PTA typical of the Leu-11a+ cells and Leu-7+ cells contained glycoprotein, acid phosphatase, and arylsulfatase. Large cytoplasmic vacuoles were heterogeneous and typically contained electron-dense material with DAB reactivity, membranous material, PTA, and/or paracrystalline inclusions. Glycoprotein, acid phosphatase, and arylsulfatase, and peroxidase reactive material were also found in these vacuoles. These features suggested that the vacuoles could be secondary lysosomes. The coexistence of intact PTA or degenerating PTA in the same vacuoles with paracrystalline inclusions suggested that the latter are possibly derived from PTA.
Anat Rec 1987 Mar
PMID:Immunoultrastructural studies of human NK cells: I. Ultracytochemistry and comparison with T cell subsets. 355 61

A light and electron microscope study of the small intestine of the little brown bat, Myotis lucifugus, was carried out at several stages in the animal's annual life cycle. An unusual morphological observation was the presence of cells in the lamina propria of the small intestine which were packed with a conspicuous basophilic granular material that appeared crystalline. Moreover, such cells were present only during the hibernation period and were therefore called "hibernation crystalloid" (HC) cells. By light microscopy, the crystal-like material was not sudanophilic, did not stain for nucleic acids, and did not contain acid phosphatase; it did show reactivity when stained by the periodic acid-Schiff procedure. By electron microscopy, the crystal-like material was found to be present in smooth, membrane-enclosed vacuoles along with an amorphous, dense granular substance. The crystalline material occasionally formed rigid-appearing rods that reached lengths of 10 microns. The crystal-containing cells were contacted by axonal varicosities. It is suggested that these innervated HC cells represent a unique cell type with a gastrointestinal function, yet to be determined, that may be related to hibernation.
Anat Rec 1987 Jun
PMID:Occurrence of cells containing paracrystalloid material in the intestinal lamina propria of the hibernating bat Myotis lucifugus. 361 83

Sympathectomy was carried out in 4-week-old Sprague-Dawley rats by unilateral surgical removal of the superior cervical ganglion. Sham-treated rats served as controls. All rats were injected with tetracycline hydrochloride at surgery as well as 36 hr prior to sacrifice. Rats were killed at 7, 14, or 21 days following sympathectomy. Mandibular periosteal and endosteal surfaces were analyzed by fluorochrome morphometry. Osteoclasts were identified by acid phosphatase staining, and incisor and molar root sockets were analyzed morphometrically. Following sympathectomy, periosteal and endosteal apposition as well as the rate of mineralization were significantly lower. At the same time, a significant increase in the number of osteoclasts per socket as well as in active and inactive bone resorption surfaces was also seen. All parameters, however, returned to normal values 2-3 weeks after sympathectomy. The data provide the first direct quantitative evidence that sympathetic neurons modulate bone resorption and bone remodeling in vivo.
Anat Rec 1987 Sep
PMID:Effect of surgical sympathectomy on bone remodeling at rat incisor and molar root sockets. 368 59

Osteoclast progenitors are seeded via the blood stream in the mesenchyme surrounding embryonic long bone models long before the appearance of multinucleated osteoclasts. The proliferation and differentiation of these progenitors in embryonic mouse metatarsal bones was studied with acid phosphatase (AcP) histochemistry and 3H-thymidine autoradiography. In vivo, tartrate-resistant, acid phosphatase-positive, mononuclear cells appear in the periosteum (AcPP-P cells) at the age of 17 days (after conception). On day 18, AcP-positive, multinucleated osteoclasts invade the bone rudiment and start resorbing the calcified cartilage matrix, resulting in the formation of the marrow cavity. The kinetics of osteoclast formation in vitro was studied in metatarsal bones of embryonic mice of different ages cultured in the continuous presence of 3H-thymidine. In young bones (15 days), mainly proliferating, 3H-thymidine-incorporating progenitors gave rise to AcPP-P cell and osteoclast formation. In older bones (16 and 17 days) osteoclasts were progressively more derived from postmitotic, unlabeled precursors. Irradiation of the metatarsal bones with a radiation dose of 5.0 Gy prior to culture resulted in a selective elimination of the proliferating progenitors, whereas the contribution of postmitotic precursors in AcPP-P cell and osteoclast formation remained unchanged. The results demonstrate that in the periosteum of embryonic metatarsal bones a shift occurs from a population composed of proliferating osteoclast progenitors (15 days) to a population composed of postmitotic precursors (17 days) before multinucleated osteoclasts are formed (18 days). Obviously, postmitotic AcP-negative precursors, already present in 16-day-old bones, differentiate into precursors characterized by tartrate-resistant AcP activity, the preosteoclasts (17 days), which in their turn fuse into osteoclasts.
Anat Rec 1986 Apr
PMID:Differentiation kinetics of osteoclasts in the periosteum of embryonic bones in vivo and in vitro. 370 84

M cells are specialized epithelial cells over lymphoid follicles in Peyer's patches which take up viruses, bacteria, and antigenic macromolecules from the intestinal lumen. Unlike ordinary enterocytes which sequester pinocytosed material in lysosomes, M cells transport such material across the epithelium to antigen-processing areas in lymphoid follicle domes, suggesting a difference in lysosomal activity or a different route for movement of endocytic vesicles. Ileal Peyer's patches in rats were examined by electron microscopy to identify lysosomes by acid phosphatase activity. Acid phosphatase was found in dense bodies in enterocytes but not in M cells. Stereological analysis showed the volume fraction occupied by dense bodies in M cells to be 16 times less than in enterocytes (P less than .0005), even though the volume fractions of cytoplasm occupied by mitochondria in M cells and enterocytes were not significantly different. The small volume fraction of dense bodies and the absence of acid phosphatase activity in M cells thus correlate with absence of lysosomal degradation of luminal microorganisms during transport into lymphoid follicles by M cells and may provide not only a complete array of microbial antigens for initiation of immune responses, but also a route through the mucosal barrier for microorganisms which can evade local containment mechanisms.
Anat Rec 1986 Dec
PMID:Morphometric and cytochemical analysis of lysosomes in rat Peyer's patch follicle epithelium: their reduction in volume fraction and acid phosphatase content in M cells compared to adjacent enterocytes. 379 99

Isoenzymes of rat ventral prostate (RVP) acid phosphatase were isolated and partially purified by ultracentrifugation, Sephadex G-100 column chromatography, and isoelectric focusing. Antisera were raised to the isoenzymes of prostatic acid phosphatase by immunization of New Zealand white rabbits. Rabbit antisera reacting specifically to homologous but not heterologous isoenzymes of acid phosphatase were then reacted with a variety of tissues using indirect immunofluorescence. The tissues included prostate, spleen, bone marrow, liver, kidney, salivary gland complex, small intestine, and adrenal glands. An antiserum against a RVP acid phosphatase isoenzyme with a pI of 4.5 (A-PAP) localized acid phosphatase only in the supranuclear region of rat ventral prostate epithelial cells, and did not react with acid phosphatase in any of the other organs tested. A-PAP did not localize acid phosphatase in the ventral prostate from rats 14 days after castration. A-PAP did localize acid phosphatase in the ventral prostate from castrated animals that were treated with testosterone. These results indicate the A-PAP localized an androgen-dependent isoenzyme of acid phosphatase in RVP epithelial cells that may be secretory in nature. This antiserum should prove to be an ideal marker for studies involving hormonal regulation of prostatic epithelial function in vivo and in vitro.
Anat Rec 1985 Oct
PMID:Immunofluorescent localization of an androgen-dependent isoenzyme of prostatic acid phosphatase in rat ventral prostate. 390 18

Following subtotal thyroidectomy, the amount of circulating thyroid hormone decreases and causes an increase in the secretion of thyrotropin (TSH) by the anterior pituitary gland. Serum levels of circulating TSH remain elevated until thyroid secretion returns to normal. In this study we have analyzed the effects of such chronic stimulation of thyroid cells by TSH, with particular emphasis on ultrastructural and cytochemical changes in the lysosomes. Weanling Sprague-Dawley rats underwent subtotal thyroidectomy and 6 weeks later the residual thyroid tissue was removed and processed for ultrastructural and cytochemical analysis. There were obvious ultrastructural signs of hyperactivity. The cells were hypertrophied and there were colloid droplets in the cells as well as extremely abundant oddly shaped lysosomes. The lysosomes reacted positively for acid phosphatase and for glycoproteins, suggesting that they are secondary lysosomes, ones which have complexed with thyroglobulin prior to release of thyroid hormones from the cells. This tremendous increase in the number of these structures in the cells is similar to that observed under normal conditions during the aging process and suggests a slowdown in the proteolytic degradation of thyroglobulin during long periods of chronic stimulation by TSH.
Anat Rec 1986 Feb
PMID:Ultrastructure and cytochemistry of thyroid lysosomes following subtotal thyroidectomy. 395 73

Ciliated cells of the ductuli efferentes show at their apex a discrete endocytic apparatus composed of small vesicles connected to or subjacent to the apical plasma membrane, small apical membranous tubules, and pale multivesicular bodies. Deeper in the cytoplasm, there are acid phosphatase-positive denser, multivesicular bodies and secondary lysosomes showing an electron-dense cortex and a crystalline, paler stained core. Cationic ferritin and concanavalin A-ferritin used to demonstrate adsorptive endocytosis, when injected into the rete testis, rapidly reached the lumen of the ductuli efferentes. At 1 min after injection, these tracers were seen bound to the apical plasma membrane of ciliated cells and within small endocytic vesicles and by 5 min in narrow apical tubules. At 15 and 30 min after injection, the tracers appeared in pale multivesicular bodies while at 1 hr they were found within dense multivesicular bodies. At 2 hr and longer time intervals these tracers accumulated within secondary lysosomes. Native ferritin, concanavalin A-ferritin in the presence of alpha-methyl-D-mannoside, and horseradish peroxidase or albumin-colloidal gold complexes were used to analyze fluid-phase endocytosis. At various intervals following their injection into the rete testis, these tracers presented a distribution identical in all respects to that described for cationic ferritin and concanavalin A-ferritin. In the present work, none of the above tracers were transported to the abluminal aspect of the ciliated cells. These cells, like the nonciliated epithelial cells of the ductuli efferentes are thus involved in adsorptive as well as in fluid-phase endocytosis.
Anat Rec 1985 Mar
PMID:Fluid-phase and adsorptive endocytosis in ciliated epithelial cells of the rat ductuli efferentes. 403 43


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