Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q9UIJ5 (Rec)
 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Transfection of Escherichia coli spheroplasts by native T5 phage DNA was not affected by treatment with polynucleotide ligase. Denatured T5 phage DNA infectivity, only 0.1% of the native DNA level, was increased slightly by polynucleotide ligase treatment. Renatured T5 phage DNA infectivity was also increased slightly by polynucleotide ligase treatment. To form an infective center with rec(+) spheroplasts, 1.6 to 2.1 native T5 phage DNA molecules were required; however, 1.4 T5 phage DNA molecules were required to form an infective center with recA(-)B(-) spheroplasts, and one molecule was sometimes sufficient for rec B(-) spheroplasts. Polynucleotide ligase treatment of T5 phage DNA had no effect on these parameters. Thus, the single-strand interruptions of T5 phage DNA are probably not essential to the survival of the parental T5 phage DNA, and T5 phage DNA, especially the denatured form, is highly sensitive to some nucleases in E. coli spheroplasts.
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PMID:Transfection of Escherichia coli spheroplasts. IV. Transfection of rec+ and rec minus spheroplasts by native, denatured, and renatured T5 bacteriophage DNA after repair of single-strand breaks by polynucleotide ligase. 459 99

We have isolated recombination deficient mutants of Bacillus subtilis on the basis of their sensitivity to methyl-methane-sulfonate or ultraviolet light, or of their inability to be transformed on solid medium. We have analyzed the mutants for several recombination and repair properties; we have grouped them in 5 classes on the basis of their phenotype and tested them for the activity of several enzymes acting on DNA, ie. DNA polymerase, polynucleotide ligase, ATP dependent DNase, and a DNase acting on single-stranded DNA. One mutant was found reduced in the latter DNase. Some of the mutants have been mapped, and they correspond to three different genes denominated rec D, rec F and rec G. All the recombination deficient mutants of B. subtilis described in the literature have been grouped in 7 classes; the mutations belong to 13 (and possibly 15) different genes distributed along the map. A coherent nomenclature and the criteria for a standard study of the rec mutants are proposed.
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PMID:Genetic and enzymic studies on the recombination process in Bacillus subtilis. 1609 63