Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:Q9UIJ5 (Rec)
 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An interlaboratory trial was conducted of a latex agglutination kit for the rapid identification of Salmonella enteritidis, to assess the stability of the components and its performance with respect to a panel of three coded salmonellas and 243 field isolates. Two of the components of the kit deteriorated with time. All 62 isolates of S enteritidis were correctly identified by the kit; only two false positives were recorded and no false negatives.
Vet Rec 1992 Sep 12
PMID:An interlaboratory trial of a latex agglutination kit for rapid identification of Salmonella enteritidis. 144 Nov 13

The performance of a new kinetic assay test kit for the determination of magnesium in plasma or serum has been evaluated, using bovine plasma samples. The results obtained were compared with the results from a standard atomic absorption spectrophotometry method and a commercial colorimetric test kit. The kinetic kit compared favourably with the conventional methods and generally gave results with greater precision.
Vet Rec 1991 Mar 16
PMID:Evaluation of a kinetic test kit for the determination of magnesium in bovine serum or plasma. 203 17

There is a need for a simple and accurate method for detecting pregnancy in rabbits; the available methods are not ideal and may not provide the diagnosis at an appropriate time for the early remating of non-pregnant animals. This paper describes the use of an ELISA kit to measure progesterone concentrations in rabbit plasma; a qualitative assessment of the results appears to be sufficiently accurate for pregnancy diagnosis, as does the use of serum instead of plasma. The technique can be used to predict ovulation and to distinguish between pregnant and pseudopregnant animals.
Vet Rec 1990 Nov 24
PMID:Use of an ELISA for plasma progesterone to facilitate rabbit husbandry. 228 86

Blood samples taken from the ear vein of 1037 sows two to three weeks after service were assayed the same day using Ovucheck 'Sowside' kits. Colour development was compared with oestrous and pregnancy controls. Reliable data on reproductive performance were obtained from 908 sows sampled 17 to 20 days after service. The accuracy of identification of 796 pregnant and 12 non-pregnant sows was 94.6 per cent and 35.7 per cent, respectively. Excluding animals which returned outside the normal range of 18 to 24 days, 52.1 per cent of 48 empty sows were identified by the test. Problems with blood sampling were reported on seven of 18 farms and this may explain the low accuracy of the kit on some farms.
Vet Rec 1989 Feb 04
PMID:Use of an on-farm progesterone assay kit to determine pregnancy in sows. 264 22

Plasma samples were obtained from seven bitches during 10 oestrous periods. Progestogen concentrations were determined in 182 samples by radioimmunoassay and by both quantitative and qualitative enzyme-linked immunoassay (ELISA) commercial kits. The concentrations determined by the quantitative ELISA were consistently higher than those measured by radioimmunoassay although the values were highly and significantly correlated. The qualitative ELISA readily distinguished between low and high progestogen concentrations, ie, less than 1.0 ng/ml and greater than 2.6 ng/ml measured by radioimmunoassay. Sequential evaluation with either kit, especially when combined with vaginal cytology, should be useful in determining the optimum time for mating.
Vet Rec 1989 Jul 29
PMID:A comparison of radioimmunoassay with quantitative and qualitative enzyme-linked immunoassay for plasma progestogen detection in bitches. 277 43

An amplified enzyme immunoassay kit for progesterone analysis was used to diagnose pregnancy in a flock of 130 mule ewes. An accuracy of 100 per cent was obtained after the analysis of progesterone in plasma samples taken 15 to 16 days after mating. In mule ewes a plasma progesterone level greater than 5.9 nmol/litre was indicative of pregnancy. In the validation of the technique, duplicate ewe plasma samples and progesterone standards were compared with a radio immunoassay technique; the regression coefficient between the two techniques was r = 0.82.
Vet Rec 1987 Jul 18
PMID:Assessment of pregnancy in sheep by analysis of plasma progesterone using an amplified enzyme immunoassay technique. 330 17

A commerical kit designed to measure the concentration of progesterone in bovine plasma using an enzyme-linked immunosorbent assay (ELISA) has been assessed for measuring progesterone in the plasma of horses, sheep and dogs. Without validation, an immunoassay developed for progesterone in one species should not be used to measure progesterone in the plasma of other species. The kit was assessed by using the criteria of parallelism to a standard curve, the recovery of added progesterone, the correlation with an established radioimmunoassay and the detection of physiological change for each of the species tested. The ELISA kit proved to be acceptable for the analysis of progesterone in the plasma of each species, and in particular for the detection of the low concentrations found during the follicular phase and of the subsequent rise during the luteal phase of the oestrous cycle.
Vet Rec 1987 Jan 03
PMID:The use of a bovine plasma progesterone ELISA kit to measure progesterone in equine, ovine and canine plasmas. 381 Nov 97

The commercially available test kit for assaying milk progesterone was used in the practice laboratory on samples taken daily from cows 17 to 24 days after service. Improved oestrus detection rates and accuracy were achieved by predicting the onset of oestrus. Similar results were obtained by sampling on days 18, 20, 22 and 24 or on days 19, 21 and 23 after service. Calving to conception intervals improved from 115 to 84 days in one herd and from 85 to 74 days in another and the potential economic benefits in these two herds outweighed the costs by 7.4:1 and 3.4:1, respectively.
Vet Rec 1987 Jan 10
PMID:Oestrus prediction in dairy cows using an ELISA progesterone test. 382 30

Commercial radioimmunoassay kits for the measurement of human total thyroxine (T4) and total triiodothyronine (T3) based on magnetic separation are discussed for use in dogs and cats. The T4 assay procedure is modified to increase the accuracy over normal canine and feline ranges using four standards from kit components provided. Results of validation are presented and indicative normal ranges given: canine T4, 0.94 to 3.45 micrograms/dl; canine T3, 0.36 to 1.52 ng/ml; feline T4, 1.34 to 4.70 micrograms/dl; feline T3, 0.23 to 0.95 ng/ml. There is a significant breed difference in T4 levels between poodles and Yorkshire terriers.
Vet Rec 1984 Jul 28
PMID:Magnetic antibody immunoassay thyroid function tests in general practice. 647 79

An ELISA kit for measuring plasma progesterone in bitches was investigated to determine its potential as an assay for rabbit progesterone. The results suggest that the kit can be used for the qualitative assessment of rabbit progesterone, either in plasma or whole blood. Pregnant and pseudopregnant rabbits can be differentiated accurately at day 19 which is reported to be the optimum time to remate pseudopregnant females. The fact that whole blood can be used instead of plasma offers considerable potential benefit to rabbit breeders.
Vet Rec 1993 Apr 24
PMID:Preliminary investigation of an ELISA kit as a qualitative assay for rabbit progesterone. 849 2


1 2 3 4 5 Next >>