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 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mammary tumour tissue from two bitches and an anal adenoma from a dog were investigated for steroid receptor interaction. Both mammary tumours possessed cytoplasmic macromolecules sedimenting with coefficients of 4S and 8S that bound oestradiol-17beta. These receptors had molecular weights of approximately 60,000 and 180,000 respectively. Transfer of the oestrogen to the nucleus was shown and the presence of a 4-5S nuclear protein demonstrated. The anal adenoma had a cytoplasmic receptor, with a sedimentation value in a sucrose density gradient of 4-5S with respect to bovine serum albumin, that bound tritiated 5alpha-androstane-3alpha, 17alpha-diol. No affinity could be demonstrated for other C19-steroids examined. The significance of these findings in terms of the hormone dependence of the tumours investigated and the possible development of these studies to promote rational therapy in such cases is discussed.
Vet Rec 1975 Dec 13
PMID:Tissue-steroid interactions in canine hormone-dependent tumours. 17 72

The relative effectiveness of implanting 300 mg trenbolone acetate alone or in combination with either 15, 30 or 45 mg hexoestrol was studied in three 90-day experiments using 64 Friesian steers. In experiment 1 hexoestrol was shown to improve live-weight gain and efficiency of feed conversion in steers implanted with trenbolone acetate. In experiments 2 and 3 trenbolone acetate in combination with 30 mg hexoestrol gave a better growth response than when combined with either 15 or 45 mg. However, in experiment 3 trenbolone acetate plus 15 mg hexoestrol was shown to improve live-weight gain by about 36 per cent compared with untreated controls. In experiment 2 small differences between treated groups in mean values for plasma urea, serum albumin, plasma glucose and free fatty acids were recorded.
Vet Rec 1979 Sep 22
PMID:Effect of hexoestrol on the response of finishing steers to treatment with trenbolone acetate. 51 14

The toxicity of xanthene dyes were studied by various interaction between the dyes and the components in vital body. (1) An increase in the amount of Rose Bengale adsorbed on the gill of fish was followed by the increase in red corpuscle number, and it was assumed that the death of fish in xanthene dye solution was due to anoxemia. (2) Binding capacity of xanthene dyes with bovine serum albumin decreased in the following; Rose Bengale, Phloxine, Erythrosine, Eosine and Uranine. This order was quite coincident with the toxicity compared by TLm values. (3) From the results of rec-assay test by use of Bacillus subtilis, it was confirmed that Phloxine and Rose Bengale had DNA-damaging capacity related to the mutagenecity.
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PMID:Studies on the toxicity of coal-tar dyes II. Examination of the biological reaction of coal-tar dyes to vital body. 53 26

Twenty British Friesian steers were divided into four uniform groups and either not treated or implanted with hexoestrol, trenbolone acetate, or hexoestrol plus trenbolone acetate. Hexoestrol was given 90 days and trenbolone acetate 70 days, before slaughter. Animals in the treatment groups grew significantly faster, converted food to live-weight gain more effciently faster, converted food to live-weight gain more efficiently and had lower levels of plasma urea and to a lesser extent serum albumin than untreated controls for the final 70 days before slaughter. The combined treatment of hexoestrol plus trenbolone acetate produced more pronounced effects than either compound given alone. Steers treated with hexoestrol had significantly greater levels of serum growth hormone than steers implanted with trenbolone acetate alone or untreated controls, but the treatments had no significant effect on levels of plasma glucose, free fatty acids or serum insulin. Carcase conformation and fat cover assessed subjectively did not differ between treated and control animals but killing out percentage was generally higher in all treatment groups.
Vet Rec 1978 Jul 08
PMID:Performance, blood and carcase characteristics of finishing steers treated with trenbolone acetate and hexoestrol. 68 99

This paper reports adsorptive endocytosis of exogenous proteins by the trophotaenial absorptive cells (TACs) in the viviparous goodeid teleost, Ameca splendens. In vitro incubations were performed with gold conjugated to bovine serum albumin (Au-BSA), human transferrin (Au-HTf), fetuin (Au-Fet), and asialofetuin (Au-ASFet). Localization of gold label on the TAC surface was nearly exclusive to patches of an amorphous coat associated with part of the intermicrovillous plasma membrane. On addition of excess BSA, HTf, Fet, or ASFet to incubation media containing, respectively, Au-BSA, Au-HTf, Au-Fet, or Au-ASFet, the density of gold particles adsorbed on the TAC surface decreased drastically. Moreover, attachment of the four protein-gold complexes to the same plasma membrane sites was suggested by reciprocal inhibitory effects. Further proteins such as hemoglobin, myoglobin, and cytochrome c were as well potent inhibitors of Au-BSA and Au-HTf binding and uptake. Binding of TACs of native BSA or HTf was visualized by immunogold labeling. The interactions between proteins and binding sites required both the presence of Ca2+ and appropriate pH greater than 6.6. Analyses of the concentration-dependent BSA and HTf binding curves, plotted from morphometric data, resulted in apparent dissociation constants, Kds, of approximately 5 x 10(-7) M and 4 x 10(-7) M, respectively. Following binding at the TAC surface and internalization via clathrin-coated pits and vesicles the several ligands were routed along the lysosomal pathway with transit through the endosomal compartment. Prolonged incubation periods led to massive intracellular accumulation of tracer proteins. The effects of NH4Cl (10 mM) treatment on TACs included enormous cytoplasmic vacuolation, a reversible loss of protein binding sites on the plasma membrane, and a block in the transport of protein-gold complexes to lysosomes.
Anat Rec 1992 Jul
PMID:Protein-gold transport in the endocytic complex of trophotaenial absorptive cells in the embryos of a goodeid teleost. 160 71

Serum calcium, magnesium and phosphate values of ewes recently affected by vaginal prolapse were compared with unaffected ewes in four flocks. Subclinical hypocalcaemia was demonstrated in some affected and unaffected ewes in three flocks. Magnesium and phosphate values were normal. In two flocks the body condition of ewes recently affected by vaginal prolapse was variable and reflected the variation in condition found in the flock. In a third flock affected ewes had significantly lower body condition scores than unaffected ewes (P less than 0.001). Analysis of the fourth flock was not possible. Oestrogenic mycotoxins were not detected in any of the feed samples taken from these flocks. The following year the management, nutrition and energy, and the protein and calcium status of ewes in 12 flocks of greyface/mule ewes with a history of a regular high (greater than 3 per cent) or low (less than 1 per cent) prevalence of vaginal prolapse were compared. A high prevalence was not associated with any particular feedstuff. A high or intermediate (1 to 3 per cent) prevalence of vaginal prolapse was found in three of the four flocks managed as a single group and these three flocks were fed on an unrestricted basis. Body condition scoring and beta-hydroxybutyrate estimation confirmed that ewes in these flocks were overfed. The prevalence of vaginal prolapse in the flocks was not related to the serum albumin, calcium or urea of the ewes. Therefore subclinical hypocalcaemia was probably a consequence of vaginal prolapse rather than a cause.
Vet Rec 1991 Mar 02
PMID:Nutritional factors associated with vaginal prolapse in ewes. 203 22

Although tingible body macrophages (TBM) have been recognized in germinal centers for over 100 years, their role in the germinal center response is not clear. In this study, the kinetics of the TBM response was quantitatively assessed and correlated with the kinetics of germinal center development in young mice. The TBM response in old mice (which have an age-related depression of germinal center development; Szakal et al., 1990) was analyzed for comparison. Young and old immune mice were challenged with human serum albumin and 0, 1, 3, 5, 7, 10, and 14 days later the popliteal and axillary lymph nodes were evaluated. Germinal centers were localized histochemically in alternate serial sections using horseradish peroxidase conjugated peanut agglutinin. TBM numbers were determined per germinal center on adjacent sections by the presence of tingible bodies or histochemically by using the monoclonal antibody Mac-2. Analysis of lymph nodes from young mice showed that TBM numbers decreased with the dissociation of preexisting germinal centers. TBM reappeared 5 days after challenge and the TBM kinetics paralleled the increase in size of de novo germinal centers. In fact, a constant ratio of one TBM to every 350-450 B cells was maintained from day 5 to day 10. In old lymph nodes, TBM were generally absent throughout germinal center development. The lack of TBM prior to germinal center development and their absence in aged mice are inconsistent with the concept that TBM are required for the induction of the germinal center reaction. However, the data are consistent with a role for TBM in regulating the magnitude of the germinal center reaction.
Anat Rec 1991 Apr
PMID:Kinetics of the tingible body macrophage response in mouse germinal center development and its depression with age. 204 55

Samples of faeces and blood were obtained from 66 adult horses with diarrhoea. The results of routine bacteriological, parasitological, haematological and biochemical tests were correlated with the outcome of the cases. Twenty-two (33 per cent) of the horses died or were destroyed as a consequence of the diarrhoea. A diagnosis was reached in only 23 cases (35 per cent), and in nine of them only at post mortem examination. Salmonella typhimurium was isolated from five cases. Statistical analysis revealed significant differences between the horses which survived and those which died in their packed cell volumes, white blood cell counts, neutrophil counts, serum albumin concentrations and alkaline phosphatase activities.
Vet Rec 1990 May 12
PMID:Diarrhoea in adult horses: a survey of clinical cases and an assessment of some prognostic indices. 219 Apr 8

Forty-six adult merino ewes were immunised against oestradiol-17 beta-6 carbomethyloxime:human serum albumin and 48 comparable ewes were used as controls in an experiment to study the effects of gonadotrophin releasing hormone (GnRH) on ovulatory responses after treatment with pregnant mare's serum gonadotrophin (PMSG). All the ewes were treated with progestogen sponges for 14 days and received 1500 iu PMSG on the 12th day. Twenty-four control and 24 immunised ewes received 25 micrograms GnRH 21.5 hours and 23 hours after the sponges were withdrawn. Plasma samples were collected between 17 and 50 hours after the sponges were withdrawn and assayed for luteinising hormone (LH). Immunisation reduced the proportion of ewes which ovulated and their rate of ovulation. Injection of GnRH increased the proportion of immunised ewes ovulating (P less than 0.0005) and their rate of ovulation (P less than 0.0001). More unovulated follicles were observed in immunised ewes regardless of GnRH treatment (P less than 0.0001). The rate of recovery of eggs was reduced after immunisation. Treatment with GnRH produced a surge of LH of equal magnitude in the control and immunised ewes although not as many immunised ewes ovulated.
Vet Rec 1987 Jun 20
PMID:Ovarian response to PMSG and GnRH in ewes immunised against oestradiol-17 beta. 330 12

Rabbits were intravenously primed and boosted with trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH) and human serum albumin (HSA); both antigens were injected simultaneously. The localization of anti-TNP-antibody-forming cells (AFCs) and anti-HSA-AFCs was determined in various lymphoid organs of the rabbit. In all lymphoid organs of primed rabbits anti-TNP-AFCs outnumbered anti-HSA-AFCs, with the exception of the thymus, in which neither of them was encountered. In the spleen the antibody-forming cells were mainly situated in the periphery of the periarteriolar lymphocyte sheaths (PALS) and in the coaxial sheaths of lymphoid tissue surrounding the terminal arterioles. In the lymphoepithelial organs AFCs were almost exclusively situated in the interfollicular areas, and in the lymph nodes largely in the medulla. An intravenous booster injection led to a secondary immune response (i.e., increase of AFCs) in the spleen. No visible change in the number of specific AFCs was observed in the lymphoepithelial organs. However, in the mesenteric and popliteal lymph node the number of anti-TNP-AFCs had increased tremendously.
Anat Rec 1987 Jan
PMID:Development of specific antibody-forming cells in various lymphoid organs of rabbit after intravenous antigen administration. 345 65


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