Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:Q9UIJ5 (
Rec
)
58,342
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Interleukin 4
(
IL-4
) plays a central role in immune responses to parasites and allergens.
IL-4
drives the differentiation of naive T cells into Th2 cells and regulates immunoglobulin class switching to IgE.Little is known about the role of
IL-4
in canine allergies and parasite infections. Most of the information derives from measurement of IL-4 mRNA expression in dog tissues, but detection of
IL-4
protein has been difficult so far, probably due to low sensitivity of available methods. Antibodies (Ab) specific for canine
IL-4
are available from various sources, but these Ab have been produced against recombinant Escherichia coli-expressed canine
IL-4
and there is only limited information on their reactivities with native canine
IL-4
. Therefore, in the present study, we tested six available canine
IL-4
-specific Ab for their reactivities with recombinant canine
IL-4
expressed in E. coli (
rec
.
IL-4
) or in mammalian cells (mam.
IL-4
), and with supernatants from stimulated canine peripheral blood mononuclear cells (PBMCs) using several detection methods, including Western blotting, ELISA, cytokine bead assay, and intracellular
IL-4
staining. Additionally, we tested a bovine
IL-4
-specific antibody that has been previously shown to cross-react with canine
IL-4
. All tested Ab except anti-bovine
IL-4
reacted with
rec
.
IL-4
, and most of them reacted with mam.
IL-4
. However, only the cytokine bead assay was sensitive enough to allow the detection of
IL-4
in supernatants of canine PBMCs.
...
PMID:Distinct reactivities of interleukin-4-specific antibodies with recombinant and native canine interleukin-4 in various assays. 2059 2
