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 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mycobacterium bovis was isolated from the respiratory tracts of three cattle which registered negative to tuberculin testing; no tuberculous lesions were found and the culture of lymph nodes and other tissues proved negative. One animal was from a group of five calves which had been inoculated intranasally with M bovis, and the organism was recovered once only from nasal mucus sampled 100 days after inoculation. The second animal had had contact with experimentally infected cattle which were excreting M bovis and the third was from a commercial farm. The results of ELISAS for antimycobacterial antibodies and interferon-gamma, and of lymphocyte transformation assays are presented. The animals' immune responses provided evidence that each of them had been challenged.
Vet Rec 1992 Jul 18
PMID:Isolation of Mycobacterium bovis from the respiratory tracts of skin test-negative cattle. 144 Nov 61

Recombinant murine interferon-gamma (Rec-MuIFN-gamma) was administered intramuscularly to C3H/HeNCrj mice on Days 6-15 of gestation at dosage levels of 8 X 10(5), 4 X 10(6), and 2 X 10(7) u/kg/day. Dams were killed for examination of fetuses on Day 18 of gestation. Pregnant females that received 2 X 10(7) u/kg/day of Rec-MuIFN-gamma showed uterine bleeding on Days 10-15 of gestation and could not maintain their pregnancy. These dams died on Days 13-17 or were killed in extremis on Days 10-15 for examination, and therefore no fetal data were available for this group. In the 2 X 10(7) u/kg/day group, the mean absolute weights of the lung and spleen increased and the mean absolute weight of the liver, red blood cells (RBC), hematocrit, and hemoglobin decreased significantly. Surviving dams in the 8 X 10(5) and 4 X 10(6) u/kg/day groups showed significant increases in the mean absolute weights of the lung, liver, kidneys, and spleen and a decrease in platelet count. Significant increases in the weights of the heart and ovaries and decreases in RBC, hematocrit, and hemoglobin were observed in the 4 X 10(6) u/kg/day group. Histopathological examination revealed increased extramedullary hematopoiesis in the spleen of the 4 X 10(6) and 2 X 10(7) u/kg/day groups. Fetuses showed no external, visceral, or skeletal malformations and variations caused by the administration of Rec-MuIFN-gamma in any of the treated groups.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Effects of recombinant murine interferon-gamma on pregnant mice and their fetuses. 211 76

Recombinant interferon-alpha-2C (rec. IFN alpha-2C) and recombinant interferon-gamma (IFN-gamma) was studied in 12 patients with metastasized renal cell carcinoma. 8 patients were treated with IFN-alpha-2C: 1 patient achieved a complete remission, 2 patients showed mixed responses, and 2 had stabilisation of their disease. In 3 patients progressive disease was observed. IFN-gamma was studied in 4 patients; 2 patients showed stable and 2 progressive disease. Side effects of IFN-alpha treatment included influenza-like symptoms, moderate hematological toxicity and neurological symptoms. During treatment with IFN-gamma similar side effects were observed, although fever generally was more pronounced. All symptoms ceased after dosis reduction or discontinuation of treatment.
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PMID:[Interferon (IFN) therapy (recombinant IFN-alpha-2C or recombinant IFN-gamma) in metastasized hypernephroma]. 393 90

Mycobacterium bovis was isolated from respiratory secretions and lymph nodes from 15 skin test-negative cattle which exhibited interferon-gamma responses. These field cases, identified by blood testing, constituted a significant proportion of skin test-negative cattle which had been subjected to extensive post mortem examinations. Typical tuberculous lesions were found in seven of them. The consequences of cattle with early tuberculosis infection not being detected by traditional tuberculin testing are considered.
Vet Rec 1994 Aug 06
PMID:Detection of Mycobacterium bovis infection in skin test-negative cattle with an assay for bovine interferon-gamma. 797 5

This study was conducted to determine whether young calves with maternal antibodies against bovine herpesvirus type 1 (BHV-1) but without antibodies against glycoprotein E (gE) can produce an active antibody response to gE after a BHV-1 infection. Five calves received at birth colostrum from gE-seronegative cows which had been vaccinated two or three times with an inactivated BHV-1, gE-deleted marker vaccine. After inoculation with a wild-type virulent strain of BHV-1, all the passively immunised gE-negative calves shed virus in large amounts in their nasal secretions. All the calves seroconverted to gE within two to four weeks after inoculation and then had high levels of gE antibodies for at least four months. The development of an active cell-mediated immune response was also detected by in vitro BHV-1-specific interferon-gamma assays. All the calves were latently infected, because one of them re-excreted the virus spontaneously and the other four did so after being treated with dexamethasone. The results showed that under the conditions of this work the gE-negative marker could also distinguish between passively immunised and latently infected calves.
Vet Rec 1999 Feb 13
PMID:Antibody response to glycoprotein E after bovine herpesvirus type 1 infection in passively immunised, glycoprotein E-negative calves. 1009 25

The continued use of purified protein derivative (PPD) tuberculin is considered to be the main factor which limits the specificity of diagnostic tests for bovine tuberculosis (TB). This study evaluated a whole blood interferon-gamma (IFN-gamma) assay and compared the diagnostic potential of PPD with two tuberculosis-specific antigens, ESAT-6 and MPB70. To provide estimates of sensitivity and specificity, responses were measured in 180 skin test-reacting cattle, of which 131 were confirmed as tuberculous, and in 128 cattle from TB-free herds. For the skin test reactors, there was a positive correlation between the IFN-gamma responses to PPD from Mycobacterium bovis (PPDB) and PPD from Mycobacterium avium (PPDA), indicating cross-reactivity between these complex antigens which are the basis of the skin test. In comparisons of the ESAT-6 IFN-gamma test with a PPD IFN-gamma test (using PPDB compared with PPDA), there was a decrease in sensitivity (76.3 per cent vs 89.3 per cent), but a clear increase in specificity (99.2 per cent vs 92.2 per cent). The provision of high specificity, even with lower sensitivity, offers major benefits for testing in areas with a low incidence of TB.
Vet Rec 2000 Jun 03
PMID:Assessment of defined antigens for the diagnosis of bovine tuberculosis in skin test-reactor cattle. 1088 54

Acute infection of calves, previously vaccinated with bacille Calmette-Guerin (BCG), with non-cytopathic viral diarrhoea virus (BVDV) resulted in the temporary suppression of two in vitro assays used to monitor Mycobacterium bovis infection. Lymphocyte proliferation and interferon-gamma production by whole blood cultures containing purified protein derivatives prepared from Mycobacterium avium (PPD-A) and M bovis (PPD-B) were markedly suppressed. The implication is that acute infections of cattle with non-cytopathic BVDV may temporarily compromise diagnostic tests for M. bovis infections and result in a failure to identify cattle with tuberculosis.
Vet Rec 2001 Oct 20
PMID:Masking of two in vitro immunological assays for Mycobacterium bovis (BCG) in calves acutely infected with non-cytopathic bovine viral diarrhoea virus. 1170 Sep 26

The pattern of expression of cytokine mRNA in the lesions of anal furunculosis was evaluated in tissue biopsies from 15 dogs, and compared with the pattern in control skin samples from 24 dogs, by reverse-transcriptase PCR using canine cytokine-specific primers and a semi-quantitative multiplex PCR assay. Interleukin-2 (IL-2) was detected in 11 of the 15 affected dogs but in only one of the controls, and interferon-gamma was detected in 14 of the affected dogs but none of the controls. In contrast, IL-4 was detected only in one of the affected dogs. Increased expression of mRNA for IL-1beta, IL-6, tumour necrosis factor alpha, IL-8, IL-10 and transforming growth factor beta1 was detected in the biopsies from the lesions of anal furunculosis relative to the control tissues (P < 0.05).
Vet Rec 2003 Sep 20
PMID:Expression of cytokine mRNA in canine anal furunculosis lesions. 1453 66

More accurate tests are required to test cattle which have reacted positively in the tuberculin skin test. For this purpose, a range of mycobacterial antigens, MPB59, MPB64, MPB70, MPB83, ESAT-6 and CFP10, were used either as recombinant proteins or as synthetic peptides in the whole blood interferon-gamma (IFN-gamma) test. Groups of uninfected cattle with typical 'non-specificity' problems were targeted, in particular animals with skin tuberculosis, animals vaccinated against Johne's disease and animals that were positive in the standard purified protein derivative (PPD)-based IFN-gamma test. The two study groups consisted of 74 Mycobacterium bovis-culture positive animals and 72 uninfected animals, all of which tested positive in the caudal fold tuberculin skin test eight to 28 days before the blood test. The use of combinations of ESAT-6 and CFP10 antigens, either as recombinant proteins or peptides, detected similar percentages of M bovis-infected animals as the PPD-based IFN-gamma test, but produced significantly fewer false positive reactions. The PPD-based IFN-gamma test was very effective in differentiating animals vaccinated against Johne's disease that were skin-test positive from those with bovine tuberculosis, and the use of PPD or specific mycobacterial antigens minimised the number of false positive reactions in animals with skin tuberculosis.
Vet Rec 2003 Nov 15
PMID:Use of mycobacterial peptides and recombinant proteins for the diagnosis of bovine tuberculosis in skin test-positive cattle. 1465 40

Since November 2002, the State Veterinary Service and the Veterinary Laboratories Agency have been conducting a field trial to assess the interferon-gamma test as an ancillary parallel test to the tuberculin skin test in herds with confirmed tuberculosis breakdowns. Here, Martin Vordermeier, Tony Goodchild, Richard Clifton-Hadley and Ricardo de la Rua describe the background to the trial, discuss the principle of the test and provide an update of progress so far
Vet Rec 2004 Jul 10
PMID:The interferon-gamma field trial: background, principles and progress. 1528 81


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