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The changes in the titres of anti-leishmania antibodies in 25 dogs with leishmaniasis were studied by using a Dot-ELISA technique while they were being treated with N-methylglucamine and allopurinol. When the diagnosis of leishmaniasis was established, 21 of the dogs had positive titres (1:800 or higher), and after treatment, 15 of them still had high titres, in most cases the same as at the point of the diagnosis. In four dogs the titres decreased, and were less than the cut-off value in two cases nine months after treatment. Only four dogs had titres less than the cut-off value when they were diagnosed, but one month later the titres in all four dogs had reached the cut-off value. There was no correlation between the serological titres and the severity of the clinical signs. It is concluded that the Dot-ELISA is a sensitive method for the diagnosis of canine leishmaniasis but is not satisfactory for monitoring the clinical development of the disease.
Vet Rec 1995 May 20
PMID:Serological diagnosis and treatment of canine leishmaniasis. 766 May 49

A study was carried out in dogs to define the pharmacokinetic profile of antimony and to define a better therapeutic protocol for the treatment of canine leishmaniasis. Six healthy beagle dogs received 100 mg/kg of N-methylglucamine antimoniate containing 27.2 per cent of antimony intravenously, intramuscularly and subcutaneously. After intravenous administration the plasma concentration of antimony decreased rapidly and after 240 minutes it was lower than the ED50 values suggested for Leishmania donovani. The pharmacokinetic parameters and bioavailability of antimony were calculated after each route of administration in each dog. The curves of plasma concentrations vs time were best described by a triexponential open model with a mean (sd) half life t1/2 alpha of 9.4 (4.4) min, a t1/2 beta of 45.3 (4.5) min and a t1/2 gamma of 618.0 (93.5) min. The mean volume of distribution at steady state was 0.25 (0.03) litres/kg and the total body clearance was 0.25 (0.04) litres/h/kg. The peak plasma concentration (Cmax) after intramuscular administration was 27.2 (3.1) micrograms/ml, and after subcutaneous administration it was 25.5 (4.5) micrograms/ml; they were reached after 73.6 (11.9) min and 85.6 (11.3) min, respectively. The bioavailabilities after intramuscular and subcutaneous administration were 91.7 (7.1) and 92.2 (7.1) per cent, respectively. More than 80 per cent of the antimony was excreted in the urine in the first nine hours.
Vet Rec 1996 Feb 24
PMID:Disposition of antimony after the administration of N-methylglucamine antimoniate to dogs. 867 19

Urinary enzyme activities of alanine aminopeptidase, gamma-glutamyl transpeptidase, alkaline phosphatase, N-acetyl-beta-D-glucosaminidase and beta-glucuronidase were determined in 15 dogs with leishmaniasis and in a group of eight normal dogs. Serum creatinine and blood urea nitrogen concentrations were also measured and renal histology was examined. All the affected dogs had renal lesions. However, no significant differences in blood urea nitrogen and creatinine concentrations were found between the control group and the affected group. The urinary enzyme activities of gamma-glutamyl transpeptidase (P < 0.01), N-acetyl-beta-D-glucosaminidase (P < 0.01) and beta-glucuronidase (P < 0.05) were significantly higher in the affected dogs. Urinary enzymes therefore seem to be a more sensitive and reliable test for assessing early renal damage in canine leishmaniasis than serum creatinine or blood urea nitrogen concentrations.
Vet Rec 1997 May 03
PMID:Enzymuria as an index of renal damage in canine leishmaniasis. 916 May 31

A slide ELISA for canine leishmaniasis was developed by using promastigotes of Leishmania infantum, and compared with microimmunodiffusion, immunoelectrophoresis, direct agglutination and indirect immunofluorescence assays. The sensitivity of all the tests was 100 per cent. The specificity of the direct agglutination test was 95 per cent but it was 100 per cent for the three other tests. There was also a positive correlation and a high level of concordance between the titres measured by the different tests.
Vet Rec 1997 Sep 27
PMID:Development of a slide ELISA for canine leishmaniasis and comparison with four serological tests. 934 21

The clinical and laboratory findings observed in 150 dogs naturally infected by Leishmania infantum, from a large endemic area of southern Italy, are described. There was a gradual onset of clinical signs and the course of the disease was progressive in almost all the cases. The majority of the dogs were mongrels (43.3 per cent), male (64.7 per cent), of medium size (50.6 per cent), three to seven years old (64.7 per cent), and living outdoors (60 per cent). They showed generalised (56.7 per cent) or symmetrical (32 per cent) lymphadenomegaly; the mucous membranes of 87 of the dogs (58 per cent) were pale and moderate or severe splenomegaly was diagnosed in 80 dogs (53.3 per cent); weight loss was observed in 32 per cent of the animals. Skin abnormalities were very common, and included dry exfoliative dermatitis (56 per cent), ulcers (40 per cent) periorbital alopecia ('lunettes') (18 per cent), diffuse alopecia (14 per cent) and onychogryphosis (24 per cent). Ocular signs were observed in 24 dogs (16 per cent) including 16 cases of keratoconjunctivitis (three with keratoconjunctivitis sicca), six cases of moderate uveitis and two cases of panophthalmitis. The acute form of the disease was diagnosed in only six dogs and was characterised by fever and generalised lymphadenomegaly, and by the absence of skin lesions. Another six dogs had severe renal failure without systemic clinical signs of leishmaniasis. The most important laboratory findings were a severe or moderate increase in gammaglobulins, hypoalbuminaemia, hyperproteinemia and anaemia. Cultures or cytology tests for L infantum parasites were positive in 134 of the dogs. Following the standard procedures developed for human lymph node and bone marrow cytology tests, the leishmania density in the dogs varied from 1+ to 2+. Leishmania antibody titres were high (> 1:160) in almost all the dogs. Immunological tests for autoantibodies were positive in 25 of 53 dogs tested in the antinuclear antibody (ANA) test, in 15 of 43 dogs tested in the latex test and in five of 24 dogs tested in the Coombs test.
Vet Rec 1997 Nov 22
PMID:A retrospective clinical study of canine leishmaniasis in 150 dogs naturally infected by Leishmania infantum. 941 21

Primary haemostasis was evaluated by measurements of bleeding time and platelet count in 26 dogs with leishmaniasis and 10 normal dogs. Bleeding time was significantly (P = 0.02) longer in the infected dogs than in the control group, and in infected dogs with creatinine concentrations > 1.5 mg/dl than in infected dogs with normal creatinine levels. There was a significant linear regression between the concentration of creatinine and bleeding time (P = 0.02) although the coefficient of determination was low (r2 = 0.194). There were no significant differences in platelet counts between the normal and diseased dogs, and there were no significant differences between male and female dogs in either group.
Vet Rec 1998 Jan 24
PMID:Evaluation of primary haemostasis in canine leishmaniasis. 949 27

Secondary haemostasis was evaluated in 26 dogs with leishmaniasis and 10 normal dogs by measurements of modified one-stage prothrombin time (m-OSPT), activated partial thromboplastin time (APTT), thrombin time, fibrinogen concentration and fibrin degradation products. There were no significant differences between the groups in the m-OSPT, fibrinogen concentration, or levels of fibrin degradation products. The APTT was significantly (P = 0.006) longer in the infected dogs than in the control group, and in infected dogs with alanine aminotransferase (ALT) activities > 50 U/litre. There was a significant linear regression between ALT and APTT. Thrombin time was significantly (P = 0.003) longer in the infected dogs than in the normal dogs. There were no significant differences between the thrombin times of sick dogs with different levels of creatinine or activities of ALT, or between male and female dogs, whether diseased or normal.
Vet Rec 1999 Feb 13
PMID:Evaluation of secondary haemostasis in canine leishmaniasis. 1009 24

A polymerase chain reaction (PCR) technique for the diagnosis of canine leishmaniasis on bone marrow samples was developed which amplified a 120 bp DNA fragment of the Leishmania kinetoplast DNA, common to all Leishmania species. Forty-five of 46 dogs in which leishmaniasis had been diagnosed were positive with the PCR technique, whereas none of 41 healthy dogs gave a positive result. Fifteen dogs with leishmaniasis that had been treated for six months with N-methylglucamine antimoniate and allopurinol were also investigated. Seven were positive, implying that they remained infected despite the resolution of their clinical signs.
Vet Rec 1999 Mar 06
PMID:Diagnosis of canine leishmaniasis by a polymerase chain reaction technique. 1042 21

Twenty-four dogs with a parasitologically and serologically established diagnosis of leishmaniasis were studied to investigate the atrophy of the masticatory muscles which commonly occurs in this disease, and to compare the lesions in the masticatory muscles with those in the cranial tibial muscles. The 24 animals were divided into three groups of eight, group A dogs with no muscular atrophy, group B dogs with different degrees of atrophy in the masticatory and skeletal muscles, and group C dogs with similar degrees of atrophy in the masticatory and skeletal muscles. Increased activities of creatine phosphokinase and lactate dehydrogenase were recorded in only some of the dogs in groups B and C, but there were no significant differences between the mean activities in the three groups. Electromyographic changes indicating myopathy and involving both the temporalis and cranial tibial muscles, were observed in two of the dogs in group A, seven of those in group B, and in all the dogs in group C. Muscle histopathology revealed a variable degree of muscle fibre necrosis and atrophy, mononuclear infiltrates and neutrophilic vasculitis in all the dogs except two in group A. Leishmanial amastigotes were found within macrophages and myofibres in 16 of the dogs, some in each group. IgG immune complexes were detected in muscle samples, and circulating antibodies against myofibres were detected in serum samples from all the 24 dogs.
Vet Rec 2000 Jun 10
PMID:Masticatory and skeletal muscle myositis in canine leishmaniasis (Leishmania infantum). 1088 83

Haemostasis was evaluated in 19 dogs with natural Leishmania infection, six of them with a history of epistaxis, and the results were compared with the results from 24 healthy dogs. In addition, the dogs' blood pressure was measured and biopsies were taken from the nasal mucosa. Buccal mucosa bleeding time was prolonged in the dogs with leishmaniasis (P < 0.002) and most significantly in those with epistaxis (P < 0.005). None of the Leishmania-infected dogs had thrombocytopenia, low levels of plasma von Willebrand factor antigen, a prolonged prothrombin time or activated partial thromboplastin time, a low plasma fibrinogen concentration or high serum fibrin degradation products. These results rule out defects of secondary haemostasis or disseminated intravascular coagulation as significant causes of epistaxis in non-complicated leishmaniasis. Histopathology of the nasal mucosa of 10 of the affected dogs, three of them with epistaxis, revealed ulcerative and inflammatory lesions in all of them.
Vet Rec 2001 Aug 11
PMID:Evaluation of the potential causes of epistaxis in dogs with natural visceral leishmaniasis. 1153 Sep 2


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