Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q9UIJ5 (Rec)
 58,342 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Specific pathogen free cats were infected by two feline calicivirus isolates of different plaque type, an extra-large plaque (ep) former and a minute plaque (mp) former. A comparison was made of the disease produced when these isolates were administered by either aerosol or direct intranasal instillation. With both isolates, aerosol infection produced lesions and gave rise to virus replication throughout the respiratory tract. The effects of intranasal infection were more confined to the upper respiratory tract and oropharynx. By both routes of infection the disease produced by the mp virus was clinically and pathologically less severe than that produced by the ep virus.
Vet Rec 1979 Jan 27
PMID:Diseases produced by feline caliciviruses when administered to cats by aerosol or intranasal instillation. 43 13

"Uncouplers" of oxidative phosphorylation (sodium azide, DNP, and oligomycin) alter the location of microtubules within murine mast cells. Both cytoplasmic microtubules, perpendicular to the plasma membrane within cell surface folds, and intranuclear microtubules were observed. In addition, one or more dense plaque-like structures adjacent the plasma membrane in mast cells appeared following incubation in the antimetabolities. Intranuclear microtubules and cytoplasmic microtubules within the cell surface ridges disappeared in azide-treated mast cells that were reincubated or "recovered" in PBS. However, both these structures remained in oligomycin- and DNP-treated murine mast cells following reincubation.
Anat Rec 1979 Oct
PMID:Effect of uncouplers of oxidative phosphorylation on microtubule location and surface structure in murine mast cells. 50 98

The thyroid gland of the C3H mouse is composed largely of the usual follicle but it also contains a second kind of follicle. To ascertain the embryologic origin of the cell types in each of these follicles, ventral pharyngeal outpocketings and ultimobranchial outpocketings were isolated (before they fused to form the thyroid gland) from the 12-day-old fetus of the C3H mouse. The outpocketings were implanted into different kidney capsules of adult C3H mice and were allowed to grow for several months. Transplants were then excised and examined by electron microscopy. The ventral contribution formed large aggregates of follicles and was recognized as a distinct bump on the surface of the kidney. It formed only typical thyroid ultimobranchial contribution usually formed only a small number of follicles, generally of small size. It was readily located because the follicles occurred next to a white plaque of bone or cartilage at the site of implantation. The ultimobranchial contribution formed follicles containing four cell types: a ciliated cell, a cell with abundant agranular reticulum, a cell with many free ribosomes and fiber and occasional hemidesmosomes, and the C cell which was the most frequent cell type. No typical thyroid epithelium was observed in the ultimobranchial transplant. These observations suggest that the C cell in the usual follicle is derived from the ultimobranchial contribution, and that the second kind of follicle is largely an ultimobranchial contribution but the typical thyroid epithelium in it is largely or entirely a ventral contribution.
Anat Rec 1978 May
PMID:Embryologic origin of the various epithelial cell types in the second kind of thyroid follicle in the C3H mouse. 64 34

A heretofore undescribed structural variation of the desmosomes of the intercalated disc is found in myocardial cells of the embryonic guinea pig. These desmosomes consist of the usual pair of opaque leaflets, each of the pair contributed by one of the apposed muscle cells. In addition, in the cytoplasm of one of the cells there appears a pair of linear densities (facsimile-lines) parallel to the nearest desmosomal plaque and separated from it by a 60 mm space. The facsimile lines superficially resemble the desmosomal leaflets in length and thickness, thus forming a cytoplasmic "image" of the desmosome. These "imaged-desmosomes" are found predominantly in the longitudinally-running portions of the intercalated discs and are common in 7-week embryos. Their incidence drops sharply by eight weeks of gestation, and they are virtually absent from the heart of the newborn animal. Often tubules of the sarcoplasmic reticulum (SR) are found in apposition to the facsimile-lines; thus it appears that association of SR tubules with desmosomes is responsible for the formation of imaged-desmosomes.
Anat Rec 1975 Oct
PMID:The "imaged-desmosome": a component of intercalated discs in embryonic guinea pig myocardium. 120 Apr 1

The surface distribution of membrane-associated dense plaques in intact quail gizzard smooth muscle cells was investigated by freeze-fracture. Replicas of fractured smooth muscle cell plasma membrane showed caveola-free regions with few intramembrane particles, interspersed with caveola-populated areas with a higher intramembrane particle density. Electron microscopy of thin sections of quail gizzard smooth muscle revealed the regions free of caveolae to be occupied by membrane-associated dense plaques; anchoring sites for the contractile filaments of the cell. Demarcation between the caveola-populated and caveola-free regions on the relicated intramembrane surface was not clear and thus provided little information concerning the distribution of dense plaque sites. However, treatment of the smooth muscle tissue with the cholesterol-binding agents filipin or tomatin prior to freeze-fracture allowed the dense plaque sites to be easily observed as the sites remained free of the membrane deformations characteristic of these agents. The dense plaque sites consist of caveola-free oval areas juxtaposed in regular bands that traverse the long axis of the cell. The dense plaque sites on the freeze-fracture replica were confirmed by electron microscopy of thin sections of filipin-treated quail gizzard smooth muscle cells, which showed the plasma membrane associated with the dense plaques to be unaffected by the actions of filipin, whereas that of the caveola-populated region was severely deformed. The observations presented in this study provide evidence for a highly ordered distribution of dense plaques at the cell surface of intact quail gizzard smooth muscle cells and thus corroborate existing evidence for an organized substructure of smooth muscle cells.
Anat Rec 1992 Mar
PMID:Ordered distribution of membrane-associated dense plaques in intact quail gizzard smooth muscle cells revealed by freeze-fracture following treatment with cholesterol probes. 154 63

The tarsometatarsal skin from 13-day-old chick embryos was treated with EDTA and/or Dispase to separate it into epidermis and dermis, and the basal lamina was removed. The isolated epidermis and dermis were then recombined and cultured on Millipore filters in a chemically defined medium (BGJb). Beginning at 3-4 days after recombination, short fragments of new basal lamina and subbasal dense plaque were formed along the epidermal basal cell outer surface immediately subjacent to hemidesmosomes. After 6-8 days of culture, fragments of the basal lamina started to fuse together and the lamina became progressively continuous. At the same time, anchoring fibrils were formed to attach to the basal lamina. The hemidesmosome formation preceded the basement membrane formation. When normal embryonic epidermis was recombined with retinol-pretreated dermis and cultured for 7 days in BGJb, short fragments of the basal lamina, the subbasal dense plaque, and anchoring fibrils were formed, but the basement membrane remained discontinuous with many interruptions in the interspace between hemidesmosomes. These results demonstrate that pretreatment of dermis with retinol causes the changes noted in the basement membrane.
Anat Rec 1991 Nov
PMID:Reconstruction of basement membrane in recombinants of epidermis and dermis of chick embryonic skin in vitro: an electron microscopic study. 176 19

The safety of an Aujeszky's disease virus vaccine based on strain 783, a deletion mutant which does not express glycoprotein I and thymidine kinase, was assessed in pigs, calves and sheep. Four-day-old piglets which were inoculated intranasally and intramuscularly with 10(7) plaque forming units (PFU) developed only slight depression and fever. The virus was transmitted to a sentinel piglet. Six weeks after inoculation, the pigs were injected with high doses of corticosteroids in an attempt to reactivate the vaccine virus. The pigs did not shed Aujeszky's disease virus, did not develop a rise in virus neutralising antibody titres and sentinel pigs remained seronegative to Aujeszky's disease virus. Strain 783 was passaged in two series of three- to five-day old piglets, but after the third and fourth passages virus could no longer be recovered. Pregnant sows were inoculated with 10(7) PFU of virus strain 783 around day 35 or on day 85 of pregnancy, and their fetuses and piglets were assayed for Aujeszky's disease virus and antibodies against Aujeszky's disease virus. No evidence was found for transplacental transmission of the virus. Calves and sheep were given 10(7) PFU of virus strain 783 intranasally or intramuscularly; they survived and did not develop clinical signs of Aujeszky's disease. All the sheep and the calves inoculated intramuscularly developed neutralising antibodies to Aujeszky's disease virus.
Vet Rec 1990 Nov 03
PMID:Safety of an Aujeszky's disease vaccine based on deletion mutant strain 783 which does not express thymidine kinase and glycoprotein I. 217 88

The formation of dental calculus in dogs is a major problem. Scanning electron microscopy of tartar specimens from dogs revealed on the outer surface of the plaque polymorphic configurations, more or less arranged as free filaments, corn-cobs or swab-like structures. Uninhabited bacterial recesses were found on the inner surface of the calculus. Calcification may occur between or within the bacteria. Elucidating the mechanisms of calculus formation should help in the development of prophylactic measures.
Vet Rec 1990 Nov 10
PMID:Use of scanning electron microscopy to investigate dental calculus in dogs. 227 95

It has recently been shown that the antibody response to glycoprotein I (gI) of Aujeszky's disease virus can be used to distinguish infected from vaccinated pigs. To examine whether pigs exposed to low doses of a mildly virulent strain of Aujeszky's disease virus produce antibody to gI four groups of four pigs were inoculated intranasally with 10, 10(2), 10(3) or 10(4) plaque forming units (PFU) of the Sterksel strain. Two unvaccinated pigs and two pigs vaccinated intranasally with Bartha's K strain, a gI-negative vaccine, were placed in contact with each group. The pigs given 10 PFU and the in-contact pigs in this group did not become infected. The inoculated and the unvaccinated in-contact pigs in the other groups developed mild signs of illness and produced antibody to gI. Four of six vaccinated in-contact pigs that became infected showed neither clinical signs nor virus shedding and still produced antibody to gI. The other two vaccinated pigs appeared to be resistant to contact-challenge. The antibody response to gI persisted for at least seven months. These results support the idea that Aujeszky's disease virus may be eradicated by a programme based on vaccination with gI-negative vaccines, in conjunction with the detection and subsequent removal of gI-antibody positive, infected, pigs.
Vet Rec 1988 Jun 18
PMID:Induction of antibodies to glycoprotein I in pigs exposed to different doses of a mildly virulent strain of Aujeszky's disease virus. 284 89

As a prerequisite to mutational analysis of functional sites on the RecA protein of Escherichia coli, a method was developed for rapid isolation of recA mutants with altered RecA protease function. The method involves plating mutagenized lambda recA+ cI ind on strains deleted for recA and containing, as indicators of RecA protease activity, Mu d(Ap lac) fusions in RecA-inducible genes. The lambda recA phages were recognized by their altered plaque colors, and the RecA protease activity of the lambda recA mutant lysogens was measured by expression of beta-galactosidase from dinD::lac. One class of recA mutants had constitutive protease activity and was designated Prtc; in these cells the RecA protein was always in the protease form without the usual need for DNA damage to activate it. Some Prtc mutants were recombinase negative and were designated Prtc Rec-. Another class of 65 recA mutants isolated as being protease defective were all also recombinase defective. Unlike the original temperature-dependent Prtc Rec+ mutant (recA441), the new Prtc Rec+ mutants showed constitutive protease activity at any growth temperature, with some having considerably greater activity than the recA441 strain. Study of these strong Prtc Rec+ mutants revealed a new SOS phenomenon, increased permeability to drugs. Use of this new SOS phenomenon as an index of protease strength clearly distinguished 5 Prtc mutants as the strongest among 150. These five strongest Prtc mutants showed the greatest increase in spontaneous mutation frequency and were not inhibited by cytidine plus guanosine, which inhibited the constitutive protease activity of the recA441 strain and of all the other new Prtc mutants. Strong Prtc Rec+ mutants were more UV resistant than recA+ strains and showed indications of having RecA proteins whose specific activity of recombinase function was higher than that of wild-type RecA. A Prt+ Rec- mutant with an anomalous response to effectors is described.
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PMID:Plaque color method for rapid isolation of novel recA mutants of Escherichia coli K-12: new classes of protease-constitutive recA mutants. 316 Jun 86


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