Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q96DG6 (Pseudomonas)
 76,258 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A toxic substance, which destroyed leucocytes from man but was inactive against erythrocytes, was demonstrated in cultures of four out of 110 strains of Pseudomonas aeruginosa tested. The toxin, designated 'leucocidin', was cell-bound as a precursor toxin, exhibiting little or no toxicity. It was converted into toxin with maximum activity by various proteases including an endogenous elastase. The production of leucocidin was directly proportional to the number of bacteria and was not influenced by variations in media, iron concentration, pH or temperature. The best method for large-scale production of leucocidin was autolysis of washed bacteria.
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PMID:Formation and isolation of leucocidin from Pseudomonas aeruginosa. 0 21

Using 20 strains of Pseudomonas aeruginosa isolated from patients, production of protease, elastase, and collagenase was determined by shaking culture in either complex or semisynthetic medium. No collagenase was produced by any strain of P. aeruginosa. According to their production of protease and elastase in different media, the P. aeruginosa strains were divided into three groups: the first group can produce elastase in complex medium and both protease and elastase in semisynthetic medium; the second group cannot produce any proteolytic enzymes in complex medium but can produce any proteolytic enzymes in either medium; and the third group cannot produce any proteolytic enzymes in either medium. In spite of the differences in the ability of the strains to produce the enzymes, depending upon the origin of the strains, the protease or elastases produced in different broths were regarded as identical.
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PMID:Production of protease and elastase by Pseudomonas aeruginosa strains isolated from patients. 1 45

Plasma samples from healthy adults were examined for antibodies to the common protective antigen (OEP) [1] by passive hemagglutination (HA) reaction [2] with the finding that the majority of them had an antibody titer of 60 or lower. 2-Mercaptoethanol (2-ME) treatment, however, caused a decrease in HA titer down to 16 or lower in most of the cases, suggesting that the OEP antibody resides mostly in IgM and slightly in IgG. The finding was corroborated by gel filtration on Sephadex G-200. It appears likely that IgA is not implicated in the HA titer since IgA was HA negative. However, the detection of enzymatic activity by enzyme-linked immunosorbent assay (ELISA) [9] still suggests a possibility that OEP antibody resides in IgA. On the other hand, antibodies to proteolytic enzymes, especially protease and elastase [3], elaborated by Pseudomonas aeruginosa, were found to be present in most cases at a titer of 16 or lower by HA reaction. It was also found that they reside mostly in IgM.
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PMID:Pseudomonas aeruginosa antibodies in human plasma. 6 May 2

The effects on mortality of supplemental injections of protease and elastase were determined in burned mice infected with non-lethal inocula of a toxin-producing but non-proteolytic-enzyme-producing strain of Pseudomonas aeruginosa. When a variety of solutions containing proteolytic enzyme were injected under these conditions, the mortality increased significantly. This did not occur when organisms other than P. aeruginosa were used. Injections of the enzyme solutions alone were non-lethal. Injection of a solution of alpha 2-macroglobulin, which was shown to inhibit proteolytic activity, together with a proteolytic enzyme--toxin producing strain of P. aeruginosa caused a significant delay in mortality when compared with controls. It was concluded that protease, elastase, and toxin production were necessary for P. aeruginosa to express full virulence in the burned mouse model.
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PMID:Experimental studies of the pathogenesis of infections due to Pseudomonas aeruginosa: extracellular protease and elastase as in vivo virulence factors. 8 91

The effectiveness of immunizing mice with protease toxoid (PT) or elastase toxoid (ET) on the corneal ulcerization due to either protease or elastase were investigated. Consequently, mice immunized with either PT or ET were protected from corneal ulcers experimentally induced by the homologous enzyme, either protease or elastase. Similarly, two kinds of rabbit immune sera, anti-PT and anti-ET, were found to prevent corneal ulcers by the homologous enzyme. Then, the therapeutic effects of vaccination with a single or mixed vaccine consisting of one, two or three components, i.e., PT, ET and/or the common antigen (OEP) of Pseudomonas aeruginosa, were examined on corneal ulcers in mice produced by live cultures of the bacteria. For the same purpose, administration of a single or combined rabbit immune sera against PT, ET and OEP was conducted. As a result, vaccination with the three-component-mixed vaccine or administration with rabbit immune sera combined with anti-PT and anti-ET sera in addition to anti-OEP serum, were found to be the most effective in preventing corneal ulceration as well as in treating corneal ulcers in mice.
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PMID:Therapeutic effect of immunization with OEP, protease toxoid and elastase toxoid on corneal ulcers in mice due to Pseudomonas aeruginosa infection. 9 24

Toxoids of protease and elastase of Pseudomonas aeruginosa were successfully prepared by treatment with 8% formalin plus 0.2 m lysine and by 4% formalin respectively. The two toxoids proved sufficiently potent to elicit high antibody titers as estimated by both the enzyme-neutralizing and passive hemagglutination tests. The effectiveness of immunizing minks with a single component-vaccine consisting of the common antigen (OEP) of P. aeruginosa or the protease toxoid (PT) or the elastase toxoid (ET) and with the two (PT and ET) or the three (OEP, PT and ET) component-mixed vaccines, on hemorrhagic pneumonia in minks due to the bacteria was investigated. Female Sapphire minks, 3.5 months old, were used in two experiments performed in 1975 and 1976. Minks were immunized three times in one month with a total of 1 mg of each of the three antigens in the case of a single component vaccine and with a total of 2 or 3 mg (equal amounts of each component) in the case of the two or three component vaccines. Two or three weeks after the last immunization, challenge exposure with strain No. 5 was carried out by intranasally inoculating an inoculum containing serial dilutions of 10(3) -10(10) of live bacteria. Summarizing the results of the two experiments, in the case of controls, nonimmunized minks and minks immunized with potassium aluminum sulfate (potash alum) alone, the LD50 values were approximately 10(3) -10(4) with no significant difference between the two. In the case of OEP-vaccinated minks, the LD50 value was about 10(6) and thus clearly differed from those of the controls. In minks immunized with the three-component-vaccine, however, the LD50 value was about 10(8) -10(9), which indicated that the three-component-mixed vaccine was remarkably more effective than the single OEP vaccine component. In minks immunized with either PT or ET or both, the LD50 values were about 10(8) -10(9). The effectiveness of the vaccine made with ET or PT alone is discussed in the text. The pathological findings of the minks which died or survived are described.
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PMID:Effectiveness of immunization with single and multi-component vaccines prepared from a common antigen (OEP), protease and elastase toxoids of Pseudomonas aeruginosa on protection against hemorrhagic pneumonia in mink due to P. aeruginosa. 10 98

Strains of Pseudomonas aeruginosa were studied from 100 patients with bacteremia. In vitro quantitation of extracellular enzymes (lecithinase, protease, and elastase) and pyocine typing of these isolates were performed. No significant difference was found in the quantity of the enzymes produced or in the pyocine types by isolates obtained from patients dying from bacteremia or surviving this serious infection. Quantitation of the extracellular enzymes and pyocine types of blood isolates were contrasted with similar data obtained from sputum, urine, and skin isolates. One third of all strains produced minimal or no extracellular enzymes regardless of their source. However, the highest enzyme-producing strains were observed in the blood isolates. Although a greater variability of pyocine types was found in blood culture isolates, there was no significant difference between the pyocine types found in blood, urine, sputum, or skin isolates. The lack of correlation between the in vitro quantity of lecithinase, protease, and elastase produced by P. aeruginosa strains isolated from bacteremic patients and the prognosis of these patients supports the possible local rather than systemic significance of these extracellular enzymes in the pathogenesis of P. aeruginosa bacteremia.
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PMID:Pseudomonas aeruginosa bacteremia: relationship of bacterial enzyme production and pyocine types with clinical prognosis in 100 patients. 10 56

The effect of the iron content of the medium on the yields of extracellular products by seven distinct strains of Pseudomonas aeruginosa was examined. All strains showed at least an 85% decrease in toxin A yields when grown in medium containing 5.0 mug of iron per ml (high iron) as compared to 0.05 mug/ml (low iron), whereas bacterial growth increased approximately twofold. During the course of examining extracellular products produced by P. aeruginosa, we found many strains that produced an extracellular factor which agglutinated erythrocytes. This hemagglutinin was nondialyzable, heat stable, and resistant to Pronase and trypsin. The effect of iron on extracellular yields of hemagglutinin was strain dependent; four of seven strains showed decreases in hemagglutinin yields in high-iron medium. Similarly, the effect of increasing the iron concentration of the growth medium on yields of total extracellular proteases or on elastase was strain dependent. The amount of total extracellular protein was decreased by at least 31% in the high-iron medium for all strains of P. aeruginosa examined. Detailed studies on one strain (WR-9) showed that, in the presence of increasing amounts of iron in the medium, the extracellular yields of toxin A, protease, and hemagglutinin were decreased in a similar manner. In addition, the kinetics of release of these extracellular products were similar at a given iron concentration. Thus it appears that the yields of other extracellular products of P. aeruginosa besides toxin A are influenced by the concentration of iron in the growth medium.
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PMID:Influence of iron on yields of extracellular products in Pseudomonas aeruginosa cultures. 10 50

Passive hemagglutination (PHA) tests using fixed sheep erythrocytes coated with exotoxin of Pseudomonas aeruginosa were devised for estimating antibodies of the exotoxin. No serological cross-reactivity among exotoxin OEP, protease and elastase of P. aeruginosa was foud by the PHA tests. The exotoxin PHA reaction was more sensitive than the neutralizing reaction of skin necrotizing activity of exotoxin by guinea pigs. Thirteen out of 40 sera of patients with P. aeruginosa infections showed PHA titers from 40 to 1,280, while those of 20 healthy human sera were under 40.
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PMID:Passive hemagglutination reaction using formalinized sheep erythrocytes treated with tannic acid and coated with exotoxin of Pseudomonas aeruginosa. 10 39

The present study indicates that crystalline elastase of Pseudomonas aeruginosa is a very potent inactivator of human plasma alpha 1-proteinase inhibitor, the enzyme (E) inactivated the inhibitor (I) almost completely within 1 h at 25 degrees C at a molar ratio of E/I = 1:100. The crystalline P. aeruginosa protease also inactivated the inhibitor, but 100-fold less. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the alpha 1-proteinase inhibitor inactivated by the elastase and protease showed decreases in molecular weight of approximately 5,000 and 10,000, respectively. Regeneration of trypsin was negligible even when bovine trypsin-alpha 1-proteinase inhibitor complex (E/I = 1.0) was treated with the elastase. The affinity of alpha 1-proteinase inhibitor to trypsin was much higher than that to elastase. It was suggested that, assuming the pseudomonal proteases are produced and can inactivate alpha 1-proteinase inhibitor in vivo during pseudomonal diseases, the loss of alpha 1-proteinase inhibitor activity may permit the endogenous serine proteases to cause tissue destruction.
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PMID:Protease and elastase of Pseudomonas aeruginosa: inactivation of human plasma alpha 1-proteinase inhibitor. 11 Jun 91


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