Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q96DG6 (Pseudomonas)
 76,258 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Antisera from rabbits immunized with the synthetic disaccharide paratose 1 leads to alpha 3 mannose, representive of Salmonella O-antigen 2, covalently linked to bovine serum albumin (BSA), were used in indirect immunofluorescence studies for the identification of Salmonella serogroup A (O-antigen 1,2,12) bacteria. Among 1311 enteric bacteria tested, 497 were Salmonella. The anti-paratose 1 leads to alpha 3 mannose-BSA serum identified correctly all the 63 serogroup A strains tested. No positive reactions were recorded among 1248 strains respresenting Salmonella other than serogroup A, E. coli, Shigella, Citrobacter, Klebsiella, Enterobacter, Serratia, Proteus, Pseudomonas, Acinetobacter, Vibrio, Yersinia and Bacteroides. The study illustrates the high specificity of the antiserum elicited by immunization with the synthetic disaccharide-protein immunogen.
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PMID:Synthetic disaccharide-protein antigen for production of specific O2 antiserum for immunofluorescence diagnosis of salmonella. 7 59

Rabbit antiserum against live or heat-killed Bacteroides fragilis NCTC 9343 bacteria was titrated against hot phenol water-extracted polysaccharide antigens from five different species of the 'B. fragilis group' of bacteria using an enzyme immunoassay and shown to be specific for the B. fragilis NCTC 9343 polysaccharide. When the antiserum was used in indirect immunofluorescence, 97.1% of 244 B. fragilis strains were correctly identified. Only 8 of the other 312 Bacteroides strains were stained by the anti-B fragilis serum. The 'cross-reactive' Bacteriodes strains all belonged to the "B. fragilis group" of bacteria (i.e., B; distasonis, B. ovatus, B. thetaiotaomicron, B. uniformis, and B. vulgatus). None of the 425 aerobic enteric bacteria representing Salmonella, E. coli, Proteus, Yersinia, Shigella, Klebsiella, Enterobacter, Citrobacter, and Pseudomonas were positive using the anti-B. fragilis serum. Likewise, all the 59 gram-positive strains representing Streptococcus, Bacillus, Peptostreptococcus, Peptococcus, Propionibacterium, Lactobacillus, Eubacterium, and Clostridium did not stain. Our data shows in accordance with other findings [11], that B. fragilis strains possess a species-specific cell envelope antigen(s) which promises to be important for production of antisera, making a rapid identification of the species possible.
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PMID:Identification of Bacteroides fragilis by indirect immunofluorescence. 9 51

The effect of Listeria monocytogenes lipids on the course of infections with Salmonella enteritidis, Yersinia pseudotuberculosis, Pseudomonas aeruginosa and Klebsiella pneumoniae was studied in mice. The lipids were extracted with chloroform and methanol and used in various doses before and after infection with the bacilli. The lipids significantly increased mouse resistance toward the examined bacteria. The protective effect depended, to a large extent on the applied dose of lipids and its timing.
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PMID:Effect of Listeria monocytogenes lipids on the course of infections with some gram-negative bacilli in mice. 10 64

A total of 69 dyes were incorporated separately at different concentrations into an agar medium for evaluation of their effects on the quantitative recovery of five serotypes of Yersinia enterocolitica. One strain of Pseudomonas aeruginosa and one strain of Bacillus cereus were included for comparative purposes. Certain dyes were evaluated further for their selective properties with five additional serotypes of Y. enterocolitica, three strains of P. aeruginosa, and two of Engerobacter spp. Metanil yellow was the only dye which was tolerated bettr by Y. enterocolitica than by P. aeruginosa. Dye sensitivity was variable amond strains of the same serotype of Y. enterocolitica. In general, Y. enterocolitica showed a tolerance to dyes greater than that of gram-positive bacteria and similar to that of other gram-negative bacteria.
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PMID:Effect of dyes on the quantitative recovery of Yersinia enterocolitica. 11 50

The synthetic disaccharides abequose 1 leads to a 3 mannose and tyvelose 1 leads to a 3 mannose, representative of Salmonella O-antigen 4 and 9 respectively, were covalently linked to bovine serum albumin (BSA) . Antisera from rabbits immunized with these immunogens were used in indirect immunofluorescence assay for the identification of group B (O-antigen 4) and D (O-antigen 9) Salmonella. A total of 1030 enteric bacterial strains were tested, including 207 group B and 55 group D Salmonella. The anti-abequose-mannose-BSA serum correctly identified all Salmonella group B strains tested. The anti-tyvelose-mannose-BSA serum correctly indentified all Salmonella group D bacteria examined with the exception of 11 of 18 Vi-positive S. typhi strains which did not not stain until the Vi-antigen was removed by boiling. Among the 768 strains representing Salmonella other than groups B and D, E. coli, Shigella, Citrobacter, Klebsiella, Enterobacter, Serratia, Proteus, Vibrio, Pseudomonas, Aeromonas, Yersinia, Bacteroides and Fusobacterium only 5 positive reactions were found. These were observed with Y. pseudotuberculosis strains which have the same disaccharide antigenic determinants as Salmonella O-antigen 4 and 9 respectively. The high specificity of the antisera elicited by the synthetic disaccharide-BSA immunogens make them suitable for a specific and rapid identification of Salmonella bacteria belonging to serogroups B and D.
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PMID:Synthetic disaccharide-protein antigens for production of specific 04 and 09 antisera for immunofluorescence diagnosis of salmonella. 32 49

The antimicrobial activity of a series of fluoro derivatives of benzothiadiazine and sulfonamides was studied. The compounds tested can be grouped as: a) 3-alkylmercapto derivatives of 6-trifluoromethyl-1,2,4-benzothiadiazine-1,1-dioxide (III leads to VI); the 3-mercapto precursor (VII) and the related 3-picolinic salt (VIII); b) 3-trifluoromethyl derivatives of 1,2,4-benzothiadiazine-1,1-dioxide and of its benzene substituted derivatives (IX leads to XVI); c) trifluoroacetylaminobenzenesulfonamides (XVII leads to XXV). Two of the 3-alkylmercapto compounds [(V) and (VI)] showed marked inhibitory activity against some strains of Staphylococcus, Streptococcus and Diplococcus. None of the compounds tested proved active against Gram-negative schizomycetes (genera Salmonella, Shigella, Escherichia, Proteus, Pseudomonas, Enterobacter, Klebsiella, Serratia, Yersinia, Providencia) or against yeasts (Candida).
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PMID:[Antimicrobial activity of derivatives of 1,2,4-benzothiadiazine-1,1-dioxide. VIII]. 52 7

A new agar medium for isolation of Yersinia enterocolitica was formulated based on growth studies which defined an optimum basal, and the evaluation of selective chemical agents, dyes, and antibiotics. The final formulation, designated cefsulodin-irgasan-novobiocin(CIN) agar, provided quantitative recovery of 40 different strains of Y. enterocolitica in 24 h using incubation at 32 degrees C or with 48 h of incubation at 22 degrees C. The medium was highly selective, especially against Pseudomonas aeruginosa. Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. Colony morphology coupled with a differential reaction resulting from mannitol fermentation permitted discrimination of Y. enterocolitica from most of those Gram-negative bacteria that were able to grow on the medium. Recovery and selective characteristics of CIN agar were stable during storage at room temperature for 9 days. CIN agar gave a higher recovery of Y. enterocolitica from feces both direct and with cold enrichment (0.4/1.5%) than Salmonella-Shigella (0.0/0.7%) and MacConkey (0.0/0.9%) agars while significantly reducing the level of background organisms.
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PMID:Synthesis of a selective agar medium for Yersinia enterocolitica. 54 Feb 56

In the period July 1976 to June 1977 a total of 1358 fecal specimens and 165 mesenteric lymphnodes of healthy slaughterhouse pigs were examined for Yersinia enterocolitica (Y.e.). The animals originated from 215 farms in 86 localities of Northern Bavaria. Y.e. was found in fecal specimens of 371 pigs (27.3%). A total of 408 strains was isolated including 35 double and one triple infections. Most cultures belonged to serogroups O:6...(186 strains), O:7...(78 strains), and O:5...(71 strains, Table 3). The serogroups O:3 and O:9 which in Europe are most frequently associated with human disease were isolated from 26 animals (1.9%). Lymphnodes were positive in two instances only (1.2%). Besides aerobic subculture on SS-agar after cold enrichment in phosphate buffered saline anaerobic incubation was performed simultaneously during the last 8 months of the study. This method rendered more than twice as many isolations due to an effective inhibition of environmental bacteria with oxidative metabolism (mainly Pseudomonas spp.; Tables 3 and 4). The incidence of asymptomatic infections was markedly related to season. The lowest incidence was observed during the summer months (August 1976:0%) but increased steadily to a maximum in April 1977 (71.2%; Table 4). With one exception the serogroups O:3 and O:9 were only isolated during October to December (Fig. 1). Despite the frequent occurrence of Y.e. in healthy pigs the significance of these animals for human yersiniosis remains to be clarified. Especially the frequency of disease in infants and young children would not suggest porc meat as an important vehicle of transmission. It is imaginable that the human pathogenic serogroups O:3 and O:9 might be simultaneously adapted to several hosts with independent cycles of infection. Future investigations will mainly have to consider the elucidation of the hitherto unknown mode of transmission of human yersiniosis.
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PMID:[Season-related incidence of Yersinia enterocolitica in fecal material of healthy slaughterhouse pigs (author's transl)]. 54 51

Series of alkyl derivatives of the following have been prepared: 5,7-dichloro- [compounds (II leads to V)], 6-methyl- [compounds (VI leads to IX)] and 6-methoxy-3-mercapto-1,2,4-benzothiadiazine-1,1-dioxide [compounds (X leads to XIII)]. The products were tested for antimicrobial activity. Studies were also made of the corresponding 3-mercapto precursors (XIV, XV, XVI) and the relative 3-picolinium salts (XVII, XVIII, XIX) and also of the 3-picolinium salts of 6-chloro-, 7-chloro- and 6,7-dichloro-3-mercapto-1,2,4-benzothiadiazine-1,1-dioxide (XX, XXI, XXII). Some of the 3-alkylmercapto compounds, and especially the 5,7-dichloro derivative, inhibited various strains of Gram-positive bacteria of the genus Staphylococcus, while the same substances proved much less effective against the genera Streptococcus and Diplococcus. Antimicrobial activity appeared to be influenced by the length of the alkyl chain as well as by the nature and position of the substituents on the benzene ring. The compounds proved inactive against the Gram-negative schizomycetes (Salmonella, Shigella, Escherichia, Proteus, Pseudomonas. Enterobacter, Klebsiella, Serratia, Yersinia, Providencia) and against yeasts (Candida) with the exception of compound (V) which showed slight bacteriostatic action against three strains of Candida albicans.
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PMID:[Antimicrobial effect of derivatives of 1,2,4-benzothiadiazin-1,1-dioxide. VII]. 55 23

Swedish children and adults (648 patients) with acute diarrhoea were investigated for enterotoxigenic strains in stool cultures. A total number 74 strains were isolated from 28 patients and assayed in the rabbit intestinal loop test and the adrenal cell test. Only three of the enterotoxigenic E. coli (ETEC) isolates belonged to classical enteropathogenic serotypes of E. coli (EPEC). Two enterotoxigenic strains of Proteus morganii, two of Enterobacter hafniae and one of Citrobacter freundii were isolated. None of 67 EPEC strains were found to produce a heat-labile enterotoxin (LT) in either of the two test systems. A number of Yersinia enterocolitica and Pseudomonas aeruginosa strains from stool cultures often produced toxic effects in the cell test but no enterotoxin activity was detected for any of the strains investigated either in the adrenal cell test for heat-labile enterotoxin (LT) or the suckling mouse test for heat-stable enterotoxin (ST). All EPEC isolates were also tested for ST and for invasive properties in the Sereny test; each isolate was negative in both test systems. It is concluded that production of LT and ST enterotoxin were common in stool isolates from Ethiopian children but a rare phenomenon among Swedish children with acute infantile diarrhoea. Isolation of aerobic stool bacteria with invasive properties seems to be uncommon both in Ethiopian and Swedish children. However, since both LT and ST as well as invasive properties seem to be very unstable genetic properties in many of these stool isolates improved sensitive methods for the last two properties will probably change this picture in the future.
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PMID:Relative importance of enterotoxigenic and invasive enteropathogenic bacteria in infantile diarrhoea. 73 53


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