Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:Q8TEW0 (
Par3
)
364
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The evolutionarily conserved cell polarity protein
Par3
, a scaffold-like PDZ-containing protein, plays a critical role in the establishment and maintenance of epithelial cell polarity. Although the role of
Par3
in establishing cell polarity in epithelial cells has been intensively explored, the function of
Par3
in hematopoietic cells remains elusive. To address this issue, we generated GST-fusion proteins of
Par3
PDZ domains. By combining the GST-pull-down approach with liquid chromatography-tandem mass spectrometry, we identified 10 potential novel binding proteins of PDZ domains of
Par3
in Jurkat cells (a T-cell line). The interaction of
Par3
with three proteins--nuclear transport protein importin-alpha4 and proteasome activators
PA28beta
and PA28gamma--was confirmed using in vitro binding assay, co-immunoprecipitation assay and immunofluorescence microscopy. Our results have the potential to uncover novel functions of the cell polarity protein
Par3
in blood cells.
...
PMID:Proteome identification of binding-partners interacting with cell polarity protein Par3 in Jurkat cells. 1868 89
