Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:Q8NEX9 (reductase)
 26,410 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The activities of cis-delta 3-trans-delta 2-enoyl-CoA isomerase (EC 5.3.3.8), 3-hydroxyacyl-CoA epimerase (EC 5.1.2.3), and 2,4-dienoyl-CoA reductase, all of which have been proposed to function as auxiliary enzymes in the beta-oxidation of polyunsaturated fatty acids, have been determined in mitochondria from rat heart and rat liver. In heart mitochondria the activity of 3-hydroxyacyl-CoA epimerase was lower, whereas that of 2,4-dineoyl-CoA reductase was higher than the observed rate of linoleate degration. This observation suggests that 2,4-dienoyl-CoA reductase and not 3-hydroxyacyl-CoA epimerase functions as an auxiliary enzyme in the metabolism of polyunsaturated fatty acids in heart. A modified pathway of linoleate degradation is presented.
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PMID:The activity of 3-hydroxyacyl-CoA epimerase is insufficient to account for the rate of linoleate oxidation in rat heart mitochondria. Evidence for a modified pathway of linoleate degradation. 669 54

The spectrophotometric assay of 2,4-dienoyl coenzyme A (CoA) reductase (EC 1.1.1.34) was modified to improve the linearity and sensitivity of this method. 5-Phenyl-2,4-pentadienoyl-CoA, which has an absorbance maximum at 340 nm with an extinction coefficient of 44,300 M-1 cm-1, was synthesized and used as substrate. This compound is reduced by nicotinamide adenine dinucleotide phosphate (NADPH)-dependent 2,4-dienoyl-CoA reductase to 5-phenyl-3-pentenoyl-CoA. When a tissue homogenate serves as an enzyme source, the product is further metabolized by delta 3, delta 2-enoyl-CoA isomerase (EC 5.3.3.8) to 5-phenyl-2-pentenoyl-CoA, which is hydrated to 5-phenyl-3-hydroxypentanoyl-CoA by enoyl-CoA hydratase (EC 4.2.1.17). The modified assay method, which measures the decrease in absorbance at 340 nm due to the reduction of 5-phenyl-2,4-pentadienoyl-CoA and the oxidation of NADPH, is linear for a longer period of time and is twice as sensitive as the conventional assay with 2,4-decadienoyl-CoA as substrate.
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PMID:Spectrophotometric assay of 2,4-dienoyl coenzyme A reductase with 5-phenyl-2,4-pentadienoyl-coenzyme A as substrate. 796 74