Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q8NB91 (FAB)
 3,573 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The reaction of Co(2)(CO)(8) with alkyne-containing amino acids [1a: phenylalanine (Phe) and 1b: methionine (Met)], two suitably alkyne-functionalized derivatives of the neuropeptide enkephalin (Enk) [3: Ac-Enk-Prop and 5: Ac-Enk(Pgl)-NH(2) (Ac--Acetyl; Pgl--propargylglycine; Prop--propargylamine)], a thymine Peptide Nucleic Acid (T-PNA) monomer (7), and a PNA-like monomer (9) derivative gave the respective dicobalthexacarbonyl bioconjugates in very good yields. Two different sites for labeling of the biomolecules were successfully used: The organometallic moiety was reacted with the C-terminus of alkyne-containing amino acids, peptide or PNA thymine monomers, and alternatively the organometallic compound was complexed to an internal site in the peptide or PNA. To this end, a simple glycine was replaced by propargylglycine in peptides, and a new alkyne-containing PNA-like monomer, in which an alkyne chain replaces the nucleobase, was used for PNA chemistry. For the synthesis of the two alkyne-containing enkephalin derivatives 3 and 5, two different resins, namely sulfamylbutyryl and Rink amid, were used as they allow to selectively insert, on the solid phase, an alkyne moiety at the C-terminus and on a side-chain of a peptide sequence, respectively. The identity and constitution of all cobalt complexes were confirmed by different analytical methods (IR, FAB, ESI-MS, and NMR). Most notably, IR spectroscopy shows intensive bands in the 2100-2000 cm(-1) region because of the Co(2)(CO)(6) moiety. In both (1)H NMR spectra of the dicobalthexacarbonyl PNA monomer derivatives 8 and 10, all signals are doubled because of the cis-trans isomerism about the central amide bond. The X-ray structure of a dicobalthexacarbonyl phenylalanine derivative (2a) confirms the proposed composition of the bioconjugates and shows that, as anticipated, the alkyne group of 2a is no longer linear upon complexation in comparison to the alkyne group of the bioconjugate precursor 1a, as indicated by a C-C[triple bond]C angle of about 143 degrees in 2a. Moreover, the C[triple bond]C bond of 1a was elongated by about 0.15 A upon Co(2) coordination.
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PMID:Synthesis and characterization of dicobalthexacarbonyl-alkyne derivatives of amino acids, peptides, and peptide nucleic acid (PNA) monomers. 1932 29

This study aimed to evaluate liver and breast muscle insulin-like growth factor I (IGF-I) and growth hormone receptor (GHR) gene expression between broilers fed different methionine levels and sources. Broiler chicks were 22 to 42 days old, distributed in 5 treatments (control diet, DL1 - 0.08% DL-methionine, DL2 - 0.24% DL-methionine, MHA-FA1 - 0.11% methionine hydroxy analogue-free acid, and MHA-FA2 - 0.33% methionine hydroxy analogue-free acid). The broilers were euthanized by cervical dislocation. RNA was extracted from liver and breast muscle, followed by cDNA synthesis and amplification using qRT-PCR. DL2 methionine supplementation provided best animal performance results. GHR and IGF-I gene expression in the muscle tissue was not affected by methionine supplementation. IGF-I gene expression in the liver was higher in animals fed methionine supplementation than in animals fed control diet. IGF-I mRNA levels in broilers fed DL2 were greater than DL1 (1.56 vs 0.97 AU) and greater than MAH-FA1 and MAH-FA2. Broilers fed DL2 increased significantly GHR gene expression in the liver than animals fed the control diet. Addition of methionine improved animal performance by stimulating synthesis and release of growth factor.
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PMID:Dietary methionine effects on IGF-I and GHR mRNA expression in broilers. 2439 Sep 90


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