Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q8IXL6 (RNS)
 1,091 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The only RNase genes that have been cloned from higher plants are those expressed in species that are known to exhibit self-incompatibility, such as the S genes of Nicotiana alata. In this report, we investigated whether the expression of this particular type of RNase gene is restricted to self-incompatible species, or if similar genes are expressed in other plants. Using a polymerase chain reaction approach we have identified a set of three putative RNase genes in the self-compatible plant Arabidopsis thaliana (L.) Heynh. ecotype Columbia. These A. thaliana genes, designated RNS1, RNS2, and RNS3, do not cross-hybridize to each other, and their products are homologous to both the S-gene RNases of N. alata and a set of related fungal enzymes. The A. thaliana RNS1, RNS2, and RNS3 genes encode transcripts of 1.1, 1.3, and 1.1 kilobases, respectively, and of the three genes, RNS2 is the most highly expressed in whole plants. The identification of the RNS genes in a self-compatible species suggests that this class of RNases is of general significance in RNA catabolism in higher plants.
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PMID:Genes with Homology to Fungal and S-Gene RNases Are Expressed in Arabidopsis thaliana. 1666 84

RNA fragments deriving from tRNAs (tRFs) exist in all branches of life and the repertoire of their biological functions regularly increases. Paradoxically, their biogenesis remains unclear. The human RNase A, Angiogenin, and the yeast RNase T2, Rny1p, generate long tRFs after cleavage in the anticodon region. The production of short tRFs after cleavage in the D or T regions is still enigmatic. Here, we show that the Arabidopsis Dicer-like proteins, DCL1-4, do not play a major role in the production of tRFs. Rather, we demonstrate that the Arabidopsis RNases T2, called RNS, are key players of both long and short tRFs biogenesis. Arabidopsis RNS show specific expression profiles. In particular, RNS1 and RNS3 are mainly found in the outer tissues of senescing seeds where they are the main endoribonucleases responsible of tRNA cleavage activity for tRFs production. In plants grown under phosphate starvation conditions, the induction of RNS1 is correlated with the accumulation of specific tRFs. Beyond plants, we also provide evidence that short tRFs can be produced by the yeast Rny1p and that, in vitro, human RNase T2 is also able to generate long and short tRFs. Our data suggest an evolutionary conserved feature of these enzymes in eukaryotes.
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PMID:Plant RNases T2, but not Dicer-like proteins, are major players of tRNA-derived fragments biogenesis. 3046 57