Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:Q8IXL6 (
RNS
)
1,091
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A selective fluorogenic boronate-based probe-lactulose complex was evaluated for the rapid analysis of peroxynitrite (ONOO
-
) based on a reaction-based indicator displacement assay (RIA). The probe was synthesised by a simple nucleophilic substitution reaction between a boronic acid moiety and a well known laser dye,
DCM
. Fluorescence analyses showed that the probe had an off-on response to lactulose, forming a fluorogenic probe-lactulose complex. The subsequent addition of ONOO
-
selectively quenched the fluorescence of the complex over other Reactive Oxygen/ Nitrogen Species (ROS/
RNS
) tested. The complex can be applied for the rapid determination of ONOO
-
in full aqueous solution with good linear range, and has also proven suitable for monitoring ONOO
-
in living cells and real water samples.
...
PMID:Fluorogenic boronate-based probe-lactulose complex for full-aqueous analysis of peroxynitrite. 2815 3
