Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:Q8IXL6 (
RNS
)
1,091
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Bovine viral diarrhea virus (BVDV) entry into a host cell is mediated by the interaction of the viral glycoprotein E2 with the cellular transmembrane
CD46
receptor. In this study, we generated a stable Madin-Darby Bovine Kidney (MDBK)
CD46
-knockout cell line to study the ability of different
pestivirus A
and
B
species (BVDV-1 and -2) to escape
CD46
-dependent cell entry. Four different BVDV-1/2 isolates showed a clearly reduced infection rate after inoculation of the knockout cells. However, after further passaging starting from the remaining virus foci on the knockout cell line, all tested virus isolates were able to escape
CD46
-dependency and grew despite the lack of the entry receptor. Whole-genome sequencing of the escape-isolates suggests that the genetic basis for the observed shift in infectivity is an amino acid substitution of an uncharged (glycine/asparagine) for a charged amino acid (arginine/lysine) at position 479 in the E
RNS
in three of the four isolates tested. In the fourth isolate, the exchange of a cysteine at position 441 in the E
RNS
resulted in a loss of E
RNS
dimerization that is likely to influence viral cell-to-cell spread. In general, the
CD46
-knockout cell line is a useful tool to analyze the role of
CD46
for pestivirus replication and the virus-receptor interaction.
...
PMID:A CRISPR/Cas9 Generated Bovine CD46-knockout Cell Line-A Tool to Elucidate the Adaptability of Bovine Viral Diarrhea Viruses (BVDV). 3278 7
