Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q86TM3 (cage)
 29,987 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Radiation inactivation was used to estimate the molecular weight of the benzodiazepine (BZ), gamma-aminobutyric acid (GABA), and associated chloride ionophore (picrotoxinin/barbiturate) binding sites in frozen membranes prepared from rat forebrain. The target size of the BZ recognition site (as defined by the binding of the agonists [3H]diazepam and [3H]flunitrazepam, the antagonists [3H]Ro 15-1788 and [3H]CGS 8216, and the inverse agonist [3H]ethyl-beta-carboline-3-carboxylate) averaged 51,000 +/- 2,000 daltons. The presence or absence of GABA during irradiation had no effect on the target size of the BZ recognition site. The apparent molecular weight of the GABA binding site labelled with [3H]muscimol was identical to the BZ receptor when determined under identical assay conditions. However the target size of the picrotoxinin/barbiturate binding site labelled with the cage convulsant [35S]t-butylbicyclophosphorothionate was about threefold larger (138,000 daltons). The effects of lyophilization on BZ receptor binding activity and target size analysis were also determined. A decrease in the number of BZ binding sites (Bmax) was observed in the nonirradiated, lyophilized membranes compared with frozen membranes. Lyophilization of membranes prior to irradiation at -135 degrees C or 30 degrees C resulted in a 53 and 151% increase, respectively, in the molecular weight (target size) estimates of the BZ recognition site when compared with frozen membrane preparations. Two enzymes were also added to the membrane preparations for subsequent target size analysis. In lyophilized preparations irradiated at 30 degrees C, the target size for beta-galactosidase was also increased 71% when compared with frozen membrane preparations. In contrast, the target size for glucose-6-phosphate dehydrogenase was not altered by lyophilization.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Radiation inactivation studies of the benzodiazepine/gamma-aminobutyric acid/chloride ionophore receptor complex. 298 6

The effect of swimming instantly following an injection of isoproterenol was studied in rats in whom the administration of the drug caused changes in the cardiac muscle. The animals were compared with those of a group kept only under normal cage activity after the administration. Oxygen inhalation in the exercised group of rats was higher than in the sedentary group and after 4 minutes of measuring, continually remained at a higher level. It was determined that swimming prevented the early death of the rats. This was more pronounced in the animals of higher body weight. The extent of myocardial damage in the exercised group was markedly smaller. The intensity of enzyme reaction, SDH, LDH and G6PDH, in the area of undamaged tissue was the same in both the exercised and sedentary groups, in areas of damage it was minimal. The intensity of alpha GP was lowered in the exercised group, but in the sedentary group it was lowered throughout the entire section. Electron optic examination of the tissue, which under the light microscope appeared normal, revealed no substantial differences between the exercised and sedentary groups. We presume that the better condition of the rats was in part due to the peripheral breakdown of the catecholamine owing to the increased blood flow created by muscular activity.
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PMID:Influence of physical exertion on early isoproterenol-induced heart injury. 687 Jul 43