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Query: UNIPROT:Q86TM3 (cage)
 29,987 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Monoclonal or polyclonal antibodies to trophoblast components can be used to identify specific antigens. Such a vaccine will need to be effective before the completion of implantation and the appearance of the primitive streak about 14 days after fertilization. Monoclonal antibodies were evaluated at the Institute of Primate Research from WHO Panels and ones produced locally. Based on the results of placental reactivity, 12 antibodies were selected for further study and screened against selected baboon tissues. Monoclonal antibody T75 had the best specificity. A laparoscopic-guided uterine flush system was developed to replace the existing non surgical flush method in an effort to improve on the embryo recovery rate. Female baboons were introduced into the male cage for 5 hours/day for 6.3 +or- 3.4 days at stage 2, 3, or 4. 30 baboons were used in this study, but the animals were not flushed when bilateral adhesions were evident and the cervix or uterus could not be cannulated. Successful uterine flushed with immediate and complete return of the cultural medium were don in 20 animals. Superovulation of the baboons was tried to raise more embryos available for screening monoclonal antibodies. 4 animals were injected with human M gonadotropin (hMG) to stimulate follicular growth followed by human chorionic gonadotropin (hCG) to induce ovulation. Animals were mated following injection of hCG and laparoscopy was performed on day 6 of estimated pregnancy. Although follicular growth was evident, ovulation was not successful. Only 1 embryo was recovered from the 4 animals flushed. The recovered embryos were used for developing a cryosectioning technique to provide sections for screening monoclonal antibodies against pre implantation trophectoderm. Of the 8 baboon antibodies screened only 3 E7 showed reactivity against pre implantation baboon embryos.
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PMID:Anti-trophectoderm vaccines: rationale and methods used for antigen identification and selection. 151 28

Five pairs of female Swiss-Webster mice were caged with 5 males (2 females/1 male). Eight females were inoculated orally with 2.6 x 10(5) Caryospora bigenetica oocysts either 3 days before mating, 3 days after mating (PMD), 9 PMD, or 16 PMD. The remaining 2 females were inoculated orally with Hanks' balanced salt solution and served as controls. One female from each cage delivered naturally at full term and the second female delivered by cesarean section on postmating day 18. The number of offspring per litter ranged from 7 to 12. One female produced a litter of 3 stillborn and 5 liveborn offspring. Seven of 8 female mice exhibited swollen muzzles and footpads 8 days after inoculation. Caryospora bigenetica was identified in tissues of muzzle, tongue, footpad, uterus, and placenta at necropsy. This is the first report of C. bigenetica in uterus and placenta. Clinical signs and tissue infections were not observed in control mice or in any offspring of the 10 female mice. This study presents evidence that C. bigenetica is not transmitted transplacentally.
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PMID:Investigation of transplacental transmission of Caryospora bigenetica (Apicomplexa: Eimeriidae) in mice. 163 30

Usefulness of the ultrasonographical device (USD) for early diagnosis of pregnancy and for observation of fetal growth was examined in the cynomolgus monkey (Macaca fascicularis). Firstly, 50 females, each of which was mated for 24 hours in an individual cage, were used. They were diagnosed for pregnancy by the use of USD 15 to 29 days and 35 days after mating. Then, 20 of them were judged to be pregnant. The early pregnancy diagnosis was based on the detection of gestational sac (GS) in the uterus. The GS was detected 20.0 +/- 1.8 days after mating. The duration from the day on which GS was first detected to the day on which the heart beat of embryo (HBE) was detected averaged 5.6 +/- 1.5 days. In 17 of these 20 pregnancies, GS was detected 17 to 23 days after mating. As regards the remaining three pregnant cases, GS and HBE were simultaneously detected on the 28th day of pregnancy in two cases, and on the 35th day in one case. Secondly, 225 females who were mated by the routine 3 day-mating system were used. Pregnancy diagnosis by USD was conducted on the 21st, 28th and 35th day after mating. Ninety-six of the 225 females were judged to be pregnant. In 33 (34.1%) of the 96 pregnant cases, GS was detected on the 21st day of pregnancy. In 57 females (59.4%), GS was detected on the 35th day. Furthermore, it was able to judge conditions of fetal growth by measuring the size of GS and biparietal diameter.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Early pregnancy diagnosis by the ultrasonographical device and observation of fatal growth in cynomolgus monkeys (Macaca fascicularis)]. 331 2

Two experiments compared performance traits, feather loss, and nervousness of hens kept in contrasting homogeneous and heterogeneous body weight groupings. In addition, 15-day periods of daily uterine palpating vs nonpalpating for an egg in the uterus were compared for effects on egg production. Eighteen-wk-old pullets were weighed and characterized as light, medium, or heavy and housed in laying cages at 19 wk for a 50-wk production period. In Experiment 1, two hens were placed in each cage and there were three treatment groups: both light (L), mixed or 1 heavy and 1 light (X), and both heavy (H). In Experiment 2, three pullets were used per cage; a medium-weight group (M) was included and mixed cages included 1 heavy, 1 medium, and 1 light weight bird. In an ancillary experiment, 50-wk-old hens were also palpated or not palpated for an egg in the uterus over a single 15-day period. Results indicated that differences that were present in initial body weight groups persisted over the 50-wk period in Experiments 1 and 2 with no effect of weight grouping on body weight gain, egg production, and livability. Differences that were associated with weight groups were: age at sexual maturity, egg weight, egg mass, and nervousness score. No differences in mean feather scores were present among weight groups. Comparisons between individual hens of the same relative weight but kept in homogeneous or heterogeneous weight groups failed to indicate competitive advantages or disadvantages in egg production, feathering, and nervousness scores. No significant associations were found between nervousness scores of individual hens and other traits except for total body weight gain in Experiment 1, in which individuals that gained more were less nervous in the X groups (r = -.55). Palpating hens daily for two 15-day periods significantly increased hen-day egg production in Experiment 2 and nonsignificantly in Experiment 1, with no influence of palpation on hen-day egg production, egg weight, or egg mass in the ancillary experiment.
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PMID:Effects of body weight groupings on productivity, feather loss, and nervousness of caged hens. 368 51

Two hundred and thirty-six cases of multiple primary cancer associated with hematological malignancies, collected from 35 medical institutions in Japan, are reported. Based on the time interval between the first cancer and the second cancer, they were divided into three groups: synchronous cancer (94 cases), metachronous cancer subsequent to hematological malignancy (61 cases) and metachronous hematological malignancy subsequent to carcinoma (76 cases). The most common initial cancers were acute leukemia (including atypical leukemia and erythroleukemia), non-Hodgkin's lymphoma, multiple myeloma and chronic myelogenous leukemia of the hematological malignancies, and gastric cancer of the carcinomas. Patients with cancer of the uterus and breast in the metachronous cancer group metachronously developed hematological malignancies more frequently than those in the synchronous cancer group. Multiple primary cancer was observed more frequently in men than in women both in the synchronous cancer group and in the group with metachronous cancer subsequent to hematological malignancies. Acute leukemia was the most frequent disease type in incidence among the metachronous hematological malignancies. This secondary acute leukemia was characterized by a mostly granulocytic nature, poor response to chemotherapy and poor prognosis.
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PMID:Multiple primary cancers associated with hematological malignancies. 400 83

Postweaning development was monitored in domesticated rabbits reared in single- or mixed-sex groups at a commercial farm. The results suggest that sex composition of cage groups had no significant effect on weight gain, feed intake or mortality rates from weaning (35 days) to marketing (93 days). On sacrifice at 93 days, females from single- and mixed-sex groups showed no significant differences in plasma gonadotropin levels (LH and FSH) or weights of paunched carcase, ovaries, uterus, adrenals, kidneys or kidney fat. Evidence for within-cage dominance hierarchies was found at 63 days of age when 46% of the rabbits inspected showed signs of aggressive attack.
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PMID:Effects of single- and mixed-sex caging on postweaning development in the rabbit. 654 Mar 34

The present study was undertaken to assess the role of tumor-associated urokinase-type plasminogen activator (uPA) and its inhibitor type 1 (PAI-1) as a predictor for early relapse and poor prognosis in patients with stage II cervical cancer of the uterus. We have investigated the localization of uPA and PAI-1 immunohistochemically in formalin-fixed paraffin-embedded tissue sections. uPA and PAI-1 were analyzed antigenically, enzymologically, and zymographically in 28 patients with pelvic lymph node involvement and in 34 cases without nodal spread, as well as in 10 cases with normal cervix. In cancer tissues, strong staining for uPA was found in areas with invasive growth and degradation of surrounding normal tissue, while most tumor nests showed a mild or a moderate, evenly distributed PAI-1 staining. A significantly higher lymph node-positive rate was observed in patients having tumors with strong uPA and/or PAI-1 stainings than in those with tumors with weak stainings. In spite of significantly higher PAI-1 levels in the primary neoplastic tissues, uPA was found to be increased as well, both in antigen level and in activity. Most of PAI-1 obtained from cancer extracts is the latent form. These results suggest that cancer-associated increase in uPA seems not to be affected (or inhibited) by PAI-1 in areas where tumor cells are invading normal tissue. The overall survival and progression-free survival rate was worst in patients with the strong uPA staining confined to the tumor stromas and also with the strong PAI-1 staining at tumor nests, indicating that the greater localization of uPA in stromal cells than in malignant cells is a predictor of early relapse and poor prognosis in patients with cervical cancer of the uterus. Thus, the staining intensities and the localization of uPA and PAI-1 in tissue specimens appear to be predictors of increasing risk for lymph node metastasis, suggesting that some tumor cells recruit stromal cells to produce uPA and that PAI-1 may not act as a defense mechanism for tumor cell invasion and metastasis in the leading edge of tumor growth.
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PMID:Impact of urokinase-type plasminogen activator and its inhibitor type 1 on prognosis in cervical cancer of the uterus. 798 54

Compression of the fetal thorax by the uterine wall is one of the theories proposed to explain the mechanism of lung hypoplasia in association with oligohydramnios. However, the reduction in the intrathoracic space and the evidence of compression on the fetal thorax have not been demonstrated in detail. Therefore an experimental study was planned to determine the effects of oligohydramnios on available intrathoracic space and lung volume in the rabbit fetus. Amniotic fluid was shunted into the peritoneal cavity between 23 and 31 days of gestation. Intrathoracic space and lung volumes (TV and LV), and lung and body weight (LW and BW) values were measured in our study and control fetuses at term. Lung weight/body weight, lung volume/thorax volume, thorax volume/body weight ratios were calculated and the values of the fetuses with shunting and controls were compared. The difference between TV/BW ratios was not significant. LW/BW and LV/TV ratios were significantly reduced in experimental fetuses compared to the controls. Chronic drainage of amniotic fluid into the peritoneal cavity resulted in reduced LW/BW and LV/TV ratio, but did not effect TV/BW ratio. The low LV/TV ratio in the fetuses with shunting despite similar TV/BW ratio suggested that the diaphragm had been elevated. This is also an explanation for the observation of reduced chest circumference and chest circumference/abdominal circumference ratio in prolonged premature rupture of the membranes in vivo. The developing fetal lungs would undergo not only transthoracic but also trans-abdomino-diaphragmatic compression. However, it is unlikely in the tubular shaped rabbit uterus. In oligohydramnios amniotic fluid dynamics or physical characteristics with its contents should be disturbed. A previous experimental study with herniation of the amniotic sac causing lung hypoplasia through reduced intraamniotic pressure without oligohydramnios also questioned the role of fetal compression. Although fetal lung development was significantly impaired, the thoracic cage was not affected by amniono-peritoneal shunting. Therefore, we suggest that lung hypoplasia resulted from another mechanism rather than thoracic compression.
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PMID:Is fetal thoracic compression really the underlying mechanism of lung hypoplasia in oligohydramnios? An experimental study in a rabbit model. 916 52

Penetration of light into the pregnant sheep uterus was studied in 9 ewes, gestational ages 40 to 142 days (term 147 days). Light sensors were placed inside the pregnant horn and over the flank skin overlying the position of the uterine horn. To perform the experiments, the ewes were placed in a study cage outdoors and light sensors were connected to a luxometer. Simultaneous measurements were obtained from the intrauterine and the external sensors in the shade at noon. The amount of light detected inside the uterus increased with gestational age from two lux at 40 days to 51.1 +/- 16.5 (n = 5) lux at 142 days (0.2 and 5.4% of the amount of light detected at the maternal flank). Measurements through the 24 h were done in four pregnant ewes at 142 days gestation under natural photoperiod (13.5 light:10.5 dark). In these experiments, the intensity of intrauterine light changed through the 24 h, reflecting the changes in the intensity of the sunlight. Maximal intrauterine light values were observed at noon, corresponding to 4.7% of incident light. Small but detectable values were observed at 0900 and 1800 h. Our data show that, at mid gestation, light reaches the pregnant uterus and that, at late gestation, changes in intrauterine lighting throughout the 24 h are present reflecting the changes in external daylight. Therefore the sheep fetus is exposed to light-dark transitions at dawn and dusk, and to a peak of light at midday.
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PMID:Diurnal changes in light intensity inside the pregnant uterus in sheep. 977 85

Heat-shock protein 43 (HSP43) of Caenorhabditis elegans is prominently expressed in the utse cell, which attaches the uterus to the hypodermis, the uv1 cells joining the vulva and the uterus, the spermathecal valve and junctions between cells of the spermathecal cage. In body-wall muscle, HSP43 forms a punctate pattern of circumferential lines, probably corresponding to regions where the hypodermis contacts the muscle cells.
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PMID:HSP43, a small heat-shock protein localized to specific cells of the vulva and spermatheca in the nematode Caenorhabditis elegans. 1088 Mar 38


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