Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q7LGC8 (HSD)
 3,196 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An enzymologic study of corpora lutea in early pregnant rats treated with abortifacient agents is presented. Prostaglandin F2 alpha treatmen t (500 mcg twice daily 3 or 4 consecutive times) revealed an increase in corpora lutea glucose-6-phosphate dehydrogenase (G6PDH) activity of 110-140% and a moderate increase in 20 alpha-hydroxysteroid dehydrogenas e (20 alpha-HSD), whereas malic enzyme decreased to 27% of control values. Aminoglutethimide treatment (10-20 mg twice daily 6 or 7 consecutive times) revealed decreased G6PDH and malic enzyme activities while 20 alpha-HSD activity was maintained at a very low level. Corpora lutea of these aborted rats revealed moderately active 20 alpha-HSD valu es and slightly higher than control values for G6PDH, whereas malic enzy me activity fell to lower levels. Clomiphene citrate treatment (.5 ml of 3 mg/ml or .5 ml plus .5 ml of 10 mg/ml progesterone) caused abortion within 63 hours postinjection. G6PDH, malic enzyme, and adenosine triphosphate (ATP) citrate lyase activities in these corpora lutea decreased to 66, 68, and 72% of control levels, respectively, while 20 alpha-HSD activity was maintained at a very low level. Activities of 3beta hydroxysteroid dehydrogenase, 6-phosphogluconate dehydrogenase and pyruvate kinase were not appreciably altered. These results indicate that at the beginning of luteolysis and fetal resorption the activities of steroidogenic enzymes decreased and 20 alpha-HSD was not yet activated. Therefore, G6PDH, malic enzyme, and ATP citrate lyase activities could be measured to gauge early changes of luteolysis.
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PMID:An enzymologic study of corpora lutea in early pregnant rats treated with abortifacient agents. 95 39

The major role of the corpus luteum is biosynthesis of progesterone. Luteal function has been investigated by following plasma progesterone concentrations and by studying ultrastructural and histochemical changes in corpora lutea. Recently, changes in enzyme activities concerned with formation and degradation of progesterone are taken into investigation in order to understand the regulation of luteal function. In rat ovaries, progestational potency of ovarian secretions has been regulated by the activity of 20 alpha-hydroxysteroid dehydrgoenase (20 alpha-HSD), Which catabolizes progesterone to 20 alpha-hydroxypregn-4-en-3-one, progestatinally inert steroid. In regressing corpora lutea, extensive conversion of progesterone to 20 alpha-hydroxypregn-4-en-3-one occurred with a marked increase in 20 alpha-HSD activity as well as a decrease in plasma progesterone concentrations. On the other hand, histochemical studies of glucose-6-phosphate dehydrogenase (G 6 PDH) and delta5-3beta-hydroxysteroid dehydrogenase (3 beta-HSD) have been investigated without any remarkable changes in corporalutea at their early stages of luteolysis. In the present study the activities of steroidogenic enzymes in corpora lutea of pregnant rats are measured after treatment with a variety of abortifacient drugs, and compared with those in corpora lutea of 1 day post partum rats which showed changes characteristic of spontaneous luteolysis. On days 7 to 9 of pregnancy, Wistar-strain pregnant rats were injected with either prostaglandin F2alpha (PGF2alpha), aminoglutethimide or clomiphene citrate (clomid). Animals were sacrificed 15 to 63 hrs. after the last injection, and implantation sites were inspected. Ovaries were removed, and corpora lutea dissected free, weighed and homogenized. The homogenate was centrifuged at 105,000g for 60 min. The supernatant solution was assayed for the activities of G 6 PDH, 6-phosphogluconate dehydrogenase (6 PGDH), malic enzyme, ATP citrate lysase, 20 alpha-HSD and pyruvate kinase. The pellet fraction was re-homogenized, and centrifugated 2,000 g for 5 min. The supernatant solution was used for the assay of 3 beta-HSD. Complete fetal resorption was observed in all rats treated with PGF2alpha, while 7 out of 15 rats (47%) treated with both PGF2alpha and LH-RH maintained pregnancy. In intact rats after treatment with both drugs, lutein cells showed ultrastructures characteristic for luteolysis, although the degree of luteolysis was greatly diminished compared with PGF2alpha-treated ones. In agreement with these ultrastructural findings, 20alpha-HSD activity in corpora lutea was maintained at a rather low level in intact rats, while it was increased moderately in aborted ones after treatment with both drugs. In PGF2alpha-treated rats, G 6 PDH activity increased to 140% and malic enzyme activity decreased to 27% of the activity in control rats. In aminoglutethimide-treated rats, the activites of G 6 PDH and malic enzyme were decreased, while 2-alpha-HSD activity was maintained at a low level...
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PMID:[Studies on the activities of steroidogenic enzymes in corpora lutea of early pregnant rats treated with abortifacient drugs (author's transl)]. 124 45

Human meibomian glands were treated for the histochemical demonstration of several enzymatic activities. The 3 alpha-hydroxysteroid dehydrogenase (3 alpha-HSD), 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) appeared intensely reactive in the differentiating excretory cells, and weakly reactive in the basal cells and in the epithelial cells of the proximal portion of the ducts. The results indicate that meibomian glands can metabolize androgens by the reductive pathway, characteristic of target tissues. The finding of an intense reactivity for the enzymes glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) is also discussed.
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PMID:Human meibomian glands: a histochemical study for androgen metabolic enzymes. 233 45

Human parotid and submandibular glands showed no 3 alpha-hydroxysteroid dehydrogenase (3 alpha-HSD) activity. The 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and the 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) appeared intensely reactive in the duct epithelia of the male and female glands and weakly reactive in the acinar cells of the female ones. The failure to demonstrate 3 alpha-HSD activity indicates that in-vivo androgen activation, if present at all, is not so marked as in target organs. The different distribution of the 3 beta-HSD and 17 beta-HSD in the two sexes can be related not only to the oxidation of androgens but also to the metabolism of the female hormones. Glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) do not seem to be specifically influenced by the sex hormones as their pattern of distribution showed no sex differences.
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PMID:Histochemistry of the 3 beta-hydroxysteroid, 17 beta-hydroxysteroid and 3 alpha-hydroxysteroid dehydrogenases in human salivary glands. 695 69