Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:Q3SYG4 (C18)
 23,707 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Determination of amino acids in pig plasma with the classical ninhydrin system is influenced by the excessive amount of protein and lipophilic compounds in the sample, leading to a decline in resolution. This problem was eliminated by using 80 mg of sulphosalicylic acid per ml of plasma, and solid-phase extraction with a C18 cartridge as an additional clean up step. The latter resulted in significantly higher quantities of threonine, asparagine, glutamic acid, glutamine, glycine, alanine, valine and lysine, and lower levels of phenylalanine and tryptophan (P < 0.05). The use of a C18 cartridge had a minor effect on the analytical error.
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PMID:Modification of the analysis of amino acids in pig plasma. 801 33

The photodegradation of tryptophan in oxygen saturated aqueous solution sensitized by riboflavin is accompanied by the generation of the following reactive oxygen species: 1O2, OH., H2O2 and O2.-. When parallel photodegradation experiments are run with 14C-riboflavin in one case and 14C-tryptophan in the other and the irradiation products are separated by Sephadex G-15 and C18-HPLC, the generation of the following species is detected: aggregate forms of riboflavin, indolic products associated to flavins, indolic products of molecular weight higher than tryptophan, formylkynurenine, and other tryptophan photodecomposition products. The significance of the riboflavin anion radical and tryptophan cation radical as intermediates in the photoproduct formation is discussed.
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PMID:Riboflavin-sensitized photoprocesses of tryptophan. 802 50

In studies to determine the cause or causes of the eosinophilia myalgic syndrome (EMS) and to monitor the purity of L-tryptophan preparations, an HPLC method has been developed for determining 1,1'-ethylidenebis(L-tryptophan) (EBT) in L-tryptophan (W) preparations. The W preparations are extracted with 0.1% trifluoroacetic acid (TFA) and filtered, and the EBT is purified by passage through a Sep-Pak C18 cartridge. The cartridge is washed with water and 6% acetonitrile in water, and EBT is eluted with methanol. The water-diluted eluate is then chromatographed on a silica-based, reversed-phase HPLC column with a gradient of water and 80% acetonitrile, both solvents containing 0.1% TFA. EBT absorbance is measured at 280 nm. The average recovery of EBT from L-tryptophan powder, spiked over the range 1.2-4.8 micrograms/g, was 91%. The limit of determination was approximately 0.6 micrograms/g. Sixteen test samples of W products manufactured by the company to which most of the cases of EMS have been traced contained > 70 micrograms of EBT/g. Three nonpatient-related test samples either did not contain EBT or contained < 2 micrograms of EBT/g.
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PMID:High-performance liquid chromatographic determination of 1,1'-ethylidenebis(L-tryptophan) in L-tryptophan preparations. 807 28

1. 5-Hydroxytryptamine (5-HT) is a well known neurotransmitter implicated in mental disorders, tryptophan (Trp) as amino acid precursor may be interesting. 2. A rapid and simple reversed-phase (mu Bondapac C18 as stationary phase) liquid chromatographic method with fluorimetric detection (excitation: 302 nm, emission: 340 nm) is described for the quantitation of 5-hydroxytryptamine (5-HT) and free tryptophan (Trp), in whole blood. 5-fluoro-dl-tryptophan (5-FdlT) was used as internal standard. The mobile phase was 0.01 M phosphate buffer (pH = 4.5) with 0.0025 M 1-heptane sulfonic acid and 15% methanol. 3. In blood of healthy subjects (n = 30), the concentrations were, for 5-HT: 258 +/- 68 ng/ml and for Trp: 5.7 +/- 1.02 micrograms/ml. 4. The assay was used to follow the effect of antidepressant drugs, as a 5-HT reuptake inhibitor (clomipramine) and a monoamine oxidase inhibitor (moclobemide). After 4 week treatments, we observed, with clomipramine by 7 patients (150 mg/day) a highly significant reduction (p < 0.01) in 5-HT levels (77% +/- 12) and with moclobemide by 8 patients (300 or 450 or 600 mg/day) a highly significant increase in 5-HT levels (from 16% to 300% according to the drug dosage). These two drugs did not influence blood Trp concentrations. 5. Assays on whole blood present various advantages: (i) the simplicity for clinical departments (ii) the absence of sample pretreatment and (iii) the assurance that the entire platelet population is assayed since all of the 5 HT in blood is bound to platelets.
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PMID:Determination of 5-hydroxytryptamine and tryptophan by liquid chromatography in whole blood. Its interest for the exploration of mental disorders. 811 74

Phosphatidylcholines have been synthesized containing a cholesterol moiety at the 2-position of the glycerol backbone. Fluorescence quenching studies show that cholesterol-containing phosphatidylcholines can bind at the lipid-protein interface of the Ca(2+)-ATPase from skeletal muscle sarcoplasmic reticulum, with an affinity half that of dioleoylphosphatidylcholine. The ATPase activity measured for the ATPase reconstituted with the cholesterol-containing phosphatidylcholine containing an oleoyl fatty acyl chain, (C18:1, CHS)PC, is less than that measured for the ATPase reconstituted with dioleoylphosphatidylcholine. The activity measured for the ATPase reconstituted with the cholesterol-containing phosphatidylcholine containing a myristoleoyl fatty acyl chain, (C14:1, CHS)PC, is less than that measured in (C18:1,CHS)PC and is comparable to that measured in dimyristoleoylphosphatidylcholine (di(C14: 1)PC. The stoichiometry of Ca2+ binding to the ATPase is two Ca2+ ions bound per ATPase molecule in the native membrane or in (C18:1,CHS)PC, but one bound per ATPase molecule in di(C14:1)PC or (C14: 1,CHS)PC. Addition of cholesterol to the ATPase in di(C14:1)PC or (C14:1,CHS)PC increases the Ca2+ binding stoichiometry to the usual 2:1, but the binding stoichiometry remains 1:1 in mixtures of di(C14: 1)PC and (C14:1,CHS)PC. Removal of Ca2+ from the Ca(2+)-bound ATPase results in a decrease in tryptophan fluorescence intensity for the ATPase in the native membrane, but an increase in fluorescence intensity for the ATPase in di(C14:1)PC or (C14:1,CHS)PC. Addition of cholesterol to the ATPase in di(C14:1)PC or (C14:1,CHS)PC reverses this change. It is concluded that cholesterol linked to a phospholipid molecule can interact with the ATPase only at the lipid-protein interface.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Binding sites for cholesterol on Ca(2+)-ATPase studied by using a cholesterol-containing phospholipid. 816 59

A substance that exhibited a tryptophan-like fluorescence peak at 354 nm on excitation at 295 nm at neutral pH was isolated from human urine. This compound was determined by visible-light absorption spectroscopy, fluorescence spectroscopy, 1H and 13C NMR spectroscopies, and FAB-MS to be 1-(1',2',3',4',5'-pentahydroxypentyl)-1,2,3,4-tetrahydro-2-carboli ne-3- carboxylic acid. This compound, named tetrahydropentoxyline, is a new type of hydrophilic tetrahydro-beta-carboline, and its elution position was between those of 4-pyridoxic acid and kynurenic acid on C18 reversed-phase HPLC. The amount of tetrahydropentoxyline excreted in the urine of normal subjects [n = 21; age, 45 (SD 20) years] was about 5.2 (SD 1.0) mg per day.
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PMID:A hydrophilic tetrahydro-beta-carboline in human urine. 820 87

Liver tryptophan 2,3-dioxygenase (TDO) activity was determined by high-performance liquid chromatography. The enzyme activity was expressed as the sum of N-formyl-L-kynurenine (FK) and L-kynurenine (KYN) produced from L-tryptophan (TRY) by liver slices. FK and KYN were detected spectrophotometrically at 254 nm after their separation on a reversed-phase C18 column. KYN formation proceeded according to zero-order kinetics for at least 4 h with 15 mM TRY at 37 degrees C. The apparent Michaelis constant was 1.2 mM TRY with a maximum velocity of 59 pmol min-1 mg-1 wet weight. The method was applied for TDO assay in mice treated with the organophosphorus acid triester diazinon. Kynurenine formamidase inhibition by diazinon resulted in reduced KYN formation, FK accumulation, and moderate TDO increase.
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PMID:Assay of tryptophan 2,3-dioxygenase using liver slices and high-performance liquid chromatography. 831 34

A high-performance liquid chromatographic (HPLC) profiling method was developed to separate trace impurities in L-tryptophan products associated with the eosinophilia-myalgia syndrome (EMS) epidemic. The test portion was dissolved in water, and the solution was filtered and chromatographed on a silica-based C18 reversed-phase HPLC column by using linear gradient elution with water and acetonitrile-water (80:20); both solvents contained 0.1% trifluoroacetic acid for ion-pairing. The method was used to profile 200 test samples from six manufacturers of L-tryptophan. The method was modified to include the use of a C18 disposable cartridge to retain the 1,1'-ethylidene-bis(L-tryptophan) (peak E, peak 97 or EBT), the impurity most strongly associated with EMS, and to remove the L-tryptophan before HPLC separation and quantitation. Recoveries of EBT added to test portions (2 micrograms/g and above) averaged 80%.
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PMID:Separation and isolation of trace impurities in L-tryptophan by high-performance liquid chromatography. 844 99

We developed a simple and sensitive assay for the 8-methyl ether of xanthurenic acid in serum by high-performance liquid chromatography with fluorescence detection (excitation at 340 nm, emission at 450 nm). The compound under study in serum samples was extracted with Sep-Pak C18 cartridge and the extract was applied to an octadecylsilane-bonded column (Nucleosil 5C18, 150 x 4 mm I.D.). The mobile phase used was a mixture of 0.05 M sodium acetate buffer (pH 6.0), containing 5 mM sodium 1-octanesulfonate and 0.1 mM Na2EDTA, and acetonitrile (93:7, v/v). The 8-methyl ether of xanthurenic acid from serum samples was sufficiently separated to be clearly distinguishable. The quantification limit was 2 x 10(-14) mol, which was sensitive enough to detect 8-methyl ether of xanthurenic acid in serum from normal subjects. The method was applied to samples from patients with deficiency in tryptophan metabolism, xanthurenic acid/3-hydroxy kynurenineuria and showed a striking elevation in serum 8-methyl ether of xanthurenic acid.
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PMID:Determination of the 8-methyl ether of xanthurenic acid in human serum by high-performance liquid chromatography with fluorescence detection. 899 63

We propose the name Pseudomonas monteilii for a new species of gram-negative, rod-shaped, motile bacteria that were nonhemolytic on blood agar and were isolated from clinical sources. The 10 strains of P. monteilii were incapable of liquefing gelatin. They grew at 10 degrees C but not at 41 degrees C, produced fluorescent pigments, catalase, and cytochrome oxidase, and possessed the arginine dihydrolase system. They were capable of respiratory but not fermentative metabolism. They did not hydrolyze esculin or starch and were able to use benzylamine, alpha-aminobutyrate, D-ribose, L-arabinose, butyrate, valerate, isovalerate, isobutyrate, inositol, phenylacetate, D-alanine, and amylamine. They possessed L-phenylalanine arylamidase, L-lysine arylamidase, L-alanine arylamidase, gamma-glutamyl-transferase, glycyl-phenylalanine arylamidase, L-tryptophan arylamidase, glycyl-L-alanine arylamidase, esterase C4, esterase C6, esterase C8, esterase C9, esterase C10, and esterase C18. DNA relatedness studies revealed that P. monteilii strains formed a homogeneous DNA hybridization group. A total of 57 strains representing previously described or partially characterized taxa belonging to the genus Pseudomonas were 6 to 54% related to P. monteilii. The highest hybridization values were obtained with strains belonging to or related to Pseudomonas putida biovar A. The average G+C content of the DNA was 60.5 +/- 0.5 mol% for four of the P. monteilii strains studied. The type strain of P. monteilii is CFML 90-60 (= CIP 104883); it was isolated from bronchial aspirate and has a G+C content of 60 mol%. The clinical significance of these organisms is not known.
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PMID:Pseudomonas monteilii sp. nov., isolated from clinical specimens. 922 17


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