Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:Q16637 (SMA)
 8,107 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Serum gamma-glutamyl transferase (GGT, EC. 2.3.2.2. was measured in 173 patients with diseases of the hepatobiliary system (including metastatic cancer) and in 90 patients who were subsequently shown to have primary diseases of other etiology. All patients had been selected because they had abnormal alkaline phosphatase, aspartate aminotransferase or bilirubin on SMA 12/60 screening. Serum GGT was elevated in 97% of patients with primary hepatobiliary disease. The magnitude of the increase in GGT was variable in all groups and was unhelpful in differential diagnosis, even between medical and surgical cases. Moreover, GGT was abnormal in 69 patients who did not have primary hepatobiliary disease (77%), an incidence higher than that for other enzyme tests performed. We conclude that because GGT was more susceptible than other tests to spurious elevation in the absence of hepatobiliary disease and was unhelpful in differential diagnosis, it has little value apart from monitoring alcohol abuse and enzyme induction.
 ...
PMID:Lack of value of serum gamma-glutamyl transferase in the diagnosis of hepatobiliary disease. 3 86

We evaluated a new multiple-channel chemistry analyzer, the Beckman "Astra-8," which is controlled by a microprocessor with 52K bytes of memory. The instrument we tested performed the following tests: Na, K, CO2, chloride, urea nitrogen, glucose, and creatinine. We compared the Astra-8 to a continuous-flow (SMA 6/60) and a discrete (Du Pont aca, urea nitrogen only) analyzer. The Astra-8 demonstrated excellent precision, linearity, accuracy, analytical recovery, lack of interference, ease of operation, and satisfactory comparison to values obtained by the comparison methods.
 ...
PMID:Evaluation of a new multichannel analyzer, "Astra-8". 71 58

We present performance studies on the Technicon "SMAC" analyzer. Precision was estimated from control data during routine operation and from blind duplicates of sera from patients. Patients' specimens were analyzed with the SMAC and the SMA 12/60 during initial evaluation studies. Comparisons were also made with the Du Pont aca and other methods during routine service. Performance is judged relative to medical usefulness requirements, by using previously developed criteria and recommendations for decision levels and allowable errors.
 ...
PMID:Performance studies on the Technicon "SMAC" analyzer: Precision and comparison of values with methods in routine laboratory service. 125 32

We describe a method for the determination of serum albumin on the SMA 12/60 that utilizes the anionic dye bromocresol purple (BCP). This dye is more specific than the commonly used bromocresol green (BCG) in that it does not bind to globulins. The SMA module requires two simple changes for the BCP method to be adopted: a 600 nm interference filter replaces the 630 nm filter; and a gray-gray pump tube (rated at 1.00 mL/min) replaces a green-green tube (rated at 2.00 mL/min) in the predilution assembly. The precision of this method is comparable to the BCG method with a CV of 2.9% at mean values of 30.6 and 38.3 g/L. This method was compared to the BCP method of the DuPont aca using 51 patient samples over an approximate albumin range of 15 to 80 g/L. The correlation is very good as indicated in the linear regression data: (SMA) = 0.884(aca) + 3.46; coefficient of determination (r2) is 0.986; and standard error of the estimate (Sy.x) is 1.39 g/L. Neither hemoglobin nor bilirubin interferes with this BCP method up to levels of 9 g/L and 633 mumol/L, respectively.
 ...
PMID:Bromocresol purple dye-binding method for the estimation of serum albumin adapted to the SMA 12/60. 405 41

We evaluated a new method for the determination of albumin on the DuPont aca discrete clinical analyzer that utilized an albumin selective dye, bromocresol purple (BCP). This dye minimizes globulin interference that occurs with bromocresol green (BCG) methods that have long (greater than 30 s) incubation times. Good correlation was observed between the new BCP method and two methods which do not show significant globulin interference: an immunological method and a rapid BCG method. Comparisons with alternate BCG methods (SMA 12/60, SMAC, and the aca) were generally not as good. The albumin results by the BCP method on specimens with elevated globulins were similar to results from the immunological and rapid BCG methods. The reference interval was 34-50 g/L. The between-day coefficient of variation ranged from 0.9 to 3.5% at three evaluation sites on 2 levels of 8 different control materials. The method was linear between 6-70 g/L. No interference was observed from hemoglobin at levels less than or equal to 5.00 g/L or from bilirubin at levels less than 342 mumol/L. Also, no interference was observed from nine common drugs that are known to bind to albumin. These studies show that accuracy, reproducibility and linearity of the BCP albumin method on the aca are acceptable for clinical use.
 ...
PMID:Evaluation of a bromocresol purple method for the determination of albumin adapted to the DuPont aca discrete clinical analyzer. 405 42

The aim of the investigation was to estimate chosen parameters of fat balance and immunological reactivity in patients with virushepatitis and alcohol abuse (30 patients--I group), and without alcohol abuse in anamnesis (30 patients--II group). The investigations included the estimation of the presence of HBsAg in serum, the level of immunoglobulins IgG, IgA and IgM, the determination of T and B lymphocytes, the presence of organ-autoantibodies (SMA, ANA, AMA), the level of free fatty acids, triglycerides, full lipids, beta-lipoproteins and cholesterol in the blood serum. The rise in full lipids content in the blood serum, beta-lipoproteins, triglycerides, the increase of IgA level, the decrease of lymphocytes T percentage, more frequent confirmation of the presence of antimitochondrial autoantibodies were noted, especially in the group of patients with virushepatitis and alcohol abuse. Disturbances in fat balance remain in the convalescence period.
 ...
PMID:[Lipid values and immune parameters in the course of viral hepatitis in patients with alcohol abuse and without alcohol stress in anamnesis]. 633 26

The bromocresol purple (BCP) albumin method on the DuPont aca was evaluated by determining the accuracy and precision of the method. A split sample comparison was performed against the electroimmunoassay (EIA) method as well as the bromocresol green (BCG) method on the DuPont aca and the Technicon SMA 12/60. The BCP method was found to have more than adequate precision and its accuracy is as good as the EIA method. The BCP method will give results which are approximately 9 g/L lower than end point BCG methods. Problems have been identified with some of the commercial materials that could be used to standardize the BCP method, since different commercial preparations of human serum albumin do not react in identical fashion in the BCP and EIA methods. For this reason caution should be exercised when standardizing the BCP method.
 ...
PMID:Evaluation of the Dupont aca bromocresol purple albumin method. 634 50

Total protein concentration in the serum of a patient with hyperviscosity syndrome differed as measured by the biuret procedure in the DuPont aca (80 g/L) and the SMA 12/60 (105 g/L), owing to viscosity-dependent errors with the aca sampling system; the magnitude depended on sample temperature and volume of sample aspirated. This kind of error was not observed with the SMA 12/60 and was far less severe when a Micromedic sampler-dilutor was tested. It could be eliminated in the case of the aca by adding sample to test packs with a syringe rather than with the aca automated sampler-dilutor. We thus recommend use of the syringe method when unusually viscous samples (serum or other body fluids) are analyzed in the aca.
 ...
PMID:Sample viscosity can be a source of analytical error when discrete sampler-dilutors are used. 679 49

We evaluated the performance of the Kodak Ektachem 400 Analyzer in a 16-week clinical trial. We assessed four potentiometric tests and nine colorimetric tests for precision and correlation with results obtained some other commonly used instruments (Technicon SMA II and C800 System, Du Pont aca II, and Baker CentrifiChem). The comparison was favorable for all tests except albumin, sodium, and carbon dioxide. The reagents are stable. Little refrigerator storage space is needed. The computer system and the modular component design of the Ektachem 400 Analyzer make operation, troubleshooting, and preventive maintenance relatively easy.
 ...
PMID:Clinical evaluation of Eastman Kodak's Ektachem 400 Analyzer. 682 62

We evaluated the influence of hemolysis on total serum calcium as determined with the Du Pont aca, SMA 12/60, Ektachem 400, Corning 940 EGTA titrator, and the Beckman Astra 8, comparing results with those obtained by atomic absorption spectroscopy. We find that hemoglobin does not influence calcium measurements with the SMA 12/60, Ektachem 400, or Beckman Astra 8. The presence of hemoglobin exceeding 2 to 3 g/L caused falsely high results with the aca and falsely low results with the Corning 940 titrator. Similar interferences may be observed with other titrating or colorimetric procedures that involve direct reaction of the sample with o-cresolphthalein complexone or calcein. Upon removal of the hemoglobin by precipitation with trichloroacetic acid, the values obtained with the aca and the Corning 940 EGTA titrator were similar to those measured by atomic absorption. With nonhemolyzed serum samples the acid treatment had little or no effect on the aca procedure but resulted in a positive bias of approximately 10% with the EGTA procedure. Thus we recommend this trichloroacetic acid procedure for measuring calcium in hemolyzed samples with the aca and, with certain reservations, with the EGTA titrator.
 ...
PMID:Interference by hemolysis with various methods for total calcium and its correction by trichloroacetic acid precipitation. 687 9


1 2 Next >>