Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:Q06643 (non-Hodgkin's lymphoma)
 11,307 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The reduction of residual tumor cells is one of the main targets of leukapheresis product (LP) processing. Immunomagnetic enrichment/selection of CD34+ progenitor cells (Baxter Isolex 300i) can achieve a reduction of contaminating B-cells of approximately 2-3 logs in B-cell non-Hodgkin's lymphoma patients. Specific release of the enriched CD34+ cells (stem cell releasing agent PR34+; Baxter) and the use of antibody-coated immunobeads targeted against B-cell markers (CD10, CD19, CD20, CD22, CD23, and CD37) during this procedure allows the GMP-like simultaneous capture of residual B cells within a closed system. This combination of two purging techniques enhances the B-cell depletion capacity up to 4.5 logs. By performing 10 clinical-scale purging procedures, we could show that the simultaneous immunomagnetic purging method is easy to perform and highly efficient. We evaluated B-cell log depletion by flow cytometry for cases with marker-positive cells detectable before and after the purging procedure. The mean reduction of B-cells in these cases was 3.5 logs; the mean CD34+ cell yield and purity were 47 and 92%. Using three LPs, we tested the procedure on a modified Baxter Isolex 300i device with software adaptations for this procedure (software version 2.0) in direct comparison with CD34+ cell selection only, using the former version (version 1.12). The CD34+ cell yield was 49% (40-54%) for the CD34+ cell selection and 51% (19-72%) for simultaneous double selection. The mean purity was 96% for CD34+ cell selection and 98% for simultaneous double selection. B-cell depletion was 1.9 logs for CD34+ cell selection, and after simultaneous double selection, the B-cell content was decreased by 3.7 log steps (P = 0.0495). Clinical application of double-purged cells has not prolonged the hematopoietic recovery times after high-dose therapy as compared with nonpurged peripheral blood progenitor cell autotransplants. In conclusion, we could show that the simultaneous double selection protocol developed leads to a highly increased B-cell purging efficacy when compared with CD34+ cell selection without any negative effects regarding CD34+ cell yield and engraftment times after high-dose therapy.
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PMID:Simultaneous immunomagnetic CD34+ cell selection and B-cell depletion in peripheral blood progenitor cell samples of patients suffering from B-cell non-Hodgkin's lymphoma. 1120 18

Silver stained argyrophilic nucleolar organizer regions (AgNOR) had widely been used for diagnostic as well as prognostic purposes in surgical pathology. Our study was carried out for the quantitative and morphometric analysis of AgNORs in various lymph node lesions. Ten cases each of reactive lymphadenitis and non-Hodgkin's lymphoma (NHL) were included in the present study. Out of 10 cases of NHL, 5 cases each were of Low grade and High grade lymphomas. The morphometric analysis and quantification of AgNORs were curried out using a specific programme of an automatic image analysis Biovis Image measure version 1.0. The results revealed that mean AgNOR count was maximum in high grade lymphoma (5.17 +/- 0.255) when compared with low grade lymphoma (4.78 +/- 0.41) and reactive lymph nodes (2.45 +/- 0.486). Similarly, the mean of total AgNOR area in high grade lymphoma (511.9 +/- 41.52) was highest followed by low grade lymphoma (451.12 +/- 37.1) and reactive lymphadenitis (215 +/- 37). However, the mean of ratio of single AgNOR dot area to nuclear area was lowest in high grade lymphoma (0.0718 +/- 0.01) in comparison to low grade lymphoma (0.0826 +/- 0.0096) and reactive lymph node (0.132 +/- 0.01). Thus, it can be concluded from the present study that different features of AgNOR evaluated morphometrically (AgNOR count, total AgNOR area, AgNOR dot area, ratio of total AgNOR area and single AgNOR area to nuclear area) were significantly altered in various lymph node lesions which may be helpful in differential diagnosis of problematic cases.
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PMID:Morphometric analysis of AgNORs in the lymph node lesions. 1975 31