Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P62988 (
Ubiquitin
)
4,326
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ubiquitin
-dependent proteolysis of
cyclin
B and securin initiates sister chromatid segregation and anaphase. The anaphase-promoting complex/cyclosome and its coactivator CDC20 (APC/C
CDC20
) form the main ubiquitin E3 ligase for these two proteins. APC/C
CDC20
is regulated by CDK1-
cyclin
B and counteracting PP1 and PP2A family phosphatases through modulation of both activating and inhibitory phosphorylation. Here, we report that PP1 promotes
cyclin
B destruction at the onset of anaphase by removing specific inhibitory phosphorylation in the N-terminus of CDC20. Depletion or chemical inhibition of PP1 stabilizes
cyclin
B and results in a pronounced delay at the metaphase-to-anaphase transition after chromosome alignment. This requirement for PP1 is lost in cells expressing CDK1 phosphorylation-defective CDC20
6A
mutants. These CDC20
6A
cells show a normal spindle checkpoint response and rapidly destroy
cyclin
B once all chromosomes have aligned and enter into anaphase in the absence of PP1 activity. PP1 therefore facilitates the metaphase-to-anaphase transition by promoting APC/C
CDC20
-dependent destruction of
cyclin
B in human cells.
...
PMID:PP1 promotes cyclin B destruction and the metaphase-anaphase transition by dephosphorylating CDC20. 3275 77
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