Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P61278 (
somatostatin
)
22,083
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Corticotropin-releasing hormone-binding protein
(
CRH-BP
) is a secreted glycoprotein that binds CRH with very high affinity to modulate CRH receptor activity.
CRH-BP
is widely expressed throughout the brain, with particularly high expression in regions such as the amygdala, hippocampus, ventral tegmental area and prefrontal cortex (PFC). Recent studies suggest a role for
CRH-BP
in stress-related psychiatric disorders and addiction, with the PFC being a potential site of interest. However, the molecular phenotype of
CRH-BP
-expressing cells in this region has not been well-characterized. In the current study, we sought to determine the cell type-specific expression of
CRH-BP
in the PFC to begin to define the neural circuits in which this key regulator is acting. To characterize the expression of
CRH-BP
in excitatory and/or inhibitory neurons, we utilized dual
in situ
hybridization to examine the cellular colocalization of
CRH-BP
mRNA with vesicular glutamate transporter (VGLUT) or glutamic acid decarboxylase (GAD) mRNA in different subregions of the PFC. We show that
CRH-BP
is expressed predominantly in GABAergic interneurons of the PFC, as revealed by the high degree of colocalization (>85%) between
CRH-BP
and GAD. To further characterize the expression of
CRH-BP
in this heterogenous group of inhibitory neurons, we examined the colocalization of
CRH-BP
with various molecular markers of GABAergic interneurons, including parvalbumin (PV),
somatostatin
(
SST
), vasoactive intestinal peptide (VIP) and cholecystokinin (CCK). We demonstrate that
CRH-BP
is colocalized predominantly with
SST
in the PFC, with lower levels of colocalization in PV- and CCK-expressing neurons. Our results provide a more comprehensive characterization of the cell type-specific expression of
CRH-BP
and begin to define its potential role within circuits of the PFC. These results will serve as the basis for future
in vivo
studies to manipulate
CRH-BP
in a cell type-specific manner to better understand its role in stress-related psychiatric disorders, including anxiety, depression and addiction.
...
PMID:Cell Type-Specific Expression of Corticotropin-Releasing Hormone-Binding Protein in GABAergic Interneurons in the Prefrontal Cortex. 2906 56
