Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P61278 (somatostatin)
 22,083 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In the chicken, serotonin-immunoreactive cells were widely distributed not only in the carotid body but also in the wall of the common carotid artery and around each artery arising from the common carotid artery. Almost all of the serotonin cells in the wall of the common carotid artery were intensely immunoreactive to the neuropeptide Y, met- and leu-enkephalin antisera, whereas in the carotid body only a few cells were immunoreactive to these antisera. Innervation of the serotonin cells in and around arteries of chickens was investigated by immunohistochemistry and electron microscopy, in comparison with that of the carotid body. The serotonin cell groups in and around arteries, as well as the carotid body, received numerous peptidergic nerve fibers. Calcitonin gene-related peptide (CGRP)- and substance P-immunoreactive varicose nerve fibers were densely distributed, and somatostatin-immunoreactive fibers were moderately distributed in the serotonin cell groups. Galanin- and vasoactive intestinal peptide (VIP)-immunoreactive fibers were sparsely distributed in the cell groups. By electron microscopy, the serotonin cells in and around arteries were characterized by the presence of numerous dense-cored vesicles, 70-220 nm in diameter. The granule-containing cells were in close association with numerous axons. Naked axons regarded axon terminals were frequently apposed on the granular cells. The axon terminals were usually long and often partly invested the granular cells. Numerous synaptic junctions were detected along the contact between the granular cells and axon terminals. Most of the synaptic junctions showed afferent morphology; the secretory granules were accumulated near and attached to the asymmetrical membrane thickenings. Thus, the serotonin cells in and around arteries, like the carotid body, constitute chemoreceptive tissue.
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PMID:Innervation of the serotonin-immunoreactive cells distributed in the wall of the common carotid artery and its branches in the chicken. 232 11

The Mastomys (Praomys natalensis) species are a unique natural model in which the bioactivity of gastric carcinoids may be studied. Several investigators have previously demonstrated that these tumors contain large amounts of histamine. In this study we investigated the presence of peptides associated with the neoplasm. The levels and location of gastrin, gastric inhibitory peptide (GIP), neurotensin, peptide YY (PYY), pancreatic polypeptide (PP), glucagon, bombesin, vasoactive intestinal peptide (VIP) and somatostatin (SRIF) were investigated by radioimmunoassay and immunocytochemistry. In addition the distribution of these peptides were evaluated in the gastrointestinal tract of young and old animals to investigate possible age-related changes. PYY and enteroglucagon (EG) were significantly (P less than 0.001) elevated in both tumor tissue (676 +/- 152, 551 +/- 164 pmol/g) and plasma (620 +/- 160, 500 +/- 147 pmol/l) of tumor-bearing animals. Immunocytochemistry revealed PYY- and EG-like immunoreactivity in 20-30% of tumor cells. A significant decrease (P less than 0.05) in bombesin was noted in older animals, but no changes in gastric tissue content of PYY or EG could be detected between young and old animals. Gastrin was not detected in tumors and there were no significant changes in tissue or plasma levels with age. Small bowel concentrations of VIP and PYY were higher in the older mastomys (P less than 0.05). In contrast, colonic levels of bombesin, VIP, somatostatin and PYY were significantly lower (P less than 0.05) in older mastomys compared with young. The age-related changes in several peptides may reflect an adaptive response to acid hypersecretion. The multi-hormonal character of these neoplasms suggests that these tumors develop from a pluripotential stem cell.
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PMID:Significance of gastric endocrine tumor and age-related gut peptide alterations in Mastomys. 232 98

The ileocaecal junctions of 5 horses and 2 donkeys were examined by using antisera to the following peptides: somatostatin, glucagon, gastrin, neurotensin, vasoactive intestinal peptide (VIP), peptide histidine isoleucine (PHI), calcitonin gene-related peptide (CGRP), substance P (SP) and neuropeptide Y (NPY). Antisera to somatostatin, neurotensin and NPY demonstrated endocrine cells in the ileal- and caecal parts of the ileocaecal junction, while immunoreactivity for glucagon was demonstrated in endocrine cells of the ileal part only. Nerve cell bodies showing immunoreactivity to SP, VIP, CGRP and PHI were demonstrated in the myenteric and submucosal plexuses and were associated with small blood vessels in the submucosa of all the regions tested. Ramified nerve fibres in the submucosa immunoreactive to SP, VIP, CGRP and PHI extended to the mucosa and to small blood vessels in the submucosa. Nerve fibres showing immunoreactivity to SP, VIP and PHI extended to the circular smooth muscle layer of the ileocaecal junction.
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PMID:An immunohistochemical study of various peptide-containing endocrine cells and neurones at the equine ileocaecal junction. 233 94

Three distinct vesicle fractions enriched 40-60 times in the neuropeptides substance P, somatostatin, and vasoactive intestinal peptide (VIP) were prepared from the myenteric plexus of guinea pig ileum by density gradient centrifugation in a small zonal rotor. Mean densities (in g X ml-1) and diameters (in nm) of the three classes of vesicles were: substance P, 1.123, 65; somatostatin, 1.138, 37; VIP, 1.148, 110; standard deviations were about 5%. These peaks were distinct from the peak of acetylcholine-containing vesicles of density 1.066 g X ml-1 and diameter 61 nm. When a relatively mild method of homogenization was used a second peak of acetylcholine appeared in the same region of the gradient as VIP and the VIP was larger. This may represent a class of vesicles containing both acetylcholine and VIP, though cosedimentation of two classes of vesicles of almost the same density and similar fragility, one containing VIP and the other acetylcholine, cannot be entirely excluded on present evidence.
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PMID:Isolation of neuropeptide-containing vesicles from the guinea pig ileum. 240 27

The localization of Substance P(SP)-, Methionine-Enkephalin(met-Enk)-, Somatostatin(SOM)- Serotonin(SER)-, Cholecystokinin(CCK)-, and Vasoactive intestinal polypeptide (VIP)-like immunoreactivity (-LIR) has been determined immunocytochemically in the thoracic spinal cord intermediate zone of male and female guinea pigs. All neuroactive substances studied are exclusively localized in nerve fibre varicosities and terminals building up the vegetative network of the thoracic spinal cord intermediate zone. This network is situated dorsally to the central canal as a longitudinal plate of approximate thickness of 90-100 microns. Immunoreactive fibres are observed in the two Fasciculi longitudinales laterales and the two Fasciculi longitudinales mediales which are interconnected by transverse and oblique peptide-containing bundles (the terminology used by Petras and Cummings 1972; Galabov and Davidoff 1976). All these bundles interconnect the nuclei intermediolaterales principales and funiculares, the nuclei intercalates spinales and the nuclei intercalates paraependimales in ipsi- and contralateral as well as in rostral and caudal direction. The neurones of these nuclei are surrounded by immunoreactive varicosities and terminals. The quantity of the immunoreactive structures and intensity of the staining varied for the different neuroactive substances. As to the origin of the vegetative network immunoreactive fibres three main possibilities exists: a). From primary afferent neurones situated in the dorsal root ganglia, which send their axons via the dorsal roots (mainly for SP and perhaps for CCK); b). From supraspinal neurones which send their axons descending in the white matter funiculi and in the fasciculi longitudinales laterales and mediales and c). From intrinsic spinal cord neurones, which send their neurites in ascending and descending directions, ipsi- and contralaterally and interconnect the spinal cord segments. The different origin of the vegetative network immunoreactive fibres as well as the complex innervation of the preganglionic sympathetic nerve cells in the intermediate zone of the spinal cord suggests that this network may play an important role in the integration of the central and peripheral vegetative nervous system as well as probably in the integration of the somatic and the vegetative nervous system.
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PMID:Localization of some neuropeptide- and serotonin-like immunoreactivities in the vegetative network of guinea pig spinal cord. 241 Apr 87

The distributions of peptide-containing nerve fibers and cell bodies in the canine small intestine were determined with antibodies raised against seven peptides: enkephalin, gastrin-releasing peptide (GRP), neuropeptide Y, neurotensin, somatostatin, substance P, and vasoactive intestinal peptide (VIP). Immunoreactive nerve cell bodies and fibers were found for each peptide except neurotensin. In the muscle layers there were numerous substance P, VIP, and enkephalin fibers, fewer neuropeptide Y fibers, and very few GRP or somatostatin fibers. The mucosa contained many VIP and substance P fibers, moderate numbers of neuropeptide Y, somatostatin, and GRP fibers and rare enkephalin fibers. Nerve cell bodies reactive for each of the six neural peptides were located in both the myenteric and submucous plexuses. The distributions of nerve cell bodies and processes in the canine small intestine show many similarities with other mammals, for example, in the distributions of VIP, substance P, neuropeptide Y, and somatostatin nerves. There are some major differences, such as the presence in dogs of numerous submucosal nerve cell bodies with enkephalinlike immunoreactivity and of GRP-like immunoreactivity in submucous nerve cell bodies and mucosal fibers.
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PMID:Peptide neurons in the canine small intestine. 241 66

The potency of several peptides and drugs as histamine liberators was assessed using the rat isolated hind limb preparation. Neurotensin (NT) and compound 48/80 (C48/80) were effective in concentrations as low as 10(-9) M and 10(-8) M, respectively. Threshold concentrations of vasoactive intestinal peptide (VIP) and substance P (SP) varied between 5 X 10(-7) to 5 X 10(-6) M while somatostatin (SS) was barely active at 6 X 10(-6) M. No histamine release could be detected following the use of high concentrations of thyrotropin releasing hormone (TRH) (6 X 10(-6) M), dynorphin (DYN) (6 X 10(-6) M) bradykinin (BK), des-Arg9-BK or bombesin (BB) (at 10(-5) M). Poly-L-Lysine and the calcium ionophore A23187 were about 100 times less active than NT. Concanavalin A (Con A) was inactive at 10(-6) M. These results indicate that NT is more potent (on a molar basis) as histamine liberator in the rat hind limb preparation (which contains a large population of cutaneous and subcutaneous mast cells) than any of the other compounds tested. Histamine release by NT was inhibited by preexposure of the rat hind limb mast cells to a high concentration of SP (1.5 X 10(-6) M). This result adds further support to the hypothesis suggesting that NT and SP might share a common mechanism of action and/or act through common receptors at least in rat mast cells.
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PMID:Potency of various peptides as histamine liberators in the rat hind limb. 241 86

The distribution of mucosal nerve fibres containing vasoactive intestinal peptide (VIP), substance P, somatostatin, neuropeptide Y (NPY), and enkephalinlike immunoreactivity was mapped by conventional immunohistochemical techniques throughout the mucosa of the esophagus, stomach, small and large intestines, and gall bladder. In addition, the distributions of endocrine cells immunoreactive for three peptides localized by these antisera (namely somatostatin, pancreatic polypeptide, and substance P) were recorded. Tissues from guinea pigs, rats, dogs, marmosets, and humans were studied. It was hoped that this information would enable possible target tissues and functional roles for the peptides to be identified. In the mucosa, peptide nerve fibres were found throughout the lamina propria, including some which were close to the epithelium and others associated with small blood vessels. Although there was a general similarity of peptide nerve distribution between regions and species, many small variations were observed. VIP and substance P fibres were the most prevalent nerve type; NPY fibres were also usually quite common. The distribution of somatostatin fibres was extremely variable between regions and species, and enkephalin fibres were usually rare. Endocrine cells of open (flask- or pyramid-shaped) and closed (rounded) types were seen; basal cytoplasmic processes (of variable length) were seen on many cells immunoreactive for somatostatin or pancreatic polypeptide. Epithelial cells immunoreactive for substance P were seen in the dog, marmoset, and human. The distributions and shapes of endocrine cells varied widely between areas and species. These studies provide a basis for the correlation of nerve distribution with pharmacological and physiological studies.
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PMID:Distribution of certain peptide-containing nerve fibres and endocrine cells in the gastrointestinal mucosa in five mammalian species. 241 38

Powerful regulatory peptides have been found in nerves and cells of peripheral tissues. The lung has been found to contain almost all the active peptides previously described. The respiratory tracts of three mammalian species--rat, guinea pig, and cat--were examined, and significant quantities of vasoactive intestinal peptide (VIP), bombesin, substance P, somatostatin, and cholecystokinin were found. The VIP nerves were most numerous in the upper respiratory tract, particularly the nasal mucosa. A close association with seromucous glands, blood vessels, and bronchial smooth muscle was particularly noteworthy and paralleled the pharmacologic actions of VIP, i.e., secretomotor, vasodilatory, and smooth muscle relaxation. In contrast, bombesin was localized to epithelial amine precursor uptake and decarboxylation (APUD) cells, which were particularly numerous in the fetus, suggesting a possible role in control of pulmonary growth. Substance P, a peptide thought to subserve a sensory role, was localized to fine nerve fibers, with a particularly close association with bronchial epithelium. The quantities of somatostatin and cholecystokinin were very low and, therefore, difficult to localize. In the human, bombesin and VIP were present in considerable quantities. Bombesin cells were again most numerous in the fetal and neonatal small bronchi and bronchiolar epithelium. During development VIP-ergic nerves showed little change in number but demonstrated a gradient of distribution, with the largest quantities located in the extrapulmonary airways. In preliminary investigations of pulmonary disease, bombesin levels were found to be very greatly reduced with acute hyaline membrane disease in the newborn.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Regulatory peptides and the lung. 241 6

The effects of six gastrointestinal regulatory peptides (beta-endorphin, substance P, metenkephalin, vasoactive intestinal peptide, bombesin, and somatostatin) on mouse lymphocytes stimulated with concanavalin A, lipopolysaccharide, phytohemagglutinin, or alloantigens were evaluated. Lymphocytes were stimulated in vitro and the influences of exogenously adding varying concentrations of neuropeptides (10(-6)-10(-11) M) on the incorporation of [methyl-3H-]thymidine were determined. The roles of cell density and antigen concentration on neuropeptide induced immunomodulation were also assessed. We observed that vasoactive intestinal peptide (VIP) would significantly inhibit the response of B10 lymphocytes to concanavalin A (54%) and phytohemagglutinin (56%) but not to lipopolysaccharide (16%). The VIP-induced inhibition was progressively diminished as the neuropeptide concentration was reduced to 10(-11) M. By 24 hr after stimulation the lymph node cells were refractory to the inhibitory effects of VIP. In addition, VIP would not inhibit B10 lymph node cells from responding to B10. K spleen cells in mixed, one-way lymphocyte cultures. The other five peptides did not influence the in vitro responses. The potential role of neuropeptides in the pathophysiology of immunologic-based disorders is discussed.
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PMID:Gastrointestinal regulatory peptides modulate in vitro immune reactions of mouse lymphoid cells. 242 53


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