Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P61278 (
somatostatin
)
22,083
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Neuropeptide FF
(
NPFF
) is a family member of RF-amide peptides, which are suggested to be involved in the control of vertebrate feeding behavior. However, little is known about the effect of the
NPFF
peptide on feeding-related processes in basal vertebrates. In this study, four full-length cDNAs,
npff
,
npffr1, npffr2-1
, and
npffr2-2
, were cloned from spotted sea bass and characterized. The conserved
NPFF
peptide is biologically active because it functionally interacts with different receptors expressed in cultured eukaryotic cells to enhance CRE promoter activity. Tissue distribution analysis showed that the highest
npff
mRNA expression occurred in the telencephalon, hypothalamus, medulla, gonad and muscle, but the
npffr
s mRNAs were mainly distributed within the central nervous system (CNS).
In situ
hybridization (ISH) detected
npff
-expressing cells in several specific regions ranging across the telencephalon and midbrain to the hypothalamus. Incubation of the spotted sea bass conserved
NPFF
peptide significantly increased the expression of
orexin
(
orx
) and
neuropeptide Y
(
npy
) mRNA and decreased the expression of
leptin
(
lep
),
somatostatin
(
ss
), and
cholecystokinin
(
cck
) mRNA in brain cells. Similarly, the conserved
NPFF
peptide also heightened the expression of
gastrin
(
gas
),
ghrelin
(
ghrl
), and
motilin
(
mtl
) mRNA and significantly reduced the expression of
cck
mRNA in the intestine and stomach. Taken together, these data suggest that the
NPFF
peptide may play a stimulating role in regulating feeding-related processes in spotted sea bass.
...
PMID:Evidence for the Direct Effect of the NPFF Peptide on the Expression of Feeding-Related Factors in Spotted Sea Bass (
Lateolabrax maculatus
). 3144 87
