Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P61278 (somatostatin)
 22,083 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The histochemical binding sites of peanut agglutinin (PNA) to normal foetal and neonatal pancreas and to pancreatic tissue from two cases with persistent neonatal hyperinsulinaemic hypoglycaemia were studied. For the demonstration of masked PNA binding sites, neuraminidase digestion was utilized. In addition, pancreatic endocrine cells were identified by immunocytochemistry for insulin, glucagon and somatostatin. Likewise, double immunohistochemical staining for simultaneous visualization of PNA binding sites and insulin reactive cells was employed. In pancreatic exocrine tissue, PNA binding occurred in all cases without neuraminidase treatment. Insular endocrine cells expressed PNA binding both in normal neonatal cases and in those with hyperinsulinaemic hypoglycaemia, but only after neuraminidase treatment. In foetal pancreas, no PNA binding was found on endocrine cells even after neuraminidase treatment, and it was also absent from B-cells in areas of the normal neonatal pancreas that showed the characteristic picture of nesidioblastosis. The possible mechanisms involved in determining the different pattern of PNA reactivity in foetal and neonatal endocrine pancreas are considered.
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PMID:Peanut lectin binding sites in human foetal and neonatal pancreas. 751 May 39

The goal of the study was to establish the age-related responses of cultured porcine pituitary cells to growth hormone-releasing factor (GRF) and(or) somatostatin (SRIF). A culture system for dispersed porcine pituitary cells was validated. Pituitaries from female pigs of various ages (90 or 110 d of gestation, newborn, 3, 6, or 24 mo old) were enzymatically dispersed with collagenase and neuraminidase, plated (200,000 cells/well), and cultured for 3 d. Plated cells were then subjected to a 4-h challenge with increasing concentrations of GRF (10(-11) to 10(-8) M), SRIF (10(-9) to 10(-6) M), or 10(-8) M of each peptide with increasing concentrations of the other. Culture media were collected and assayed for growth hormone (GH). Pituitaries were pooled so that there were four replicates per age, and treatments were assigned to quadruplicate wells. Concentrations of GH in control wells (basal GH) were maximal at 110 d of gestation and decreased thereafter (P < .01) with increasing age of swine. All peptide combinations affected the GH response (P < .05) at all ages studied, yet GRF was more potent than SRIF in eliciting a response. Age had an effect (P < .05) on the GH response to any of the treatments; younger pigs (90, 110 d of gestation and newborns) had a greater response (P < .05) than older pigs (3, 6, and 24 mo), whereas 6- and 24-mo-old pigs responded similarly in all cases (P > .1).(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Validation of a culture system for porcine pituitary cells: effects of growth hormone-releasing factor and(or) somatostatin on growth hormone secretion. 809 10

This study investigated whether neuraminidase (Neu) affects LFA-1 mRNA expression in spleen cells and whether somatostatin (SOM) and substance P (SP) treatment induce changes in the Neu mRNA expression level in spleen cells. Neu treatments down-regulated the LFA-1 mRNA levels after culturing for 2 h. SOM increased the Neu mRNA level slightly after 24-h culture and strongly after 48-h culture. These results suggest that prolonged exposure to SOM may regulate the Neu activation pathway, which in turn impairs the regulation of LFA-1 expression.
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PMID:Somatostatin controls LFA-1 gene expression by altering neuraminidase expression in spleen cells. 1533 Jan 80