Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P61278 (somatostatin)
 22,083 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The role of cytoskeletal microtubules and microfilaments in modulating cAMP generation in S49 lymphoma cells was investigated using the agents colchicine and cytochalasin B, respectively, which are known to disrupt these structures. A 1-hr pretreatment of S49 cells with 10 microM colchicine typically enhanced maximal isoproterenol-(beta-adrenergic receptor) stimulated cAMP accumulation by 100%, whereas cytochalasin B increased isoproterenol-stimulated cAMP by 30%. The combination of colchicine and cytochalasin B synergistically enhanced agonist-stimulated cAMP to 225% over control values. A synergistic increase in cAMP accumulation was also observed in cells treated with the agonist prostaglandin E1 or cholera toxin (which activates the stimulatory guanine nucleotide regulatory (Gs) protein). Colchicine and cytochalasin B did not ablate the inhibitory effects of somatostatin or the stimulatory effect of pertussis toxin treatment on beta-receptor-stimulated cAMP accumulation, indicating that these cytoskeletal disrupting agents do not enhance responsiveness in S49 cells via alterations in the inhibitory guanine nucleotide regulatory protein pathway. Moreover, colchicine, but not cytochalasin B treatment, enhances expression of isoproterenol-promoted 3H-forskolin binding in intact cells (a measure of Gs/adenylyl cyclase coupling). Thus, colchicine and cytochalasin B appear to enhance signaling in the Gs/adenylyl cyclase pathway by alterations of components distal to hormone receptors, most likely at the Gs protein and/or via cAMP generation. These results imply that microtubules and microfilaments can interact in the regulation of this pathway and that increases in cellular cAMP may contribute to the action of drugs that alter function of these cytoskeletal elements.
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PMID:Colchicine and cytochalasin B enhance cyclic AMP accumulation via postreceptor actions. 763 57

The distribution of calcitonin gene-related peptide (CGRP)-, vasoactive intestinal polypeptide (VIP)-, and somatostatin (SOM)-like immunoreactivities (-LI) in neurons of the spinal cord of developing chickens was characterized by use of the indirect immunofluorescence technique, and the findings related to a possible role for these peptides in the development of muscles and motor endplates. CGRP-LI in presumptive motoneurons of the ventral horn was first observed at embryonic day 6. During the following days the number of CGRP-immunoreactive (IR) cells increased reaching high numbers between days 12 and 18 of incubation, and thereafter decreasing in numbers until hatching. SOM-LI was first observed on embryonic day 7, while VIP-LI appeared on days 12 - 13. The number of SOM- and VIP-IR cells was considerably lower than that observed for CGRP-LI, but they also exhibited an initial increase followed by a decrease towards hatching. Intrathecal administration of colchicine increased the number of CGRP-IR motoneurons at days 7 and 30 after hatching and of VIP-IR ones at day 7, while at day 30 no expression of VIP-LI was found. Colchicine treatment did not cause any significant change in the number of SOM-IR motoneurons after hatching. The effect of VIP, SOM, and CGRP on cAMP accumulation in primary cultures of chick muscle cells was determined after labelling of the cells by (2-3H) adenine and by radioimmunoassay. All three peptides stimulated the accumulation of cAMP. However, the development of the pharmacological response of each of the peptides followed a different time course during in vitro differentiation of the primary cultures. The response of CGRP was the latest to develop and did not significantly decrease after the maximal response had been reached around day 3. The data are discussed in terms of 'trophic' effects of these neuropeptides upon muscle and spinal cord differentiation and synaptogenesis.
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PMID:Immunoreactive Calcitonin Gene-Related Peptide, Vasoactive Intestinal Polypeptide, and Somatostatin in Developing Chicken Spinal Cord Motoneurons. 1210 58


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