Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P61278 (somatostatin)
 22,083 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Dissolving basic peptides such as insulin, secretin, and somatostatin in albumin-containing solutions increases the biologic potency. As CCK-33 also constitutes a basic peptide, it should have similar qualities. In four conscious dogs with chronic gastric and pancreatic Thomas fistulas, we studied the effect of CCK-33 solutions with and without addition of albumin on pancreatic secretin, plasma PP concentration, CCK-like immunoreactivity in plasma and infusion lines. The response of pancreatic protein output (mg/15 min) to increasing doses of 99% pure CCK-33 (0.5; 1; 2; 4 IDU/kg/h) was significantly higher (p less than 0.05) when CCK was dissolved in 0.154 M NaCl with albumin than in NaCl alone. These results were confirmed by measuring plasma CCK-immunoreactivity by CCK-RIA and CCK-immunoreactivity in samples from tips of infusion lines by gastrin-RIA. CCK evoked PP release at increasing doses of CCK-33, which was significantly (p less than 0.05) higher when CCK was dissolved in an albumin-containing solution. There was a significant (p less than 0.02) correlation between plasma PP concentration and pancreatic protein output. Our study shows addition of albumin to solutions of CCK-33 to cause an increase of CCK-33 activity, probably by preventing adsorption of the peptide to glass and plastic surfaces. This is valid for its effect on pancreatic protein secretion as well as PP release.
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PMID:[Addition of albumin to solutions of cholecystokinin (CCK)--effect on pancreatic secretion, plasma CCK level and release of pancreatic polypeptide (PP)]. 686 20