Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P61278 (somatostatin)
 22,083 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We describe the characterization of somatostatin-like immunoreactivity (SRIF-LI) found by radioimmunoassay (RIA) to be present in normal human serum. Degradation by serum of 125I-Tyr1 SRIF in the assay, as assessed by chromatoelectrophoresis and immunoprecipitation, was overcome by using EDTA in the assay buffer and Trasylol in the blood samples. Serum samples thus obtained from 48 normal subjects revealed a bimodal distribution of SRIF-LI; 92 per cent (group 1) had a mean level of 0.274 +/- 0.009 ng. per milliliter. What was measured in these sera showed identity to synthetic SRIF on serial dilutions, Sephadex G-25 chromatography, and thin-layer chromatography, and it was shown to be immunoreactive by an antibody-Sepharose affinity system. Higher levels (1.0 +/- 0.041 ng. per milliliter) were found in 8 per cent of the sera; 50 per cent of this material behaved identically as serum SRIF-LI from group 1. The remainder proved to be heterogeneous, consisting of two peaks of large molecular weight, both of which shared immunologic identity with synthetic SRIF as shown by binding to the antibody-Sepharose affinity system. Their further nature is unknown.
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PMID:The characterization of somatostatin-like immunoreactivity in human serum. 30 57

Somatostatin is an ubiqutary neuropeptide and hormone which has been reported to exert hemodynamic effects and to bind to receptors on the red cell membrane. We investigated its effects on red cell deformability by three filtration methods: (a) filtration of red cells resuspended at hematocrit 8% in Tris-Albumin-Glucose buffer under atmospheric pressure; (b) filtration of red cells resuspended at hematocrit on native plasma at 8% hematocrit under a negative pressure of 5 cm of water; (c) filtrability of whole blood under a negative pressure of 20 cm of water. Aprotinin (Antagosan*) was added to the different suspensions in order to avoid rapid destruction of somatostatin. Increased quantities of somatostatin (from 1 pg/ml to 1 microgram/ml) were obtained by adding natural somatostatin (Modustatin*) to the media, before they were incubated at 37 C for 30 minutes. In 11 samples from healthy subjects, somatostatin was shown to increase red cell flow rate in technique (c) (+ 118%, p less than 0.05) and to reduce red cell rigidity index in technique (b) (- 71%, p less than 0.025) whereas a nonsignificant similar tendency (- 56%) was observed with technique (a). Similar results (p less than 0.05) are observed when adding somatostatin to blood of diabetics. These in vitro data suggest that somatostatin, like other previously studied hormones, may modify red cell deformability.
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PMID:In vitro effects of somatostatin on red cell filterability measured by three methods. 167 5

Aprotinin was found to inhibit the amplitude of electrically stimulated contractions of the isolated guinea pig ileum with an ID50 of 6.75 X 10(-6) M. Aprotinin inhibited pentagastrin-stimulated gastric acid secretion in the rat, cat, and pig after oral or intraventricular administration. No inhibition of gastric acid secretion was seen after intravenous infusion of aprotinin. When segments of antrum, duodenum, and ileum were incubated in vitro with increasing concentrations of aprotinin, increasing amounts of somatostatin were released from the organs. It is suggested that aprotinin's effects, which are similar to those of somatostatin, may be due to a local release of somatostatin from the D-cells in the gastrointestinal tract.
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PMID:Inhibitory effect of aprotinin on gastric acid secretion. 608 20